Myelopeptide-5 is a novel endogenous immunoregulator recovering the functional activity of T-lymphocytes

Belevskaya, R. G.; Kalyuzhnaya, M. V.; Fonina, L. A.; Mikhailova, A. V.; Rv, Petrov

doi:10.1134/s0012496607050249

Cited by 3 publications

(8 citation statements)

References 6 publications

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“…This model is based on the evaluation of proliferation of T-lymphocytes of human peripheral blood cultured at suboptimal concentration of the mitogen. This property of the T-lymphocytes is characteristic of their functional activity [8].…”

Section: Resultsmentioning

confidence: 99%

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Synthesis and Properties of the retro-Analogue of Myelopeptide MP-2

et al. 2005

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The bone marrow myelopeptide MP-2 (Leu-Val-Val-Tyr-Pro-Trp), exhibiting antitumor activity, and its retro-analogue (Trp-Pro-Tyr-Val-Val-Leu) were synthesized, and their properties were studied. The in vitro and in vivo activities of retro-MP-2 were comparable with those of MP-2. Both peptides equally restored the functional activity of T-lymphocytes inhibited by toxins released by HL-60 cells and inhibited by 70-82% the growth of various types of transplantable solid tumors: Ca-755 adenocarcinoma of the mammary gland, Lewis adenocarcinoma of the lung, and S180 sarcoma. The positions and intensities of the Cotton effects in CD spectra of the MP-2 peptide and its retro-analogue in various solvents are almost indistinguishable. The positions of extrema and integral intensities of the amide I and amide A bands in IR spectra of both peptides were practically identical. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2005, vol. 31, no. 3; see also http://www.maik.ru.

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Section: Resultsmentioning

confidence: 99%

“…CM from the leukemia cells of the HL-60 line was used for the inhibition of the T-lymphocyte functional activity. The proliferative response of T-lymphocytes to the PHA introduction was evaluated according to the incorporation of [ 3 H]thymidine into DNA as described in [8]. Results of the experiments are presented in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Properties of the retro-Analogue of Myelopeptide MP-2

et al. 2005

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“…Previous in vitro experiments showed that MP-5 restored functional activity of T cells, suppressed by measles virus by stimulating the reduced production of endogenous IL-2 and the expression of IL-2 receptor [1]. Now we studied the antisuppressive effect of MP-5 in vivo.…”

Section: Anti-influenza Vaccine (Liv) Contains Influenza Amentioning

confidence: 95%

“…In previous studies, binding of human peripheral blood T cell CD4 + subpopulation to MP-5-FITC was evaluated and the dissociation constant was calculated (Kd=2.28×10 -7 M) [1]. In contrast to the fluorescent label, which can modulate spatial structure of the peptide molecule, radioactive label has no effect of this kind, and [ 3 H]MP-5 is in fact quite identical to MP-5.…”

Section: Anti-influenza Vaccine (Liv) Contains Influenza Amentioning

confidence: 99%

“…They maintain the stability of the immune status due to immunocorrective activity towards certain target cells, binding to them through ligand-receptor interactions [4]. It was shown that MP-5 (synthetic analog of endogenous low-molecular-weight Val-Val-Tyr-Pro-Asp peptide) restored functional activity of T cells, suppressed by tumor cell metabolites or measles virus by stimulating the production of endogenous IL-2 and the expression of IL-2 receptors suppressed under the effects of these factors [1].…”

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confidence: 98%

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Myelopeptide-5 Abolishes Virus-Induced Immunosuppression by Restoring T Cell Functions

et al. 2009

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Myelopeptide-5 (MP-5; synthetic analog of endogenous low-molecular-weight peptide Val-Val-Tyr-Pro-Asp) neutralized the immunosuppressive effects of antiviral vaccines (influenza, measles, and measles/parotitis) and stimulated their immunogenicity by restoring functional activity of T cells, suppressed by the viruses. Specific binding of MP-5 to CD4(+) lymphocyte (its target cell) was studied using [(3)H]MP-5 (dissociation constant 2.03 x 10(-7) M).

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The efficiency of solvent‐free catalyst systems in the synthesis of tritium‐labelled biologically active compounds

Shevchenko

Nagaev

Myasoedov

2010

Labelled Comp Radiopharmac

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Modern theories about the processes that take place inside solvent‐free catalyst systems are considered and the various factors that have an effect on the successful introduction of tritium into organic compounds are summarized. Obtaining labelled compounds using solvent‐free catalyst systems, liquid‐phase methods, and isotopic exchange with tritiated water are all regarded as various manifestations of the same set of occurrences in the presence of tritium, a substrate and a catalyst. The use of various isotopic exchange methods has made possible the introduction of tritium into practically any type of biologically active compound. This will enable scientists to carry out more detailed studies of the processes inside living organisms. Copyright © 2010 John Wiley & Sons, Ltd.

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Myelopeptide-5 is a novel endogenous immunoregulator recovering the functional activity of T-lymphocytes

Cited by 3 publications

References 6 publications

Synthesis and Properties of the retro-Analogue of Myelopeptide MP-2

Synthesis and Properties of the retro-Analogue of Myelopeptide MP-2

Myelopeptide-5 Abolishes Virus-Induced Immunosuppression by Restoring T Cell Functions

The efficiency of solvent‐free catalyst systems in the synthesis of tritium‐labelled biologically active compounds

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