Mycoplasma ovipneumoniae infection in Zimbabwean goats and sheep

Mohan, Krishna; Obwolo, M. J.; Hill, F. W.

doi:10.1016/0021-9975(92)90097-e

Cited by 19 publications

(11 citation statements)

References 8 publications

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“…The organism was a common inhabitant of the upper respiratory tract of the goats prior to infection and was also cultivated from the lung suspension used as part of the inoculum. The possible contribution to lung disease by this agent is difficult to ascertain, but caprine M. ovipneumoniae infection is one possible cause of cuffing pneumonia [21], which commonly was a part of the pulmonary histopathology observed in our goats.…”

Section: Discussionmentioning

confidence: 99%

Experimental Contagious Caprine Pleuropneumonia: A Long Term Study on the Course of Infection and Pathology in a Flock of Goats Infected with Mycoplasma capricolum subsp. capripneumoniae

et al. 2004

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: Experimental contagious caprine pleuropneumonia: A long term study on the course of infection and pathology in a flock of goats infected with Mycoplasma capricolum subsp. capripneumoniae. Acta vet. scand. 2004, 45, 167-179. -Contagious caprine pleuropneumonia (CCPP) is a major threat to goat farming in parts of Africa and Asia. It classically causes acute high morbidity and mortality early in infection, but little is known of its long term epizootiology and course. In this study, 10 goats were inoculated with Mycoplasma capricolum subsp. capripneumoniae (M. capripneumoniae) and then mixed with 15 goats for contact transmission. The disease course was monitored in each goat for 56-105 days, whereafter the goats were killed and necropsied. Varying features signifying infection occurred in altogether 17 goats (7 inoculated, 10 in-contact). Clinical signs were severe in 8 goats but no fatalities occurred. Only 6 goats had serum antibody titres against M. capripneumoniae in ELISA. Fourteen goats (5 inoculated, 9 in-contact) had chronic pleuropulmonary lesions compatible with CCPP at necropsy and 7 of those showed M. capripneumoniae antigen in the lung by immunohistochemistry. Neither cultivation nor PCR tests were positive for the agent in any goat. The results indicate that the clinical course of CCPP in a flock may be comparatively mild, M. capripneumoniaeassociated lung lesions may be present at a late stage of infection, and chronic infection may occur without a significant serological response. goat; Mycoplasma; contagious pleuropneumonia; ELISA; immunohistochemistry; serology; pathology.

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Section: Discussionmentioning

confidence: 99%

Experimental Contagious Caprine Pleuropneumonia: A Long Term Study on the Course of Infection and Pathology in a Flock of Goats Infected with Mycoplasma capricolum subsp. capripneumoniae

et al. 2004

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“…Mycoplasma ovipneumoniae is one of the major pathogens that cause mycoplasma pneumonia in sheep, goats, and wild ruminants [1][2][3][4][5]. M. ovipneumoniae-associated respiratory disease is characterized by cough, gasp, runny noses, progressive weight loss, pulmonary interstitial hyperplasia inflammation, and variable morbidity and mortality rates between flocks [6,7].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of the isothermal recombinase polymerase amplification assays for rapid detection of Mycoplasma ovipneumoniae in sheep

Wang¹,

Sun³

et al. 2020

BMC Vet Res

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Background: Mycoplasmal pneumonia is an important infectious disease that threatens sheep and goat production worldwide, and Mycoplasma ovipneumoniae is one of major etiological agent causing mycoplasmal pneumonia. Recombinase polymerase amplification (RPA) is an isothermal nucleic acid amplification technique, and RPA-based diagnostic assays have been described for the detection of different types of pathogens. Results: The RPA assays using real-time fluorescence detection (real-time RPA) and lateral flow strip detection (LFS RPA) were developed to detect M. ovipneumoniae targeting a conserved region of the 16S rRNA gene. Real-time RPA was performed in a portable florescence scanner at 39°C for 20 min. LFS RPA was performed in a portable metal bath incubator at 39°C for 15 min, and the amplicons were visualized with the naked eyes within 5 min on the lateral flow strip. Both assays were highly specific for M. ovipneumoniae, as there were no cross-reactions with other microorganisms tested, especially the pathogens involved in respiratory complex and other mycoplasmas frequently identified in ruminants. The limit of detection of LFS RPA assay was 1.0 × 10 1 copies per reaction using a recombinant plasmid containing target gene as template, which is 10 times lower than the limit of detection of the real-time RPA and real-time PCR assays. The RPA assays were further validated on 111 clinical sheep nasal swab and fresh lung samples, and M. ovipneumoniae DNA was detected in 29 samples in the real-time RPA, 31 samples in the LFS RPA and 32 samples in the real-time PCR assay. Compared to real-time PCR, the real-time RPA and LFS RPA showed diagnostic specificity of 100 and 98.73%, diagnostic sensitivity of 90.63 and 93.75%, and a kappa coefficient of 0.932 and 0.934, respectively. Conclusions: The developed real-time RPA and LFS RPA assays provide the attractive and promising tools for rapid, convenient and reliable detection of M. ovipneumoniae in sheep, especially in resource-limited settings. However, the effectiveness of the developed RPA assays in the detection of M. ovipneumoniae in goats needs to be further validated.

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“…Several members of the genera Mycoplasma and Ureaplasma have been proven to be pathogens of the respiratory tract of sheep and goats, but fulfillment of Koch's postulates has been difficult Cassell, 1982;Gourlay and Howard, 1982;Mohan et al, 1992). Many mycoplasmas have been isolated from the upper respiratory tract of healthy or pneumonic sheep and goats or from their lungs with or without pneumonia (Jones, 1983;.…”

Section: Mycoplasma Of Sheep and Goatsmentioning

confidence: 99%

“…Many mycoplasmas have been isolated from the upper respiratory tract of healthy or pneumonic sheep and goats or from their lungs with or without pneumonia (Jones, 1983;. Mycoplasma ovipneumoniae has also been recovered from lymph nodes and thymus of experimentally infected kids and lambs (Mohan et al, 1992). A list of mycoplasmas isolated from sheep and goats and their biochemical characteristics is provided in not only affect females but also males, and many other mycoplasmas can produce mastitis leading to agalactia.…”

Section: Mycoplasma Of Sheep and Goatsmentioning

confidence: 99%

Respiratory infection of lambs with Mycoplasma ovipneumoniae

Khan

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Mycoplasma ovipneumoniae infection in Zimbabwean goats and sheep

Cited by 19 publications

References 8 publications

Experimental Contagious Caprine Pleuropneumonia: A Long Term Study on the Course of Infection and Pathology in a Flock of Goats Infected with Mycoplasma capricolum subsp. capripneumoniae

Experimental Contagious Caprine Pleuropneumonia: A Long Term Study on the Course of Infection and Pathology in a Flock of Goats Infected with Mycoplasma capricolum subsp. capripneumoniae

Development and validation of the isothermal recombinase polymerase amplification assays for rapid detection of Mycoplasma ovipneumoniae in sheep

Respiratory infection of lambs with Mycoplasma ovipneumoniae

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