Respiratory infection of lambs with Mycoplasma ovipneumoniae

Abstract

Table 2. Mycoplasma Associated Diseases' Agents Host Disease manifestations M. mycoidea ssp mycoidesCLC) M. capricolum M. hyorhinia M. aynoviae M. bovia M. hyoaynoviae M. arthritidia M. galliaepticum M. diapar U. diveraum M. ovipneumoniae M. hyopneumoniae M. pulmonia M. mycoides ssp mycoidea (SC) M. sp F-38 M. mycoidea ssp capri

Field Isolates of Mycoplasma ovipneumoniae Exhibit Distinct Cytopathic Effects in Ovine Tracheal Organ Cultures

“…Positive staining was not observed in any other areas, suggesting that M. ovipneumoniue colonizes only the ciliated epithelium. This is in agreement with the observations made by KHAN (1993), but contrasts with those made by HAZIROGLU et al (1996) who demonstrated the presence of M. ovipneumoniae antigens within the epithelial cells with an immunoperoxidase technique in pneumonic ovine lungs. Further evidence corroborating our findings were observations by TEM which showed close association of mycoplasma organisms only with the cilia.…”

“…ouipneunioniae R94 (a high passage strain) was derived from the R19 isolate by 94 in vztm passages in modified Friis broth medium (MFBM). The mycoplasmas were grown in MFBM (KHAN, 1993). Cultures were harvested and washed once in phosphate buffered saline (PBS, pH 7.4) by centrifugation at 10000 x g for 30min at 4°C.…”

“…For light microscopy, rings were fixed in 10% buffered neutral formalin, processed by routine paraffin embedding, microsectioned and stained with hematoxylin and eosin. Formalin-fixed tissue sections were also examined for the presence of M. ovipneumoniae antigen using an indirect immunohistochemical staining procedure described elsewhere (KHAN, 1993). For electron microscopy, rings were fixed with or without ruthenium red (Sigma Chemical Corporation, St. Louis, MO) at 4 O C in 3% glutaraldehyde in 0.1 M sodium cacodylate buffer @H 7.2), processed as previously described (DEBEY and ROSS, 1994), and thin sections were examined by transmission electron microscopy (TEM) using a Hitachi H500 TEM at 75 kV.…”

Differential Serologic Response to Mycoplasma Ovipneumoniae and Mycoplasma Arginini in Lambs Affected with Chronic Respiratory Disease

“…MO and MA organisms were grown in modified Friis broth medium for 3 days at 37 C as previously described. 7 Cultures were harvested and washed 3 times in phosphate-buffered saline (PBS, pH 7.4) by centrifugation at 10,000 ϫ g at 4 C for 30 min. The protein concentration of washed mycoplasmas was determined with a commercial kit a following the instructions of the manufacturer, and aliquots were stored at Ϫ70 C.…”

“…There is some evidence that the extended persistence of the MO organism in the respiratory tract of these lambs and the chronic nature of the disease may be due to failure of the immune system to generate protective immunity. 10 Reasons for that failure are not known, but marked variation in the MO organisms isolated from sheep 7 and expression of a polysaccharide capsule by the organisms are recognized. 11 Because of its simplicity the enzyme-linked immunosorbent assay (ELISA) has been extensively used in detecting antibodies to Mycoplasma species.…”