Mutagenesis at the ad-3A and ad-3B loci in haploid UV-sensitive strains of Neurospora crassaI. Development of isogenic strains and spontaneous mutability

Serres, Frederick J. de; Inoue, Hirokazu; Schüpbach, M.E.

doi:10.1016/0027-5107(80)90006-8

Cited by 39 publications

(8 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Properties of the uvs mutants are summarized in references 509, 537, Slightly increased sensitivity to UV (187,273). Spontaneous and UV-induced mutation probably normal (273,275). Homozygous fertile and without effect on crossing over.…”

Section: (S04)mentioning

confidence: 99%

“…No dimer excision (1164). Normal spontaneous mutation (275). High UV-induced mutation rate (273); for mutation induction by other agents, see references 509 and 940. Homozygous fertile; no effect on meiosis or crossing over.…”

Section: (S04)mentioning

confidence: 99%

“…For mutation induction by other agents, see references 509 and 940. Increased spontaneous mutation (275,537). Dimer excision delayed and at reduced rate (1164).…”

Section: (S04)mentioning

confidence: 99%

“…Causes increased duplication instability (mitotic recombination or deletion or both) (932). Conidial viability is low (275,932). Double mutant upr-J; uvs-3 is much more sensitive to UV than is either single mutant (1095).…”

Section: (S04)mentioning

confidence: 99%

See 3 more Smart Citations

Chromosomal loci of Neurospora crassa

Perkins¹,

Radford²,

Newmeyer³

et al. 1982

Microbiol Rev

259

View full text Add to dashboard Cite

I I ' ~~~~T h Ñ CiOr(r r N z ADDITIONAL LOCI IN LINKAGE GROUP ILinked to mt (8 to 12%) Linked to mt, acr-3 (5%) Right of arg-i (5/29) Linked to so and aro-8 Right of un-18 Between ad-5 and the centromere Between arg-i (4%) and his-3 (I to 2%) Between In(H4250) and T(39311) Left of mt (23%) Linked to mt (2%) and aza-i (39%o) Left of nit-2 (30%) Left of mt (14%) Linked to un-i (9%), probably to the right Linked to hom (1%), probably to the right Linked to mt (17 to 22%) Between T(NM103) and al-2 (18%) Between T(4540) and cr-2Between thi-i (12 to 20%/6) and ad-9 (5%) Linked to ad-5 (1/54), probably to the left Linked to mt (6%) Linked to cys-5 (<1%), probably to the right Right of ad-9 (12%), linked to al (0/76) Right of his-3 (2%) Between the T(AR173) breakpoints Linked to al-2 (10%) and nic-i (1 to 5%) Linked to ad-9 (0/44); right of thi-i (2%) Linked to mt (5%), probably to the left Linked to al-I (10%) Right of os-i (3%) Between nit,i (5 to 19%) and al-I (6 to 19%io) Linked near arg-i Left of al-2 (25%o) Left of mt (16%) Right of nic-2 (6%) Linked to ad-9 (2%), probably to the right Left of cr-i (15%) Left of mt (10%) Probably between his-2 (3%) and cr-i (3%) Between T(NM103) and the right tip Between mt (20%) and arg-i (1%) Between ad-3 (6%) and al-i (16%) Between his-3 and nic-2 Between cyh-i (5%) and al-I (7%) Linked to cyh-i (18%), al-i (2%), and mb-2 (6%) Between arg-i (3%) and T(39311) Between tre (41%) and ad-9 Linked to mt (9%) Linked to mt (20%) Complex phenotype. Alternative requirements: 2-acetylaminofluorine, certain azo dyes, or certain single amino acids. Cold sensitive. Originated among progeny of a rib-i strain that had become tolerant to 2-acetylaminofluorine.

show abstract

Section: (S04)mentioning

confidence: 99%

Section: (S04)mentioning

confidence: 99%

“…For mutation induction by other agents, see references 509 and 940. Increased spontaneous mutation (275,537). Dimer excision delayed and at reduced rate (1164).…”

Section: (S04)mentioning

confidence: 99%

Section: (S04)mentioning

confidence: 99%

See 2 more Smart Citations

Chromosomal loci of Neurospora crassa

Perkins¹,

Radford²,

Newmeyer³

et al. 1982

Microbiol Rev

259

View full text Add to dashboard Cite

show abstract

“…Three mutants, uvs-3, mus-9 and mus-11, which show high sensitivity to ionizing radiation, MMS and histidine, belong to the uvs-3 group. They are all sterile or barren in homozygous crosses, and show high levels of spontaneous mutation and low or no mutagen-induced mutation (de Serres et al 1980;KS Ï fer 1981;KsÏ fer and Perlmutter 1980), suggesting that they may also be involved in recombination repair.…”

Section: Introductionmentioning

confidence: 96%

A Neurospora Double-Strand-Break repair gene, mus-11, encodes a RAD52 homologue and is inducible by mutagens

et al. 2000

View full text Add to dashboard Cite

We isolated a Neurospora crassa cDNA that encodes a Rad52 homologue (ncRAD52) by PCR, using degenerate primers. RFLP mapping demonstrated that the cloned gene is located close to the ro-4 locus on the right arm of linkage group V (LGVR). In a second experiment, we used sib selection to identify a cosmid clone containing the mus-11 gene in a N. crassa genomic library. Fine-scale mapping of the mus-11 mutant showed the gene order on LGVR near ro-4 to be: ad-7 - (9.5 mu) - pab-2 (7.8 mu) - mus-11 - (3.7 mu) - inv. The nucleotide sequence of the mus-11 gene matched that of the ncRAD52 cDNA. Thus, the mus-11 gene encodes the Rad52 homologue. The deduced amino acid sequence of the MUS11 protein shows 32.0% and 27.5% overall identity to the Schizosaccharomyces pombe Rad22 protein and the human hRad52 protein, respectively, and a higher level of identity (55-66%) within the conserved N-terminal region (141 residues). The MUS11 protein shows homology to Rad52 from budding yeast only within the N-terminal region (53.2% identity over 141 amino acids) which is conserved among Rad52 homologues. Yeast two-hybrid analysis reveals that the MUS11 protein binds to both the MEI-3 protein, a Rad51 homologue, and to itself in vivo. An ncRAD52 mutant obtained by the RIPping procedure showed the same sensitivity as the original mus-11 mutant to the following mutagens and chemicals: UV light, 4NQO (4-nitroquinoline 1-oxide), MMS (methyl methanesulfonate), EMS (ethyl methanesulfonate), MNNG (N-methyl-N'-nitro-N-nitrosoguanidine), TBHP (tert-butyl hydroperoxide), HU (hydroxyurea) and histidine. Unlike the RAD52 transcript in Saccharomyces cerevisiae, the mus-11 transcript could not be detected in mycelium under normal growth conditions, but expression of the gene was induced by UV irradiation or treatment with MMS.

show abstract