Muscle satellite cells and regulation of recovery potential of muscle

Ozernyuk, N. D.; Balan, O. V.

doi:10.1134/s1062359007060027

Cited by 5 publications

(1 citation statement)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mef2C was also upregulated by day 1 in both cell populations, reaching statistical significance by day 2, but unlike MyoD, the expression of this gene remained elevated throughout the 6-d culture period. Myogenin (MYOG), Troponin T (TNNT), and MYH2 represent late markers of muscle cell differentiation (Burattini et al 2004 ; Ozernyuk and Balan 2007 ). Similar expression trends with these markers were seen in HSMM and SkMDC cultures.…”

Section: Resultsmentioning

confidence: 99%

Characterization of primary human skeletal muscle cells from multiple commercial sources

Owens

Moreira

Bain

2013

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

There is a significant unmet need for safe, anabolic muscle therapies to treat diseases and conditions associated with severe muscle weakness and frailty. The identification of such therapies requires appropriate cell-based screening assays to select compounds for further development using animal models. Primary human skeletal muscle cells have recently become available from a number of commercial vendors. Such cells may be valuable for studying the mechanisms that direct muscle differentiation, and for identifying and characterizing novel therapeutic approaches for the treatment of age- and injury-induced muscle disorders. However, only limited characterization of these cells has been reported to date. Therefore, we have examined four primary human muscle cell preparations from three different vendors for their capacity to differentiate into multinucleated myotubes. Two of the preparations demonstrated robust myotube formation and expressed characteristic markers of muscle differentiation. Furthermore, these myotubes could be induced to undergo morphological atrophy- and hypertrophy-like responses, and atrophy could be blocked with an inhibitor of myostatin signaling, a pathway that is known to negatively regulate muscle mass. Finally, the myotubes were efficiently infected with recombinant adenovirus, providing a tool for genetic modification. Taken together, our results indicate that primary human muscle cells can be a useful system for studying muscle differentiation, and may also provide tools for studying new therapeutic molecules for the treatment of muscle disease.

show abstract

Section: Resultsmentioning

confidence: 99%