Mutants with general growth rate advantages are the predominant morphological mutants to be isolated from the Quorn® production plant

Simpson, D. R.; Withers, Julie M.; Wiebe, Marilyn G.; Robson, Geoffrey D.; Trinci, Anthony P. J.

doi:10.1017/s0953756297004644

Cited by 10 publications

(2 citation statements)

References 20 publications

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“…In order to identify a medium that could ensure high and reliable levels of DON production, preliminary assays were conducted by filter‐sterilizing liquid cultures containing different nutrients (1·65 g L −1 ammonium sulphate, 0·75 g L −1 urea or 2·125 g L −1 sodium nitrate), glucose (10 g L −1 ) and 20 mL L −1 nitrogen‐free modified Vogel's medium (Simpson et al ., 1998). Autoclaved potato dextrose broth (PDB, Difco, West Molesey, Surrey, UK) was also included as a complex medium reference.…”

Section: Methodsmentioning

confidence: 99%

Repression of deoxynivalenol accumulation and expression ofTrigenes inFusarium culmorumby fungicidesin vitro

Covarelli

Nicholson

2004

Plant Pathology

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A defined medium was developed in which to monitor deoxynivalenol (DON) accumulation, fungal growth and expression of genes involved in trichothecene biosynthesis (designated Tri genes). In liquid culture, DON accumulated shortly after maximum expression of Tri6 and coincident with expression of Tri5. This was generally 96 h after inoculation. The effects of sublethal concentrations of the fungicides azoxystrobin, trifloxystrobin, kresoxim-methyl and tebuconazole on biosynthesis of the trichothecene DON by Fusarium culmorum were studied using this medium. The strobilurin fungicides trifloxystrobin and azoxystrobin significantly reduced the accumulation of DON in culture medium at a range of concentrations. Kresoxim-methyl, also a strobilurin, and tebuconazole, a triazole, did not significantly reduce the accumulation of DON, although levels were lower than those in nonamended cultures. Trifloxystrobin significantly reduced the accumulation of DON when added to cultures before initiation of trichothecene biosynthesis. RT-PCR assays of the expression of Tri6 and Tri5 genes indicated that trifloxystrobin acted by inhibiting the initiation of trichothecene biosynthesis.

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Section: Methodsmentioning

confidence: 99%

Repression of deoxynivalenol accumulation and expression ofTrigenes inFusarium culmorumby fungicidesin vitro

Covarelli

Nicholson

2004

Plant Pathology

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“…These morphological mutants necessitate the termination of production fermentations, and thus their appearance results in a loss in productivity [3,2]. Colonial mutants have been isolated from turbidostat cultures [3,5] and from glucose-, magnesium-and nitrogen-limited chemostat cultures grown at high dilution rates (D = 0.14^0.19 h 3I ) [4,6]. They have not been observed in slow dilution rate (D = 0.05 h 3I ) glucose-limited chemostats and their appearance has been delayed by altering the culture pH or by imposing oscillations in the culture pH [7].…”

Section: Introductionmentioning

confidence: 99%

Peptone changes the timing and accumulation of morphological mutants of the Quorn® myco-protein fungus Fusarium graminearum A3/5 in glucose-limited chemostat cultures

Wiebe¹

1998

FEMS Microbiology Letters

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Fusarium graminearum A3/5 was grown at pH 5.8 and a dilution rate of 0.19 h−1 in glucose‐limited chemostat culture supplemented with mycological peptone for 528 h. Although the population changed (i.e. displayed periodic selection) at the same rate in supplemented (132±23 h) and unsupplemented cultures (124±12 h), highly branched mutants were detected much earlier in the former cultures than in the latter, although they failed to displace the sparsely branched parental population. It was demonstrated that the selective advantage (compared to A3/5) of some mutants isolated from unsupplemented glucose‐limited culture could be eliminated by addition of peptone to the medium, but that a general growth rate mutant retained its advantage in the peptone‐supplemented medium. These results could be used to improve the cost‐efficiency of the fermentation.

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The Biotechnology of Quorn Mycoprotein: Past, Present and Future Challenges

Whittaker

Johnson

Finnigan

et al. 2020

Grand Challenges in Biology and Biotechnology

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Mutants with general growth rate advantages are the predominant morphological mutants to be isolated from the Quorn® production plant

Cited by 10 publications

References 20 publications

Repression of deoxynivalenol accumulation and expression ofTrigenes inFusarium culmorumby fungicidesin vitro

Repression of deoxynivalenol accumulation and expression ofTrigenes inFusarium culmorumby fungicidesin vitro

Peptone changes the timing and accumulation of morphological mutants of the Quorn® myco-protein fungus Fusarium graminearum A3/5 in glucose-limited chemostat cultures

The Biotechnology of Quorn Mycoprotein: Past, Present and Future Challenges

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