Multivariate Optimization and Supplementation Strategies for the Simultaneous Production of Amylases, Cellulases, Xylanases, and Proteases by Aspergillus awamori Under Solid-State Fermentation Conditions

Abstract

The production of extracts containing a pool of enzymes for extensive biomass deconstruction can lead to significant advantages in biorefinery applications. In this work, a strain of Aspergillus awamori IOC-3914 was used for the simultaneous production of five groups of hydrolases by solid-state fermentation of babassu cake. Sequential experimental design strategies and multivariate optimization using the desirability function were first used to study temperature, moisture content, and granulometry. After that, further improvements in product yields were achieved by supplementation with other agro-industrial materials. At the end of the study, the production of enzymes was up to 3.3-fold increased, and brewer's spent grains and babassu flour showed to be the best supplements. Maximum activities for endoamylases, exoamylases, cellulases (CMCases), xylanases, and proteases achieved were 197, 106, 20, 835, and 57 U g(-1), respectively. The strain was also able to produce β-glucosidases and debranching amylases (up to 35 and 43 U g(-1), respectively), indicating the potential of its enzyme pool for cellulose and starch degradation.

Aspergillus awamori positively impacts the growth performance, nutrient digestibility, antioxidative activity and immune responses of growing rabbits

“…The possible reason for the enhanced digestibility coefficients is associated with the potential role of A. awamori to facilitate the digestion of fibres and protein through the secretion of cellulase, xylanase and protease (Saleh, Hayashi, Ijiri, & Ohtsuka, 2014). Furthermore, Aspergillus can degrade the antitrypsin and antinutritional factors which are presented in soybean meal and the other plant originated ingredients and in turn increase the protein and lipids absorption in the gut (de Castro, Castilho, & Freire, 2015; Yamamoto, Saleh, Ohtsuka, & Hayashi, 2005). In parallel with the current study, Saleh, Amber, et al, 2014 reported that broilers fed diets with A. awamori displayed increased protein, lipid and fibre digestibility coefficients.…”

Potential application of waste from castor bean (Ricinus communis L.) for production for xylanase of interest in the industry

“…In current research, A. japonicus URM5620 was a higher xylanase producer (29,085 ± 1808 U/g ds; Table 1 ) compared to A. fumigatus SK1 under SSF using untreated oil palm trunk (418.7 U g −1 ) and by Aspergillus awamori IOC-391 using babassu cake (835.0 U g −1 ; Castro et al 2015 ).…”

Fermentação De Farelo De Mandioca, Para a Obtenção De Xilanase, a Partir De Um Fungo Endofítico

“…Várias pesquisas com o intuito de estudar moléculas, como as xilanases microbianas, com alto valor agregado, têm despertado reconhecido potencial biotecnológico e está inserido em um mercado em expansão (CASTRO et al, 2015;PÉREZ-RODRÍGUEZ et al, 2015). Apesar da relevância, a produção de valores significativos de proteína extracelular está associada a vários fatores (temperatura, umidade e tempo de cultivo) e ainda é restrita a alguns microrganismos.…”