Expression of functions by normal sheep alveolar macrophages and their alteration by interaction with Mycoplasma ovipneumoniae

Niang, Mamadou; Rosenbusch, Ricardo F.; Lopez-Virella, Jose; Kaeberle, Merlin L.

doi:10.1016/s0378-1135(97)00141-7

Cited by 22 publications

(11 citation statements)

References 21 publications

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“…They also participate in homeostasis in their local tissue microenvironment, a function of specialized tissue-resident macrophages in essentially every organ in the body ( Lavin et al, 2014 ). Macrophages can be hijacked by pathogens like Mycobacterium bovis , Ovine lentivirus, Coxiella burnetii , Mycoplasma ovipneumoniae , Brucella melitensis , and Salmonella enterica , that cause zoonotic and economically important diseases in sheep: tuberculosis, ovine progressive pneumonia, Q-fever, atypical pneumonia (part of respiratory disease complex), brucellosis, and salmonellosis, respectively ( Gendelman et al, 1986 ; Niang, 1992 ; Niang et al, 1997 ; Shannon and Heinzen, 2009 ; Blacklaws, 2012 ; Hall et al, 2020 ). Many of these infectious agents are intracellular organisms that can sequester within host macrophages from the full force of the immune system and manipulate antigen processing and presentation.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Histone Modifications and CTCF Enrichment Predict Gene Expression in Sheep Macrophages

et al. 2021

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Alveolar macrophages function in innate and adaptive immunity, wound healing, and homeostasis in the lungs dependent on tissue-specific gene expression under epigenetic regulation. The functional diversity of tissue resident macrophages, despite their common myeloid lineage, highlights the need to study tissue-specific regulatory elements that control gene expression. Increasing evidence supports the hypothesis that subtle genetic changes alter sheep macrophage response to important production pathogens and zoonoses, for example, viruses like small ruminant lentiviruses and bacteria like Coxiella burnetii. Annotation of transcriptional regulatory elements will aid researchers in identifying genetic mutations of immunological consequence. Here we report the first genome-wide survey of regulatory elements in any sheep immune cell, utilizing alveolar macrophages. We assayed histone modifications and CTCF enrichment by chromatin immunoprecipitation with deep sequencing (ChIP-seq) in two sheep to determine cis-regulatory DNA elements and chromatin domain boundaries that control immunity-related gene expression. Histone modifications included H3K4me3 (denoting active promoters), H3K27ac (active enhancers), H3K4me1 (primed and distal enhancers), and H3K27me3 (broad silencers). In total, we identified 248,674 reproducible regulatory elements, which allowed assignment of putative biological function in macrophages to 12% of the sheep genome. Data exceeded the FAANG and ENCODE standards of 20 million and 45 million useable fragments for narrow and broad marks, respectively. Active elements showed consensus with RNA-seq data and were predictive of gene expression in alveolar macrophages from the publicly available Sheep Gene Expression Atlas. Silencer elements were not enriched for expressed genes, but rather for repressed developmental genes. CTCF enrichment enabled identification of 11,000 chromatin domains with mean size of 258 kb. To our knowledge, this is the first report to use immunoprecipitated CTCF to determine putative topological domains in sheep immune cells. Furthermore, these data will empower phenotype-associated mutation discovery since most causal variants are within regulatory elements.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Histone Modifications and CTCF Enrichment Predict Gene Expression in Sheep Macrophages

et al. 2021

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“…Current control measures, which include the use of anti-mycoplasma antibiotics, impair the actual status of Movp infections (Gonçalves and others 2010). Characteristics of this mycoplasma species, such as extensive protein, antigenic and genetic heterogeneity (Thirkell and others 1990, Ionas and others 1991, Parham and others 2006, Harvey and others 2007), alterations in macrophage activity (Niang and others 1997), induction of the production of autoantibodies to the ciliary antigen (Niang and others 1998) and suppressive activity on lymphocytes (Shahzad and others 2010) underlie its capability to avoid the host immune response and increase difficulties in its diagnosis. In addition, Movp is frequently isolated from pneumonic and healthy sheep (DaMassa and others 1992).…”

