Induction of adventitious shoot regeneration in chrysanthemum as affected by the season

Zalewska, M.; Lema-Rumińska, Justyna; Miler, Natalia; Gruszka, Magdalena; Dąbal, Weronika

doi:10.1007/s11627-010-9330-7

Cited by 16 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kitam. ‘Satinbleu’, the greatest dynamic of the regeneration on leaves was observed between the 3rd and the 5th week of culture (18). Considering that regeneration mostly started at the cut sites, it can be concluded that wound stimuli might supply a primary inductive trigger for the mentioned phenomenon (7, 25, 26).…”

Section: Discussionmentioning

confidence: 99%

“…The maximum percentages of the shoot regeneration in addition to shoots per se per explants in the studied cultivar were achieved on a medium containing equal concentrations (0.5 mg.L -1 ) of the NAA and BA (15). Also, The impact of various types of explants on regeneration has been studied in prior investigations, such as petal (5, 16, 17), leaf (17, 18), stem (17), and petiole (6, 17). The efficiency of the leaf, shoot tip, and ray floret explants of the chrysanthemum for the direct in vitro regeneration were compared in a survey and shoot tips were reported to be the most suitable types of explants (19).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

et al. 2018

View full text Add to dashboard Cite

BackgroundChrysanthemum; also commonly known as mums or chrysanths, is one of the most important ornamental crops worldwide. Introducing desirable traits into this valuable plant by the conventional breeding has so far been faced with some restrictions due to the limited gene pool and cross-incompatibility. Therefore, breeders have decided to exploit Agrobacterium-mediated transformation methods in order to satisfy the growing market demands. However, more efficient in vitro regeneration protocols are required for this approach.ObjectivesThe objective of this research was to develop an efficient protocol for an in vitro plant regeneration by the examining the effects of various combinations and concentrations of the plant growth regulators (PGRs) and different explants types.Materials and MethodsThe leaf and petiole explants of the Chrysanthemum morifolium cv. ‘Resomee Splendid’ were collected from the in vitro grown plantlets. Murashige and Skooge (MS) medium was supplemented with different concentrations and combinations of benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ). Thereafter, the effects of these hormonal treatments were investigated on shoot initiation percentage, the average number of shoots per explants, callogenesis, and the type of organogenesis in regard to both types of the explants.ResultsShoots were directly formed from leaf explants on the media that only contained BAP without callus formation. Amongst the other hormonal treatments, a combination of 4.5 mg.L-1 BAP plus 1 mg.L-1 NAA resulted in the direct organogenesis from the leaf explants, which was superior to the other combinations and concentrations. In regard to the petiole explants, direct shoot formation occurred in all the media except for the ones which were fortified with TDZ. In this case, considering the shoot initiation percentage and the mean shoot number per explants, the best results were achieved in the medium supplemented with 1.5 mg.L-1 BAP and 1 mg.L-1 NAA. Results showed that interaction of either BAP or TDZ with NAA was necessary for the callus induction.ConclusionsSignificant differences in shoot initiation percentage and the average number of shoots per explants were observed both in leaves and petioles grown on different media. Moreover, the callogenesis rates, as well as organogenesis types, showed some differences among the studied explants when compared on the same media.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

et al. 2018

View full text Add to dashboard Cite

show abstract

“…In barley, Sharma et al (2005) observed such a variation when donor plants were grown in the growth chambers. In petal cultures of chrysanthemum, the seasonal effect on shoot regeneration appeared when donor plants were kept exclusively in vitro (Zalewska et al 2011) or in an environmentally controlled greenhouse (Naing and Kim 2014). Various periods of honey storage have rather little effect on our results.…”

Section: Discussionmentioning

confidence: 62%

Improved plant regeneration in callus cultures of Sorghum bicolor (L.) Moench

Dreger

Mól

Deja

et al. 2019

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Sorghum bicolor is a recalcitrant species for tissue culture regeneration and genetic transformation. Browning of explants is one of the factors limiting organ and tissue cultures. To overcome this, callus tissue was initiated from the shoot tips of in vitro germinating seeds (S. bicolor cv. Róna 1), and then cultured on modified MS media (Murashige and Skoog in Physiol Plant 15:473-497, 1962). In the first experiment, we tested callus induction on several media supplemented with casein hydrolysate, polyvinylpyrrolidone, honey, and sucrose. The best callus induction was recorded for the medium with honey and sucrose (80.0%) and for control medium (79.8%). Shoot regeneration was tested on the MS medium with 6-benzylaminopurine (BAP) supplemented with honey and sucrose at a 1:1 ratio (by weight) or with sucrose only. The highest percentage of calluses regenerating shoots was noted for those induced on the medium with sucrose and honey-approx. four times higher when compared to the control. Rooted plantlets were acclimatized with a 92% survival rate. In the second experiment, we analyzed culture responses to various ways of honey application to the induction media: honey (autoclaved or filtered) in presence or absence of sucrose. Supplementation of the medium with fructose, glucose, and maltose at a proportion typical for honey was also investigated. The explant and callus survival rates were similar to those of the honey-sucrose combination in the first experiment. Only presence of both sucrose and honey in the induction medium improved the total regeneration rate to 37.9% over the control (18.8%). Sucrose and honey appear to act synergistically for shoot regeneration in callus cultures of sorghum.

show abstract

“…It is commonly regarded as one of the most effective micropropagation methods, facilitating the generation of a large number of plants in a very short time [Kulpa 2012]. Plant regeneration with chrysanthemum via somatic embryogenesis gives higher efficiency than adventitious organogenesis [Zalewska et al 2007]. Somatic embryogenesis in chrysanthemum is induced on MS medium [Murashige and Skoog 1962] with addition of auxin -2,4--dichlorophenoxyacetic acid (2,4-D) or α-naphthylacetic acid (NAA) as well as cytokinins: 6-benzylaminopurine (BAP) or kinetin (KIN), and generally this process occurs indirectly, via callus [Naing et al 2013, Lema--Rumińska and Śliwińska 2015.…”

Section: Introductionmentioning

confidence: 99%

ISSR markers in the genetic diversity of chrysanthemum plants derived via somatic embryogenesis

Michałowska

Lema-Rumińska

2018

Zeszyty Problemowe Postępów Nauk Rolniczych

Self Cite

View full text Add to dashboard Cite

show abstract

Induction of adventitious shoot regeneration in chrysanthemum as affected by the season

Cited by 16 publications

References 15 publications

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. ‘Resomee Splendid’

Improved plant regeneration in callus cultures of Sorghum bicolor (L.) Moench

ISSR markers in the genetic diversity of chrysanthemum plants derived via somatic embryogenesis

Contact Info

Product

Resources

About