Experimental Contagious Caprine Pleuropneumonia: A Long Term Study on the Course of Infection and Pathology in a Flock of Goats Infected with Mycoplasma capricolum subsp. capripneumoniae

Wesonga, Hezron; Bölske, Göran; Thiaucourt, François; Wanjohi, C.; Lindberg, R.

doi:10.1186/1751-0147-45-167

Cited by 27 publications

(17 citation statements)

References 31 publications

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“…Our finding of CCPP gross lesions at postmortem which revealed lung exudate containing large clots of fibrin, adhesion of lungs to the thoracic wall, froth in the trachea, enlarged bronchial lymph nodes, and pneumonic lung tissues are similar with those of the previous study of Wesonga et al (2004) who reported the lesions of classical CCPP caused by Mccp . These observations are also matched with the findings of others (OIE 2008; Sadique et al 2012).…”

Section: Discussionsupporting

confidence: 91%

Epidemiological investigations of contagious caprine pleuropneumonia in selected districts of Borana zone, Southern Oromia, Ethiopia

Teshome¹,

Sori

Sacchini

et al. 2018

Trop Anim Health Prod

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From November 2016 to April 2017, a cross-sectional study to determine the sero-prevalence of contagious caprine pleuropneumonia (CCPP) and to investigate its epidemiology was conducted in selected districts of Borana zone in Ethiopia. In addition, the study aimed at identifying Mccp antigens using species specific primer of PCR. A multistage random sampling was implemented to select districts, pastoral associations (villages), and households. A total of 890 serum samples of small ruminants that had not been vaccinated (goats n = 789 and sheep n = 101) were collected and screened for the presence of antibodies against Mycoplasma capricolum subspecies capripneumoniae using a competitive enzyme-linked immunosorbent assay. Lung tissues and pleural fluid samples were collected from 3 sero-positive and clinically suspected goats for isolation of Mycoplasma capricolum subspecies capripneumoniae . Serology showed that overall 31.2% (246/789) of goats and 12.9% (13/101) of sheep were positive with statistically significant differences between districts ( p = 0.001). Multivariable logistic regression analysis revealed that goats from Moyale and Yabello districts had higher odds of being positive than goats from Elwoya district with odd ratios of 2.05 and 1.61, respectively. Age of goats was also significantly associated with sero-positivity (OR = 1.47; CI 95% 1.2–1.8). Mycoplasma capricolum subspecies capripneumoniae was identified in 6 (75%) of the tissue samples using species-specific primer of PCR. Besides improving the understanding of the epidemiology of CCPP in the selected districts and demonstrating its wide distribution, the study highly also provides evidence of the possible role of sheep in the maintenance of the disease.

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Section: Discussionsupporting

confidence: 91%

Epidemiological investigations of contagious caprine pleuropneumonia in selected districts of Borana zone, Southern Oromia, Ethiopia

Teshome¹,

Sori

Sacchini

et al. 2018

Trop Anim Health Prod

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“…The ranking of CCPP by farmers in Agago and Otuke as second among the health challenges of their goats corroborates with Southern Ethiopia participatory investigation of CCPP where farmers ranked the disease major constraint in goat production [11]. The clinical signs and postmortem lesions identified by farmers in Agago and Otuke were closely related to earlier documented clinical signs of the disease [2] [12].…”

Section: Discussionsupporting

confidence: 62%

A Survey for Contagious Caprine Pleuropneumonia in Agago and Otuke Districts in Northern Uganda

