Myricetin Isolated From Turbinaria Ornata Ameliorates Rotenone Induced Parkinsonism in Drosophila Melanogaster

Abstract

Objective: Parkinson's disease (PD) is a neurodegenerative disorder which affects the elderly population. Free radicals overproduction, oxidative stress, apoptosis, inflammation and abnormalities in mitochondria are critical mediators of the neuronal degeneration. In the present study neuroprotective activity of myricetin, a flavonoid isolated from brown seaweed Turbinaria ornata have been investigated in rotenone induced experimental PD models of Drosophila melanogaster. Methods:Male fruit flies (Drosophila melanogaster) were fed with an effective dose of 0.1% myricetin three hours before to the treatment with 500 µM of Rotenone (LD 50) for seven days and on 8 thResults: Myricetin maintains the positive behavioral patterns against motor impairments due to the rotenone toxicity, it creates a balance in oxidant and antioxidant status, reduces the oxidative stress and inhibits apoptosis to retard neurodegeneration and maintains the dopamine level with a significant (p<0.05) difference compared to the rotenone treated group. day through behavioral analysis the neuroprotective effect of myricetin was investigated for motor coordination in fruit flies. Lipid peroxidation was analyzed by estimating the levels of TBARS. Oxidative stress was determined by estimating the activities of enzymatic antioxidants superoxide dismutase, catalase, and glutathione peroxidase along with the level of reduced glutathione. Dopamine level was estimated in HPLC column detected at 280 nm with UV detectors and degree of apoptosis was studied apoptotic marker Bcl-2, Bax, caspases-3 and 9, cytochrome c and β-actin expressions in the whole body homogenate of fruit flies of experimental groups homogenized in 500μL of 0.1 M phosphate buffers (ice cold, pH, 7.4) containing 1 mmol EDTA. Conclusion:The flavonoid myricetin by reducing the oxidative stress, maintaining the enzymatic antioxidants status and by inhibiting apoptosis prevents the degeneration of dopaminergic neurons. The dopaminergic neurons prevention reduces the depletion of dopamine and thereby promotes the muscular coordination and psychological well being of fruit flies of experimental group. Further in depth molecular level studies are in need to explore the preventive mechanisms of myricetin in Parkinson's disease.

Chronic Unpredictable Stress-Induced Behavioral and Electrophysiological Alterations and Its Ameliorative Effect by Myricetin Microemulsion

“…Further, it is confirmed that MYR has capable of inhibiting the glutamate release in the cortical nerve terminal by suppressing of presynaptic Ca 2+ influx [35]. Recent findings reported that MYR has capable of ameliorates rotenone-induced parkinsonism in drosophila [36]. Malathi et al also reported that animals which were exposed to chronic restraint stress showed impaired learning and memory which was restored by Michelia champaca extract [37].…”

Pharmacological Activities of Natural Products from Marine Seaweed Turbinaria ornata: A Review

“…A flavonoid isolated from T. ornata in rotenone stimulated Parkinson's disease models of Drosophila melanogaster has been checked for the neuroprotective activity of myricetin. Myricetin produces a balance of oxidants and antioxidants, decreases oxidative stress, and inhibits apoptosis to delay neurodegeneration and sustain the dopamine level [66]. More molecular studies will be needed in the future to study myricetin defence processes in Parkinson's disease.…”

“…The several types of phenolic compounds present in the extract act as a dietary supplement and were found to relieve joint-related pain [65]. The methanolic extract was also found to have a protective action on the red blood cells [66]. Another study found that the ethanolic extracts exhibited excellent vascular inhibition in duck CAM assay and serve as potential cytotoxic agents [68].…”

In Vitro Antioxidant and Cytotoxic Properties of Fucoidan From Three Indian Brown Seaweeds

“…Although the ethanolic extract of P. gymnospora has been reported to contain a number of bioactives compared to many other algae, its sulfate content and antioxidant activity were found lower comparatively [45]. The antioxidant and FRSA of the methanolic extract of T. ornata are also already known [46]. The demand for seaweeds has enormously increased recently as it is a source of numerous bioactive compounds that are targeted for biomedical applications as well as the food industry [47].…”