Myotube formation on gelatin nanofibers – Multi-walled carbon nanotubes hybrid scaffolds

Ostrovidov, Serge; Shi, Xuetao; Zhang, Ling; Liang, Xiaobin; Kim, Sang Bok; Fujie, Toshinori; Ramalingam, Murugan; Chen, Luyang; Nakajima, Ken; Al-Hazmi, Faten; Bae, Hojae; Memić, Adnan; Khademhosseini, Ali

doi:10.1016/j.biomaterials.2014.04.021

Cited by 109 publications

(77 citation statements)

References 64 publications

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“…To generate micro- and nanoscale electrospun fibers, a stream of a prepolymer solution is subjected to a high electrical field, and the fibers are then gathered on a collector, yielding fibrous constructs [26]. In addition to the choice of polymer, experimental electrospinning conditions, including the applied electrical field, solution flow rate and viscosity, can affect the physical properties (e.g., mechanical properties and microarchitecture) of the generated nanofibrous mats [27,28,29]. Flexible electrospun mats have previously been used for various applications, including tissue-engineered constructs and drug delivery platforms for a range of drugs.…”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Silver-Coated Polymeric Scaffolds with Tunable Electrical Properties

et al. 2017

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Electrospun micro- and nanofibrous poly(glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) substrates have been extensively used as scaffolds for engineered tissues due to their desirable mechanical properties and their tunable degradability. In this study, we fabricated micro/nanofibrous scaffolds from a PGS-PCL composite using a standard electrospinning approach and then coated them with silver (Ag) using a custom radio frequency (RF) sputtering method. The Ag coating formed an electrically conductive layer around the fibers and decreased the pore size. The thickness of the Ag coating could be controlled, thereby tailoring the conductivity of the substrate. The flexible, stretchable patches formed excellent conformal contact with surrounding tissues and possessed excellent pattern-substrate fidelity. In vitro studies confirmed the platform’s biocompatibility and biodegradability. Finally, the potential controlled release of the Ag coating from the composite fibrous scaffolds could be beneficial for many clinical applications.

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Section: Introductionmentioning

confidence: 99%

Nanofibrous Silver-Coated Polymeric Scaffolds with Tunable Electrical Properties

et al. 2017

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“…In addition, and similarly to bone regeneration, collagen-supporting calcium phosphate nanoparticles have been shown to be efficient agents in treat wound healing thanks to their controlled release of ions [189]. [202] Cartilage is considered a non-vascular tissue, as the blood sup-ply is limited, and nerve and lymphatic vessels are scarce;…”

Section: Soft Tissuesmentioning

confidence: 99%

“…Muscle tissue engineering needs the fabrication of packed, dense, aligned and mature myotubes [202]. Multi-wall nanotube (MWNT)-gelatin hybrid fibers were produced by electrospinning, and allowed the efficient alignment and differentiation of myoblasts to offer functional myotubes.…”

Section: Soft Tissuesmentioning

confidence: 99%

Hybrid Organic-Inorganic Scaffolding Biomaterials for Regenerative Therapies

Sachot¹,

Engel²,

Castaño

2014

COC

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Abstract:The introduction of hybrid materials in regenerative medicine has solved some problems related to the mechanical and bioactive properties of biomaterials. Calcium phosphates and their derivatives have provided the basis for inorganic components, thanks to their good bioactivity, especially in bone regeneration. When mixed with biodegradable polymers, the result is a synergic association that mimics the composition of many tissues of the human body and, additionally, exhibits suitable mechanical properties. Together with the development of nanotechnology and new synthesis methods, hybrids offer a promising option for the development of a third or fourth generation of smart biomaterials and scaffolds to guide the regeneration of natural tissues, with an optimum efficiency/cost ratio. Their potential bioactivity, as well as other valuable features of hybrids, open promising new pathways for their usein bone regeneration and other tissue repair therapies. This review provides a comprehensive overview of the different hybrid organic-inorganic scaffolding bio-materials developed so far for regenerative therapies, especially in bone. It also looks at the potential for research into hybrid materials for other, softer tissues, which is still at an initial stage, but with very promising results.

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“…The electrical stimulation is suitable especially for cells of excitable tissues, such as neural tissue [80] (for a review, see [81]), myocardium [82], skeletal muscle [38], and vascular smooth muscle [83]. The addition of carbon nanoparticles also improved the mechanical properties of the nanofibrous scaffolds for engineering of muscular tissues, which are exposed to a relatively high mechanical loading in the organism.…”

Section: Introductionmentioning

confidence: 99%

“…However, pure polymeric nanofibers are suitable mainly for soft tissue engineering, such as reconstruction and regeneration of blood vessels [13,22,23,31,32], myocardium [33,34], heart valves [35,36], skeletal muscle [37,38], skin [15, [39][40][41], tendon and ligament [42,43], intestine [44,45], tissues of the respiratory system, such as trachea and bronchi [46,47], components of urinary tract, such as bladder [48] and urethra [49], visceral organs, such as liver [50,51] or pancreas (pancreatic islets [52,53]), central nervous system, such as brain [6,54,55], spinal cord [56,57], optic system, such as optical nerve [58] and retina [59], and peripheral nervous system [17,60]. Nanofibrous scaffolds can be associated with another advanced technique in recent tissue engineering-controlled delivery of various types of stem cells, such as bone marrow mesenchymal stem cells [51,[61][62][63], adipose tissue-derived stem cells [64,65], neural tissue-derived stem cells [57], and induced pluripotent stem cells …”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

Bačáková¹,

Bačáková²,

Pajorová³

et al. 2016

Nanofiber Research - Reaching New Heights

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Nanofibers are promising cell carriers for tissue engineering of a variety of tissues and organs in the human organism. They have been experimentally used for reconstruction of tissues of cardiovascular, respiratory, digestive, urinary, nervous and musculoskeletal systems. Nanofibers are also promising for drug and gene delivery, construction of biosensors and biostimulators, and wound dressings. Nanofibers can be created from a wide range of natural polymers or synthetic biostable and biodegradable polymers. For hard tissue engineering, polymeric nanofibers can be reinforced with various ceramic, metal-based or carbon-based nanoparticles, or created directly from hard materials. The nanofibrous scaffolds can be loaded with various bioactive molecules, such as growth, differentiation and angiogenic factors, or funcionalized with ligands for the cell adhesion receptors. This review also includes our experience in skin tissue engineering using nanofibers fabricated from polycaprolactone and its copolymer with polylactide, cellulose acetate, and particularly from polylactide nanofibers modified by plasma activation and fibrin coating. In addition, we studied the interaction of human bone-derived cells with nanofibrous scaffolds loaded with hydroxyapatite or diamond nanoparticles. We also created novel nanofibers based on diamond deposition on a SiO 2 template, and tested their effects on the adhesion, viability and growth of human vascular endothelial cells.

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Myotube formation on gelatin nanofibers – Multi-walled carbon nanotubes hybrid scaffolds

Cited by 109 publications

References 64 publications

Nanofibrous Silver-Coated Polymeric Scaffolds with Tunable Electrical Properties

Nanofibrous Silver-Coated Polymeric Scaffolds with Tunable Electrical Properties

Hybrid Organic-Inorganic Scaffolding Biomaterials for Regenerative Therapies

Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

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