Atim¹,

Ayebazibwe²,

Mwiine³

et al. 2016

OJVM

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Background: Contagious Caprine Pleuropneumonia (CCPP) is a devastating disease of goats caused by Mycoplasma capricolum subsp. Capripneumoniae (Mccp). The disease was first confirmed in Uganda in 1995 in Karamoja region. Contagious Caprine Pleuropneumonia negatively impacts on goats' productivity but its extent and magnitude among the local communities in Uganda remain unknown. A cross sectional study was conducted in the districts of Agago and Otuke neighboring Karamoja in Northern Uganda during the months of July and August 2011 to explore for the status of the disease. Methods: Five hundred and four serum samples from goats were obtained from randomly selected unvaccinated herds and 100 goats from vaccinated herds. Serum samples were examined for antibodies against Mycoplasma capricolum subsp. Capripneumoniae (Mccp) by ELISA method. A total of 162 semi-structured questionnaires were administered to selected farmers to obtain information on their understanding of the disease and the risk factors they associated with CCPP. Eight focus group discussions were also conducted with selected farmer groups to obtain detailed qualitative information on CCPP. Results: Among the unvaccinated goats, seroprevalence of CCPP was 32 (17.7%) and 52 (23.3%) for Agago and Otuke districts respectively. Levels of antibodies against Mccp were higher among vaccinated goats than unvaccinated ones (mean optical densities (ODs) of 0.905 and 0.776, p = 0.08). Majority of the farmers 121 (74.7%) had knowledge on CCPP and recognized that CCPP was among the major challenges to goat production in Uganda. Conclusions: This study demonstrated that CCPP was prevalent in Agago and Otuke districts, which are outside but close to Karamoja region where the disease was previously confirmed. There is a need for wider and detailed studies to investigate further CCPP in other districts of Uganda for effective preventive and control of CCPP in Uganda and the neighboring countries.

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“…The development of an efficacious vaccine formula against CCPP requires a robust and reproducible experimental challenge model [9]. Past infection models for CCPP include in-contact challenge [10–12], endobronchial inoculation [3, 12, 13] and intratracheal administration [10]. Although mimicking the natural disease transmission, in-contact experiments are often time-consuming, require a large number of animals and are difficult to standardize.…”

Section: Introductionmentioning

confidence: 99%

“…Although mimicking the natural disease transmission, in-contact experiments are often time-consuming, require a large number of animals and are difficult to standardize. The latter two infection methods are technically challenging and can result in different pathomorphological outcomes ranging from the absence of any clinical or pathomorphological changes to severe clinical disease and pathology [10, 13]. Consequently, an improved model to reproduce CCPP would not only enable vaccine efficacy studies but also foster in vivo studies that provide insights regarding the molecular mechanisms associated with pathogenicity and the virulence traits involved.…”

Section: Introductionmentioning

confidence: 99%

Reproduction of contagious caprine pleuropneumonia reveals the ability of convalescent sera to reduce hydrogen peroxide production in vitro

Liljander

Sacchini

Stoffel

et al. 2019

Vet Res

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Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. capripneumoniae is a severe disease widespread in Africa and Asia. Limited knowledge is available on the pathogenesis of this organism, mainly due to the lack of a robust in vivo challenge model and the means to do site-directed mutagenesis. This work describes the establishment of a novel caprine challenge model for CCPP that resulted in 100% morbidity using a combination of repeated intranasal spray infection followed by a single transtracheal infection employing the recent Kenyan outbreak strain ILRI181. Diseased animals displayed CCPP-related pathology and the bacteria could subsequently be isolated from pleural exudates and lung tissues in concentrations of up to 109 bacteria per mL as well as in the trachea using immunohistochemistry. Reannotation of the genome sequence of ILRI181 and F38T revealed the existence of genes encoding the complete glycerol uptake and metabolic pathways involved in hydrogen peroxide (H2O2) production in the phylogenetically related pathogen M. mycoides subsp. mycoides. Furthermore, the expression of L-α-glycerophosphate oxidase (GlpO) in vivo was confirmed. In addition, the function of the glycerol metabolism was verified by measurement of production of H2O2 in medium containing physiological serum concentrations of glycerol. Peroxide production could be inhibited with serum from convalescent animals. These results will pave the way for a better understanding of host–pathogen interactions during CCPP and subsequent vaccine development.Electronic supplementary materialThe online version of this article (10.1186/s13567-019-0628-0) contains supplementary material, which is available to authorized users.

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Experimental Contagious Caprine Pleuropneumonia: A Long Term Study on the Course of Infection and Pathology in a Flock of Goats Infected with Mycoplasma capricolum subsp. capripneumoniae

Cited by 27 publications

References 31 publications

Epidemiological investigations of contagious caprine pleuropneumonia in selected districts of Borana zone, Southern Oromia, Ethiopia

Epidemiological investigations of contagious caprine pleuropneumonia in selected districts of Borana zone, Southern Oromia, Ethiopia

A Survey for Contagious Caprine Pleuropneumonia in Agago and Otuke Districts in Northern Uganda

Reproduction of contagious caprine pleuropneumonia reveals the ability of convalescent sera to reduce hydrogen peroxide production in vitro

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