Section: Introductionmentioning

confidence: 99%

Comparison of phenotypic and genotypic profiles among caprine and ovineMycoplasma ovipneumoniaestrains

Maksimović

Amores

et al. 2017

Veterinary Record

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() is considered to be one of the most important mycoplasmas causing respiratory disease in small ruminants. Most epidemiologic and characterisation studies have been conducted on strains collected from sheep. Information on the presence and characteristics of in healthy and pneumonic goats is limited. Phenotypic or genotypic differences between sheep and goat isolates have never been studied. The objective of our study was to characterise and compare the similarities and differences between caprine and ovine strains isolated from affected and asymptomatic animals in order to elucidate phenotypic and genotypic variability. Four different techniques were used on a set of 23 isolates. These included SDS-PAGE, Western blotting, random amplified polymorphic DNA and the heat shock protein 70 gene sequence-based method. A high degree of phenotypic and genotypic heterogeneity among strains was demonstrated in this study. Our results demonstrated differences between goat and sheep strains, revealing not only a link between strains and host ruminant species, but by geographical origin as well. However, the finding of immunodominant antigens of molecular masses 36, 38, 40 and 70 kDa (±3 kDa) in isolates from sheep and goats foretells their potential use in the development of serological diagnostic tests and vaccines.

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“…Mycoplasma ovipneumoniae ( M. ovipneumoniae ) is an etiological pathogen that colonizes respiratory epithelial cells of sheep exposed to contaminated air and is known to be a major contributing factor to the occurrence of primary atypical pneumonia [2, 3, 10–13]. Polysaccharides, polymers of repeated mono- or di-oligosaccharide residues joined together by glycosidic bonds, are main component of the outer capsule of M. ovipneumoniae which play significant roles in its growth and development [14–16].…”

Section: Introductionmentioning

confidence: 99%

Capsular Polysaccharide is a Main Component ofMycoplasma ovipneumoniaein the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

Jiang

Song

et al. 2017

Mediators of Inflammation

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Mycoplasma ovipneumoniae (M. ovipneumoniae) is characterized as an etiological agent of primary atypical pneumonia that specifically infects sheep and goat. In an attempt to better understand the pathogen-host interaction between the invading M. ovipneumoniae and airway epithelial cells, we investigated the host inflammatory responses against capsular polysaccharide (designated as CPS) of M. ovipneumoniae using sheep bronchial epithelial cells cultured in an air-liquid interface (ALI) model. Results showed that CPS derived from M. ovipneumoniae could activate toll-like receptor- (TLR-) mediated inflammatory responses, along with an elevated expression of nuclear factor kappa B (NF-κB), activator protein-1 (AP-1), and interferon regulatory factor 3 (IRF3) as well as various inflammatory-associated mediators, representatively including proinflammatory cytokines, such as IL1β, TNFα, and IL8, and anti-inflammatory cytokines such as IL10 and TGFβ of TLR signaling cascade. Mechanistically, the CPS-induced inflammation was TLR initiated and was mediated by activations of both MyD88-dependent and MyD88-independent signaling pathways. Of importance, a blockage of CPS with specific antibody led a significant reduction of M. ovipneumoniae-induced inflammatory responses in sheep bronchial epithelial cells. These results suggested that CPS is a key virulent component of M. ovipneumoniae, which may play a crucial role in the inflammatory response induced by M. ovipneumoniae infections.

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Expression of functions by normal sheep alveolar macrophages and their alteration by interaction with Mycoplasma ovipneumoniae

Cited by 22 publications

References 21 publications

Genome-Wide Histone Modifications and CTCF Enrichment Predict Gene Expression in Sheep Macrophages

Genome-Wide Histone Modifications and CTCF Enrichment Predict Gene Expression in Sheep Macrophages

Comparison of phenotypic and genotypic profiles among caprine and ovineMycoplasma ovipneumoniaestrains

Capsular Polysaccharide is a Main Component ofMycoplasma ovipneumoniaein the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

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