Muricauda lutaonensis sp. nov., a moderate thermophile isolated from a coastal hot spring

Abstract

A yellow-pigmented, Gram-staining-negative, aerobic, non-motile, moderately thermophilic, rodshaped bacterium, designated strain CC-HSB-11 T , was isolated from a coastal hot spring of Green Island (Lutao), located off Taituang, Taiwan. 16S rRNA gene sequence analysis demonstrated that it shared ,94.4 % sequence similarity with Muricauda species. Menaquinone with six isoprene units (MK-6) was the major respiratory quinone and iso-C 15 : 0 , iso-C 15 : 1 G, iso-C 15 : 0 3-OH, iso-C 16 : 0 3-OH, iso-C 17 : 0 3-OH and summed feature 3 (comprising iso-C 15 : 0 2-OH and/or C 16 : 1 v7c/t) were the predominant fatty acids. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Six unidentified phospholipids and glycolipids also occurred as minor components. The DNA G+C content of strain CC-HSB-11 T was 46.4±1 mol%. On the basis of 16S rRNA gene sequence similarities with other Muricauda species and differentiating fatty acid compositions and other phenotypic data, strain CC-HSB-11 T represents a novel species in the genus Muricauda, for which the name Muricauda lutaonensis sp. nov. is proposed. The type strain is CC-HSB-11 T (5BCRC 17850 T 5KCTC 22339 T ).

Muricauda antarctica sp. nov., a marine member of the Flavobacteriaceae isolated from Antarctic seawater

“…The DNA G+C content of strain T was 44.8 mol%, a value in the range reported for members of the genus Muricauda, i.e. 41-51 mol% (Arun et al, 2009;Hwang et al, 2009). These chemotaxonomic data support the result of the phylogenetic analysis, suggesting that strain T belongs to the genus Muricauda.…”

“…The requirement for artificial sea-salt was evaluated in trypticase/soy broth (Difco) with the addition of 0, 0.5, 1, 2, 3, 5, 7, 9, 10, 11, 12 and 15 % (w/v) artificial sea-salt (Sigma) (Yoon et al, 2005;Arun et al, 2009).The pH range for growth was determined in MB that was adjusted to pH 5-10.5 (in 0.5 pH unit intervals) using appropriate biological buffers (MES for pH 5.0-6.0, PIPES for pH 6.5-7.0, Tricine for pH 7.5-8.5 and CAPSO for pH 9.0-10.5) at a concentration of 50 mM. The temperature range for growth was determined by incubation at 4,10,15,20,25,30,35,37,40,45 and 50 u C. Cell motility and morphology were examined using optical microscopy (BX40, Olympus) and electron microscopy (S260, Cambridge; JEM-1230, JEOL).…”

Genome-Based Taxonomic Classification of Bacteroidetes

“…Support was weak (<60%), however, and in the UCT and CCT Croceitalea and Muricauda appear as separate groups with moderate support. Thus, even though in the description of the moderate thermophile M. lutaonens the genus Croceitalea was not considered (Arun et al, 2009), our analyses do not indicate a need to revise the classification of the two genera.…”

“…The description is as given by Arun et al (2009) with the following restriction. The genomic G+C content of the type strain is 45.0%.…”

Spongitalea numazuensis gen. nov., sp. nov., a new marine bacterium of the family Flavobacteriaceae isolated from an unidentified marine sponge

“…1) (Ivanova et al, 2001;Nedashkovskaya et al, 2003Nedashkovskaya et al, , 2004Nedashkovskaya et al, , 2006Nedashkovskaya et al, , 2007Sun et al, 2010); 8, Muricauda sp. (Bruns et al, 2001;Yoon et al, 2005Yoon et al, , 2008Arun et al, 2009;Hwang et al, 2009;Lee et al, 2012); 9, Robiginitalea sp. (Cho and Giovannoni, 2004;Manh et al, 2008); 10, Vitellibacter sp.…”