Validation of a novel Mho microarray for a comprehensive characterisation of the Mycoplasma hominis action in HeLa cell infection

Henrich, Birgit; Kretzmer, Freya; Deenen, René; Köhrer, Karl

doi:10.1371/journal.pone.0181383

Cited by 12 publications

(10 citation statements)

References 54 publications

(73 reference statements)

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“…During adhesion, human cells first interact with the plasma membrane of these microorganisms, specifically with their lipoproteins. Recently, cytoadhesion and invasion present on the membrane of M. hominis were shown to interact with HeLa cells [ 48 ]. Hopfe et al [ 49 ] showed that, after 4 h of infection of HeLa cells with M. hominis , genes related to the cell cycle, growth, and cell death are highly regulated, and lipoproteins are generally responsible for inducing inflammation, apoptosis, and cell death [ 5 , 50 ].…”

Section: Resultsmentioning

confidence: 99%

Potential of Maintaining a Healthy Vaginal Environment by TwoLactobacillusStrains Isolated from Cocoa Fermentation

Melgaço

Pessoa

Freire

et al. 2018

BioMed Research International

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Bacteria in the genera Mycoplasma and Ureaplasma do not have cell walls and therefore interact with host cells through lipid-associated membrane proteins (LAMP). These lipoproteins are important for both surface adhesion and modulation of host immune responses. Mycoplasma and Ureaplasma have been implicated in cases of bacterial vaginosis (BV), which can cause infertility, abortion, and premature delivery. In contrast, bacteria of the genus Lactobacillus, which are present in the vaginal microbiota of healthy women, are thought to inhibit local colonization by pathogenic microorganisms. The aim of the present study was to evaluate the in vitro interactions between lipoproteins of Mycoplasma and Ureaplasma species and vaginal lineage (HMVII) cells and to study the effect of Lactobacillus isolates from cocoa fermentation on these interactions. The tested Lactobacillus strains showed some important probiotic characteristics, with autoaggregation percentages of 28.55% and 31.82% for L. fermentum FA4 and L. plantarum PA3 strains, respectively, and percent adhesion values of 31.66 and 41.65%, respectively. The two strains were hydrophobic, with moderate to high hydrophobicity values, 65.33% and 71.12% for L. fermentum FA4 and L. plantarum PA3 in toluene. Both strains secreted acids into the culture medium with pH=4.32 and pH=4.33, respectively, and showed antibiotics susceptibility profiles similar to those of other lactobacilli. The strains were also able to inhibit the death of vaginal epithelial cells after incubation with U. parvum LAMP from 41.03% to 2.43% (L. fermentum FA4) and 0.43% (L. plantarum PA3) and also managed to significantly decrease the rate of cell death caused by the interaction with LAMP of M. hominis from 34.29% to 14.06% (L. fermentum FA4) and 14.61% (L. plantarum PA3), thus demonstrating their potential for maintaining a healthy vaginal environment.

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Section: Resultsmentioning

confidence: 99%

Potential of Maintaining a Healthy Vaginal Environment by TwoLactobacillusStrains Isolated from Cocoa Fermentation

Melgaço

Pessoa

Freire

et al. 2018

BioMed Research International

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“…Mutants of interest were isolated over the course of our transformation experiments, such as mutant 28-2, which harbors the tet (M)-carrying transposon within the P75 lipoprotein gene that is potentially involved in the cytoadherence of M . hominis 34,35 . After verifying that the gene was knocked-out by PCR and RT-PCR, the adhesion ability of the mutant strain was tested using HeLa cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

Random transposon insertion in the Mycoplasma hominis minimal genome

Rideau

Roy

Sagné

et al. 2019

Sci Rep

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Mycoplasma hominis is an opportunistic human pathogen associated with genital and neonatal infections. Until this study, the lack of a reliable transformation method for the genetic manipulation of M. hominis hindered the investigation of the pathogenicity and the peculiar arginine-based metabolism of this bacterium. A genomic analysis of 20 different M. hominis strains revealed a number of putative restriction-modification systems in this species. Despite the presence of these systems, a reproducible polyethylene glycol (PEG)-mediated transformation protocol was successfully developed in this study for three different strains: two clinical isolates and the M132 reference strain. Transformants were generated by transposon mutagenesis with an efficiency of approximately 10−9 transformants/cell/µg plasmid and were shown to carry single or multiple mini-transposons randomly inserted within their genomes. One M132-mutant was observed to carry a single-copy transposon inserted within the gene encoding P75, a protein potentially involved in adhesion. However, no difference in adhesion was observed in cell-assays between this mutant and the M132 parent strain. Whole genome sequencing of mutants carrying multiple copies of the transposon further revealed the occurrence of genomic rearrangements. Overall, this is the first time that genetically modified strains of M. hominis have been obtained by random mutagenesis using a mini-transposon conferring resistance to tetracycline.

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“…In addition, Henrich et. al characterized several M. hominis genes involved in HeLa cells intracellular infection [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Trichomonas vaginalis Transports Virulent Mycoplasma hominis and Transmits the Infection to Human Cells after Metronidazole Treatment: A Potential Role in Bacterial Invasion of Fetal Membranes and Amniotic Fluid

Thu

Margarita

Cocco

et al. 2018

Journal of Pregnancy

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Mycoplasma hominis is considered an opportunistic pathogen able to colonize the lower urogenital tract; in females the infection is associated with severe pregnancy and postpartum complications, including abortion, endometritis, preterm delivery, and low birth weight. Molecular mechanisms of pathogenicity and virulence effectors remain poorly characterized. A number of studies in the last decade have demonstrated that M. hominis can establish an endosymbiotic relationship with Trichomonas vaginalis, a urogenital parasitic protozoon, also associated with adverse pregnancy outcomes. Recently, two bacterial genes (alr and goiB) associated with amniotic cavity invasion and a single gene (goiC) associated with intra-amniotic infections and high risk of preterm delivery have been identified in M. hominis isolated from a group of pregnant patients. In this work we demonstrate that a high number of M. hominis intracellularly associated with T. vaginalis have goiC gene, in association with alr and goiB. In addition, we demonstrate that metronidazole treatment of M. hominis-infected T. vaginalis allows delivering viable intracellular goiC positive M. hominis from antibiotic-killed protozoa and that free M. hominis can infect human cell cultures. Results suggest that molecular diagnostic strategies to identify both pathogens and their virulence genes should be adopted to prevent severe complications during pregnancy.

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Validation of a novel Mho microarray for a comprehensive characterisation of the Mycoplasma hominis action in HeLa cell infection

Cited by 12 publications

References 54 publications

Potential of Maintaining a Healthy Vaginal Environment by TwoLactobacillusStrains Isolated from Cocoa Fermentation

Potential of Maintaining a Healthy Vaginal Environment by TwoLactobacillusStrains Isolated from Cocoa Fermentation

Random transposon insertion in the Mycoplasma hominis minimal genome

Trichomonas vaginalis Transports Virulent Mycoplasma hominis and Transmits the Infection to Human Cells after Metronidazole Treatment: A Potential Role in Bacterial Invasion of Fetal Membranes and Amniotic Fluid

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