Mycoplasma bovis MBOV_RS02825 Encodes a Secretory Nuclease Associated with Cytotoxicity

Zhang, Hui; Zhao, Gang; Guo, Yusi; Menghwar, Harish; Chen, Yingyu; Chen, Huanchun; Guo, Aizhen

doi:10.3390/ijms17050628

Cited by 48 publications

(57 citation statements)

References 50 publications

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“…In vitro studies using transposon mutants disclosed MBOVPG45_0215 as exhibiting the majority of nuclease activity for the DNA substrates tested (Sharma, Tivendale, Markham, & Browning, ). A recombinant form of a second nuclease MBOV_RS02825 (equivalent to MBOVPG45_0310) was shown to degrade the DNA component of NETs and to induce apoptosis of macrophages (Zhang et al., ). The gene encoding this nuclease resides within an operon of genes that likely mediates nucleotide binding and uptake, suggesting a role in nucleotide acquisition also.…”

Section: The Causative Organismmentioning

confidence: 99%

Gap analysis ofMycoplasma bovisdisease, diagnosis and control: An aid to identify future development requirements

Calcutt

Lysnyansky

Sachse

et al. 2018

Transbound Emerg Dis

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There is a worldwide problem of disease caused by Mycoplasma (M.) bovis in cattle; it has a significant detrimental economic and animal welfare impact on cattle rearing. Infection can manifest as a plethora of clinical signs including mastitis, pneumonia, arthritis, keratoconjunctivitis, otitis media and genital disorders that may result in infertility and abortion. Current diagnosis and control information are reviewed and analysed to identify gaps in knowledge of the causative organism in respect of the disease pathology, diagnosis and control methods. The main considerations are as follows: no vaccines are commercially available; antimicrobial resistance is increasing; diagnostic and antimicrobial sensitivity testing needs to be improved; and a pen-side test would facilitate more rapid diagnosis and implementation of treatment with antimicrobials. More data on host susceptibility, stress factors, immune response and infectious dose levels are required. The impact of asymptomatic carriers, M. bovis survival in the environment and the role of wildlife in transmitting the disease also needs investigation. To facilitate development of vaccines, further analysis of more M. bovis genomes, its pathogenic mechanisms, including variable surface proteins, is required, along with reproducible disease models.

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Section: The Causative Organismmentioning

confidence: 99%

Gap analysis ofMycoplasma bovisdisease, diagnosis and control: An aid to identify future development requirements

Calcutt

Lysnyansky

Sachse

et al. 2018

Transbound Emerg Dis

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“…Several M. bovis HB0801 proteins have previously been shown to be secreted proteins with significant functions. For example, MbovNase induces apoptosis [ 14 ]; P27 (MBOV_RS03440) is an immunogenic protein [ 19 ]; MbovP579 can be used as a diagnostic biomarker for M. bovis [ 13 ]; and NADH oxidase is an active enzyme that also functions as an adhesin [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, to date, few secretory proteins of M. bovis have been identified. However, preliminary studies have demonstrated that they play significant roles in inducing immunity and as diagnostic biomarkers [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

In Silico Identification of Novel Immunogenic Secreted Proteins of Mycoplasma bovis from Secretome Data and Experimental Verification

et al. 2020

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Mycoplasma bovis is a major pathogen, responsible for bovine respiratory diseases worldwide. The present lack of effective control measures leaves cattle owners at considerable perpetual risk of M. bovis outbreaks. In this study, we identified M. bovis secreted immunogenic proteins in silico as potential candidates for novel diagnostic agents and vaccines. We used immunoinformatics to analyze 438 M. bovis proteins previously identified with a label-free proteomics analysis of virulent M. bovis HB0801 (P1) and its attenuated P150 strains. The subcellular localization of these proteins was preliminarily screened and 59 proteins were found to be secreted extracellular proteins. Twenty-seven of these proteins contained a large number of predictive T-cell epitopes presented by major histocompatibility complex (MHC) class I and II molecules. Twenty-two of these 27 proteins had a high number of conformational B-cell epitopes, predicted from the corresponding 3D structural templates, including one unique to P1, two unique to P150, and 19 common to both strains. Five proteins were selected for further validation, and two of these, MbovP274 and MbovP570, were successfully expressed and purified. Both were confirmed to be secretory and highly immunogenic proteins that induced a mouse antibody response, reacted with cattle serum positive for M. bovis infection, and significantly increased the production of interleukin 8 (IL-8), IL-12 and interferon γ (IFN-γ) during the secretion of these three cytokines by both M. bovis mutants of these genes. These results should be useful in the development of novel immunological agents against M. bovis infection.

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“…In M . bovis , it was shown to function as cytotoxic, secreted nuclease with the potential to induce apoptosis in macrophages [ 46 ]. In M .…”

Section: Discussionmentioning

confidence: 99%

Validation of a novel Mho microarray for a comprehensive characterisation of the Mycoplasma hominis action in HeLa cell infection

et al. 2017

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Mycoplasma hominis is the second smallest facultative pathogen of the human urogenital tract. With less than 600 protein-encoding genes, it represents an ideal model organism for the study of host-pathogen interactions. For a comprehensive characterisation of the M. hominis action in infection a customized Mho microarray, which was based on two genome sequences (PG21 and LBD-4), was designed to analyze the dynamics of the mycoplasma transcriptome during infection and validated for M. hominis strain FBG. RNA preparation was evaluated and adapted to ensure the highest recovery of mycoplasmal mRNAs from in vitro HeLa cell infection assays. Following cRNA hybridization, the read-out strategy of the hybridization results was optimized and confirmed by RT-PCR. A statistically robust infection assay with M. hominis strain FBG enabled the identification of differentially regulated key effector molecules such as critical cytoadhesins (4 h post infection (pI)), invasins (48 h pI) and proteins associated with establishing chronic infection of the host (336 h pI). Of the 294 differentially regulated genes (>2-fold) 128 (43.5%) encoded hypothetical proteins, including lipoproteins that seem to play a central role as virulence factors at each stage of infection: P75 as a novel cytoadhesin candidate, which is also differentially upregulated in chronic infection; the MHO_2100 protein, a postulated invasin and the MHO_730-protein, a novel ecto-nuclease and domain of an ABC transporter, the function of which in chronic infection has still to be elucidated. Implementation of the M. hominis microarray strategy led to a comprehensive identification of to date unknown candidates for virulence factors at relevant stages of host cell infection.

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Mycoplasma bovis MBOV_RS02825 Encodes a Secretory Nuclease Associated with Cytotoxicity

Cited by 48 publications

References 50 publications

Gap analysis ofMycoplasma bovisdisease, diagnosis and control: An aid to identify future development requirements

Gap analysis ofMycoplasma bovisdisease, diagnosis and control: An aid to identify future development requirements

In Silico Identification of Novel Immunogenic Secreted Proteins of Mycoplasma bovis from Secretome Data and Experimental Verification

Validation of a novel Mho microarray for a comprehensive characterisation of the Mycoplasma hominis action in HeLa cell infection

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