Isolation of a novel fish thymidylate kinase gene, upregulated in Atlantic salmon (Salmo salar L.) following infection with the monogenean parasite Gyrodactylus salaris

Collins, Catherine; Olstad, Kjetil; Sterud, Erik; Jones, Catherine S.; Noble, Leslie R.; Mo, Tor Atle; Cunningham, Carey O.

doi:10.1016/j.fsi.2007.03.001

Cited by 14 publications

(10 citation statements)

References 40 publications

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“…The number and size of mitochondria in macrophages were greatly increased after LPS induction (54). In other tissues, TYKi was also up-regulated by several inflammatory response-related cytokines (such as TNF␣, IFN␥, IL-1␤, and IFN␣) and infections of parasites (31,33,55). All these expression profiles suggest that UMP-CMPK2 is actively involved in macrophage activation and the inflammatory response.…”

Section: Discussionmentioning

confidence: 91%

Human UMP-CMP Kinase 2, a Novel Nucleoside Monophosphate Kinase Localized in Mitochondria

Johansson

Karlsson

2008

Journal of Biological Chemistry

107

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Enzyme deficiency in the salvage pathway of deoxyribonucleotide synthesis in mitochondria can cause mtDNA depletion syndromes. We have identified a human mitochondrial UMP-CMP kinase (UMP-CMPK, cytidylate kinase; EC 2.7.4.14), designated as UMP-CMP kinase 2 (UMP-CMPK2). The C-terminal domain of this 449-amino acid protein contains all consensus motifs of a nucleoside monophosphate kinase. Phylogenetic analysis showed that UMP-CMPK2 belonged to a novel nucleoside monophosphate kinase family, which was closer to thymidylate kinase than to cytosolic UMP-CMP kinase. Subcellular localization with green fluorescent protein fusion proteins illustrated that UMP-CMPK2 was localized in the mitochondria of HeLa cells and that the mitochondrial targeting signal was included in the N-terminal 22 amino acids. The enzyme was able to phosphorylate dUMP, dCMP, CMP, and UMP with ATP as phosphate donor, but the kinetic properties were different compared with the cytosolic UMP-CMPK. Its efficacy to convert dUMP was highest, followed by dCMP, whereas CMP and UMP were the poorest substrates. It also phosphorylated the monophosphate forms of the nucleoside analogs ddC, dFdC, araC, BVDU, and FdUrd, which suggests that UMP-CMPK2 may be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs. UMP-CMPK2 mRNA expression was exclusively detected in chronic myelogenous leukemia K-562 and lymphoblastic leukemia MOLT-4 among eight studied cancer cell lines. Particular high expression in leukemia cells, dominant expression in bone marrow, and tight correlation with macrophage activation and inflammatory response suggest that UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis.

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Section: Discussionmentioning

confidence: 91%

Human UMP-CMP Kinase 2, a Novel Nucleoside Monophosphate Kinase Localized in Mitochondria

Johansson

Karlsson

2008

Journal of Biological Chemistry

107

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“…The three-spined stickleback is the only host on which this parasite feeds and reproduces. Several studies have reported the impact of Gyrodactylus species on host immune system activation (Buchmann and Lindenstrøm 2002;Lindenstrom et al 2004;Collins et al 2007). Gyrodactylus infection has also been correlated with carotenoid input-the source of various male secondary sexual trait colourations (Kolluru et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Parasite diversity, patterns of MHC II variation and olfactory based mate choice in diverging three-spined stickleback ecotypes

et al. 2010

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Ecological speciation has been the subject of intense research in evolutionary biology but the genetic basis of the actual mechanism driving reproductive isolation has rarely been identified. The extreme polymorphism of the major histocompatibility complex (MHC), probably maintained by parasite-mediated selection, has been proposed as a potential driver of population divergence. We performed an integrative field and experimental study using three-spined stickleback river and lake ecotypes. We characterized their parasite load and variation at MHC class II loci. Fish from lakes and rivers harbor contrasting parasite communities and populations possess different MHC allele pools that could be the result of a combined action of genetic drift and parasite-mediated selection. We show that individual MHC class II diversity varies among populations and is lower in river ecotypes. Our results suggest the action of homogenizing selection within habitat type and diverging selection between habitat types. Finally, reproductive isolation was suggested by experimental evidence: in a flow channel design females preferred assortatively the odor of their sympatric male. This demonstrates the role of olfactory cues in maintaining reproductive isolation between diverging fish ecotypes

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“…Several studies have reported the impact of Gyrodactylus species on host immune system activation (Buchmann & Lindenstrøm 2002; Lindenstrom et al . 2004; Collins et al . 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The three-spined stickleback is the only host on which this parasite feeds and reproduces. Several studies have reported the impact of Gyrodactylus species on host immune system activation (Buchmann & Lindenstrøm 2002;Lindenstrom et al 2004;Collins et al 2007). Gyrodactylus infection has been correlated with carotenoid input -the source of various male secondary sexual trait colourations (Kolluru et al 2006).…”

Section: Discussionmentioning

confidence: 99%

MHC‐based mate choice combines good genes and maintenance of MHC polymorphism

et al. 2009

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Polymorphic genes of the major histocompatibility complex (MHC) are regarded as essential genes for individual fitness under conditions of natural and sexual selection. To test this hypothesis, we investigated the ultimate individual fitness trait--that of reproductive success. We used three-spined sticklebacks (Gasterosteus aculeatus) in seminatural enclosures, located in natural breeding areas where the experimental fish had been caught. During their reproductive period, fish were exposed continuously to their natural sympatric parasites. By genotyping almost 4000 eggs with nine microsatellites, we determined parenthood and inferred female mating decision. We found that with reference to their own MHC profile, female sticklebacks preferred to mate with males sharing an intermediate MHC diversity. In addition, males with a specific MHC haplotype were bigger and better at fighting a common parasite (Gyrodactylus sp.). This translated directly into Darwinian fitness since fish harbouring this specific MHC haplotype were more likely to be chosen and had a higher reproductive output. We conclude that females also based their mating decision on a specific MHC haplotype conferring resistance against a common parasite. This identifies and supports 'good genes'. We argue that such an interaction between host and parasite driving assortative mating is not only a prerequisite for negative frequency-dependent selection--a potential mechanism to explain the maintenance of MHC polymorphism, but also potentially speciation.

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Isolation of a novel fish thymidylate kinase gene, upregulated in Atlantic salmon (Salmo salar L.) following infection with the monogenean parasite Gyrodactylus salaris

Cited by 14 publications

References 40 publications

Human UMP-CMP Kinase 2, a Novel Nucleoside Monophosphate Kinase Localized in Mitochondria

Human UMP-CMP Kinase 2, a Novel Nucleoside Monophosphate Kinase Localized in Mitochondria

Parasite diversity, patterns of MHC II variation and olfactory based mate choice in diverging three-spined stickleback ecotypes

MHC‐based mate choice combines good genes and maintenance of MHC polymorphism

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