Mx expression in Atlantic salmon (Salmo salar L.) parr in response to Listonella anguillarum bacterin, lipopolysaccharide and chromosomal DNA

Mx expression in gilthead sea bream (Sparus aurata L.) in response to poly I:C, bacterial LPS and chromosomal DNA: Preliminary study

“…1). This agrees with the results obtained with Senegalese sole [20], but not with those showed for Atlantic salmon [12], where a peak was obtained at 6 d and 9 d post-inoculation depending on the temperature. Other authors found a peak at 2 d [19], and at 24 h [21].…”

“…In vitro, LPS stimulates phagocytosis and the production of superoxide anions in Atlantic salmon macrophages, the production of the macrophage-activating factor in goldfish lymphocytes, the production of interleukin I-like molecules in catfish monocytes [9] and elicits the expression of cytokines such as tumour necrosis factor (TNF-a) in rainbow trout [10] and Atlantic salmon [11]. The ability of LPS to induce Mx responses has been demonstrated in fish [12].…”

“…In this study was used poly I:C, which has been extensively shown to induce in vitro and in vivo antiviral activities in fish [12,16e20] via the stimulation of IFN, LPS and DNA following previous results [12] but using V. alginolyticus, a common bacterium in the marine waters of the Canary Islands, where this study was carried out.…”

Cloning of an orange-spotted grouper (Epinephelus coioides) Mx cDNA and characterisation of its expression in response to nodavirus

“…Nevertheless, synthetic oligodeoxynucleotides containing CpG motifs have been shown to induce short-term expression of Mx mRNA in Atlantic salmon and to provide a degree of protection against challenge with IPNV [40]. This is the first report that the induction of Mx expression in Atlantic salmon parr was investigated following injection of vibrio bacterin, composed of Vibrio anguillarum serotypes I and II-ordalii, at two different temperatures in comparison with the potent Mx inducer poly[I:C] [41,42]. Some of study aimed to identify substances which are already known to be immunostimulants in fish as being stimulatory for Mx.…”

Isolation of a novel fish thymidylate kinase gene, upregulated in Atlantic salmon (Salmo salar L.) following infection with the monogenean parasite Gyrodactylus salaris

“…LPS was generated from Listonella anguillarum serotype O1 (formerly Vibrio anguillarum) as described in [29] and dissolved in phosphate buffered saline (PBS) at 1 mg ml À1 . Poly I:C (Sigma) was dissolved in PBS to a final concentration of 5 mg ml À1 .…”