Mutation Spectrum in Dendranthema Grandiflora Tzvelev After in Vivo and in Vitro Regeneration of Plants From Irradiated Leaves

Zalewska, M.; Jerzy, M.

doi:10.17660/actahortic.1997.447.122

Cited by 9 publications

(4 citation statements)

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“…Tissue culture studies on chrysanthemum were first started in 1952 by Morel and Martin (3). Adventitious in vitro shoot regeneration in chrysanthemum is the most commonly used technique for producing new cultivars, either in mutation breeding (4) or in genetic transformation (5, 6). Formation of the new organs is a distinctive characteristic of the plant regeneration (7).…”

Section: Introductionmentioning

confidence: 99%

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

et al. 2018

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BackgroundChrysanthemum; also commonly known as mums or chrysanths, is one of the most important ornamental crops worldwide. Introducing desirable traits into this valuable plant by the conventional breeding has so far been faced with some restrictions due to the limited gene pool and cross-incompatibility. Therefore, breeders have decided to exploit Agrobacterium-mediated transformation methods in order to satisfy the growing market demands. However, more efficient in vitro regeneration protocols are required for this approach.ObjectivesThe objective of this research was to develop an efficient protocol for an in vitro plant regeneration by the examining the effects of various combinations and concentrations of the plant growth regulators (PGRs) and different explants types.Materials and MethodsThe leaf and petiole explants of the Chrysanthemum morifolium cv. ‘Resomee Splendid’ were collected from the in vitro grown plantlets. Murashige and Skooge (MS) medium was supplemented with different concentrations and combinations of benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ). Thereafter, the effects of these hormonal treatments were investigated on shoot initiation percentage, the average number of shoots per explants, callogenesis, and the type of organogenesis in regard to both types of the explants.ResultsShoots were directly formed from leaf explants on the media that only contained BAP without callus formation. Amongst the other hormonal treatments, a combination of 4.5 mg.L-1 BAP plus 1 mg.L-1 NAA resulted in the direct organogenesis from the leaf explants, which was superior to the other combinations and concentrations. In regard to the petiole explants, direct shoot formation occurred in all the media except for the ones which were fortified with TDZ. In this case, considering the shoot initiation percentage and the mean shoot number per explants, the best results were achieved in the medium supplemented with 1.5 mg.L-1 BAP and 1 mg.L-1 NAA. Results showed that interaction of either BAP or TDZ with NAA was necessary for the callus induction.ConclusionsSignificant differences in shoot initiation percentage and the average number of shoots per explants were observed both in leaves and petioles grown on different media. Moreover, the callogenesis rates, as well as organogenesis types, showed some differences among the studied explants when compared on the same media.

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Section: Introductionmentioning

confidence: 99%

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

et al. 2018

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“…Irradiasi sinar gamma pada krisan sudah banyak dilakukan dan menghasilkan banyak varietas baru (Zalewska & Jerzy, 1996;Jerzy & Zalewska, 1997;Datta, 2001;Zalewska et al, 2011). Menurut Rout et al (2006) mutasi dapat digunakan untuk meningkatkan nilai tanaman, termasuk warna, ukuran dan bentuk bunga, serta kualitas produk.…”

Section: Abstrakunclassified

Variabilitas Genotipe-Genotipe Mutan Krisan (Dendranthema grandiflora Tzvelv.) Generasi MV5 Hasil Irradiasi Sinar Gamma

Kurniasih¹,

Ruswandi

Karmana

et al. 2016

Agrikultura

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Variability of mutant genotypes chrysanthemum (Dendranthema grandiflora Tzvelv.) fifth generationsthrough gamma iIrradiationDendranthema grandiflorum Tzvelv. is a major floriculture in Indonesia, and it is one of the five most popular flowers in Indonesia. Chrysanthemum varieties in Indonesia is largely the introduced varieties. Chrysanthemum hybridization especially for decorative flower type in order to obtain superior varieties is relatively difficult, so the mutation breeding is one approach that can be taken to get the chrysanthemum varieties with different phenotypic performances with the that parent.The purpose of this study was to obtain information genetic and phenotypic variability characters observed on chrysanthemum irradiated with gamma ray. The experiment was conducted by an experimental method using a randomized block design (RBD). The treatments consisted of 37 mutants genotypes and 11 genotypes chrysanthemums parent as controls with two replications. The results of this study indicated that the genotypes tested had broad genetic and phenotipic variation for the plant height, flower diameter, number of flower and neck lengths.Key words: Chrysanthemum mutants, Variability, Gamma ray irradiation.ABSTRAKKrisan merupakan komoditas tanaman hias utama di Indonesia dan paling banyak diminati masyarakat. Varietas-varietas krisan yang beredar di Indonesia sebagian besar merupakan varietas introduksi. Persilangan krisan khususnya untuk tipe bunga dekoratif dalam rangka memperoleh varietas unggul relatif sulit dilakukan, sehingga pemuliaan mutasi merupakan salah satu pendekatan yang dapat ditempuh untuk mendapatkan varietas krisan dengan penampilan fenotipik yang berbeda dengan induknya. Tujuan penelitian ini adalah untuk mendapatkan informasi variabilitas genetik dan fenotipik karakter-karakter yang diamati pada tanaman krisan yangd iradiasi dengan sinar gamma. Percobaan dilakukan dengan metode eksperimen menggunakan Rancangan Acak Kelompok (RAK). Perlakuan terdiri atas 37 genotipe mutan krisan dan 11 genotipe tetua krisan sebagai kontrol dengan dua ulangan. Hasil penelitian menunjukkan bahwa genotipe-genotipe yang diuji memiliki variabilitas yang luas untuk karakter tinggi tanaman, diameter bunga, jumlah kuntum dan panjang tangkai bunga.Kata kunci: Mutan krisan, Variabilitas, Sinar gamma

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“…Adventitious shoot regeneration in chrysanthemum in vitro is important mostly in creating new cultivars, both in mutation breeding (Broertjes et al 1976;Jerzy and Zalewska 1996;Zalewska and Jerzy 1997) and in separating chimera components (Zalewska et al 2007), as well as in genetic transformation (Nahid et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Induction of adventitious shoot regeneration in chrysanthemum as affected by the season

Zalewska

Lema-Rumińska

Miler

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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The subject of investigation was Chrysanthemum x grandiflorum (Ramat.) Kitam., FSatinbleu_. Leaf explants and internodes were excised from the middle part of the donor sterile plant. Two methods of explant inoculation were applied: explants were placed polarly and horizontally. Modified MS medium (Murashige and Skoog 1962), supplemented with 11.4 μM indoleacetic acid and 2.7 μM 6-benzylaminopurine, were prepared to induce adventitious bud formation. Four dates of explant inoculation on the medium were tested: January 15, April 15, July 15, and October 15. Thus, regeneration occurred in winter, spring, summer, and autumn. In the present study, a more intensive regeneration in FSatinbleu_ chrysanthemum was observed in summer and autumn than in spring and winter, irrespective of the kind of explant and the inoculation method.

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Mutation Spectrum in Dendranthema Grandiflora Tzvelev After in Vivo and in Vitro Regeneration of Plants From Irradiated Leaves

Cited by 9 publications

References 0 publications

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

Variabilitas Genotipe-Genotipe Mutan Krisan (Dendranthema grandiflora Tzvelv.) Generasi MV5 Hasil Irradiasi Sinar Gamma

Induction of adventitious shoot regeneration in chrysanthemum as affected by the season

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