Mutagenicity of urine from rats and mice treated with acrylonitrile

Urinary metabolites of [1,2,3-13C]acrylonitrile in rats and mice detected by carbon-13 nuclear magnetic resonance spectroscopy

“…Following administration of AN iv, ip, or po, greater excretion of thiocyanate was observed in mice compared with rats (9), suggesting a difference in the extent of metabolism to CEO, or its further metabolism to release cyanide. Lambotte-Vandepaer et al (39) reported a similar difference in thiocyanate excretion after ip administration of AN to rats and mice, but observed little difference between species for the excretion of Nacetyl-S-(2-hydroxyethyl)cysteine or IV-acetyl-S-(2cyanoethyl)cysteine. The results of our studies and other investigations2 indicate that the major quantitative differences between rat and mouse become fully apparent only on measurement of additional metabolites such as thiodiglycolic acid, S-(carboxymethyl)cysteine or its Nacetyl derivative, thionyldiacetic acid, and N-acetyl-S-(lcyano-2-hydroxyethyl)cysteine.…”

Carcinogenicity and Other Health Effects of Acrylonitrile with Reference to Occupational Exposure Limit.

“…In many of these experiments, metabolic activation with liver microsome fraction was required for induction of mutagenic response, but not essential. Urine from rats and mice receiving intraperitoneal injection of AN was positive to Ames test in the absence of S-9 mix (Lambotte-Vandepaer et al, 1980, 1981) 51,52) . The same was true on rats orally treated with AN (Lambotte-Vandepaer et al, 1985) 53) .…”

Mutagenicity, Carcinogenicity, and Teratogenicity of Industrial Pollutants

“…Phenobarbital pretreatment of rats did not significantly influence the excretion of thiocyanate in urine (Gut et al, 1975;Nerudova et al, 1978), and did not stimulate acrylonitrile metabolism in perfused rat liver (Nerudova et al, 1978). The same pretreatment did not intensify the S9 mediated mutagenic response of acrylonitrile and suppressed the mutagenic activity detected in urine (de Meester et al, 1979a, Lambotte-Vandepaer et al, 1980. Pretreatment with COCb or addition of SKF 525-A or CO to the incubation mixture inhibited acrylonitrile metabolism to cyanide.…”