Multiplicação in vitro e aclimatação de Vernonia condensata Baker

Vicente, Meza; Almeida, Weliton Antônio Bastos de; Carvalho, Zuleide Silva de

doi:10.1590/s1516-05722009000200011

Cited by 22 publications

(21 citation statements)

References 11 publications

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“…According to Shukla and Sawhney (1991), the presence of both BAP and ANA stimulates shoot production in several types of Brassica explants, but the response depends on the concentration used. Vicente et al (2009) also reported that high BAP concentrations reduced the NSE, most likely due to the phytotoxic effect of the cytokinin, which is consistent with the observed reduction in NSE at 120 days in the presence of 5 µM BAP.…”

Section: Resultssupporting

confidence: 82%

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Werner¹,

Soares²,

Gontijo³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar 'FMS brilhante' using polymerase chain reaction techniques based on inter-simple sequence repeat (ISSR) molecular markers. The aim was to develop a protocol for the in vitro regeneration of these plants with low genetic variation as compared to the donor plant. For micropropagation, shoot tips from in vitro germinated seedlings were used as explants and were initially cultivated for 90 days on MS medium with 5.0 µM 6-benzylaminopurine (BAP), which at 90 days, led to the highest number of shoots per explant (NSE) (12.20 shoots) being detected. After 120 days, the interaction between BAP concentration and naphthalene acetic acid (NAA) was tested, and the highest NSE was observed following exposure to 0.0/0.5 µM BAP/NAA (11.40 shoots) and 1.0/0.0 µM BAP/NAA (11.00 shoots). The highest proportion of rooting phase were observed following exposure to 0.5 µM NAA (30%). The 13 ISSR primers used to analyze genetic stability produced 91 amplification products, of which only eight bands were polymorphic and 83 were monomorphic for all 10 regenerated crambe plants, compared to the donor plant explant. These results indicate that crambe shoot tips are a highly reliable explant that can be used to micropropagate genetically true-to-type plants or to maintain genetic stability, as verified using ISSR markers.

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Section: Resultssupporting

confidence: 82%

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Werner¹,

Soares²,

Gontijo³

et al. 2015

Genet. Mol. Res.

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“…The surviving explants (shoots of nodal segments) were placed in 268-mL flasks containing 20 mL of MS culture medium supplemented with 1.0 mg L -1 of BAP (6-benzylaminopurine), according to VICENTE et al (2009), under the previously described growth room conditions, to increase the number of shoots over another 30 days.…”

Section: In Vitro Multiplicationmentioning

confidence: 99%

“…Almeida et al The National List of Medicinal Plants of Interest to the Public Health System (Relação Nacional de Plantas Medicinais de Interesse ao Sistema Único de Saúde -RENISUS) currently contains 71 species, including Vernonia condensata Baker (BRASIL, 2010). This plant is commonly known in Brazil as alumã (VICENTE et al, 2009) and boldo-da-bahia (AFONSO et al, 2015), among other names. It belongs to the family Asteraceae and has been used since colonial times to treat gastrointestinal problems and because of its analgesic properties (BOORHEM, 1999).…”

Section: Introductionmentioning

confidence: 99%

Vernonia condensata Baker: an alternative for large-scale seedling production

Almeida¹,

Oliveira²,

Jacobi³

et al. 2020

Cienc. Rural

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The increasing use of Vernonia condensata Baker highlights the importance of developing strategies to reduce the impact of exploitation on nature reserves. The aim of this study was to establish a micropropagation protocol to produce homogenous plants with high phytosanitary quality. Apical, nodal, and internodal segments of plants grown in the field were used for in vitro growth. The segments were disinfected in sodium hypochlorite solution (1.0 and 2.0%) for 15 and 30 minutes and then transferred to Petri dishes containing MS culture medium for 30 days. A completely randomized factorial experiment (3 x 2 x 2) with five replicates was designed. After this period, a completely randomized in vitro multiplication experiment was carried out with six treatments (BAP - 0.0; 0.5; 1.0; 1.5; 2.0; 2.5 mg L-1) and six replicates. The shoots obtained in the best treatment were transferred to flasks with rooting medium (MS, MS/2 or MS/4). The experiment was completely randomized with 12 replicates. Microplants were acclimatized in polyethylene terephthalate (PET) bottles filled with autoclaved topsoil. Our results showed that 40.0% of the nodal segments (immersed in 1.0% sodium hypochlorite for 30 minutes) were adequately disinfected and survived. In the in vitro multiplication experiment, the 0.5 mg L-1 concentration of BAP yielded the highest number of shoots and the best vegetative growth. With regard to the assessed characteristics, MS/4 was the best rooting medium, with 100% survival during acclimatization. This study showed that V. condensata in vitro culture might produce 32,000 seedlings in 7 months.

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“…No entanto, este efeito nem sempre é conseguido, pois vários fatores podem interferir, tais como espécie em questão, o tipo de explante, dentre outros (ROGALSKI et al, 2003;DUTRA et al, 2004;MENDONÇA et al, 2004;BERTONI et al, 2006;RUBIN et al, 2007;RODRIGUES et al, 2009;VICENTE et al, 2009 MS 83,33a 95,83a 1,99a 2,96a 1,00a 1,17a 100,00a 100,00a 2,92b 3,33b 37,50a 29,17a 1,30a 1,74a BAP 65,27a 76,39b 1,57a 1,94a 1,03a 1,24a 98,61a 98,61a 4,51a 4,35a 16,67a 34,72a 1,09b 1,38b Cinetina 59,72a 72,22b 1,36a 2,18a 1,01a 1,09a 97,22a 100,00a 3,36b 3,47b 33,33a 22,22a 1,03b 1,35b Zeatina 72,22a 75,00b 1,80a 2,53a 1,08a 1,21a 91,94b 91,67a 2,32b 2,72b 38,89a 45,83a 1,34a 1,78a…”

Section: Resultsunclassified

“…No entanto, muitos experimentos conseguem com sucesso a aclimatação de plantas micropropagadas (GIRARDI et al, 2007;PORTELA et al, 2009;VICENTE et al, 2009). …”

Section: Etapas Básicas Da Micropropagaçãounclassified

Estudo agronômico, químico e biológico de cochlospermum regium (mart. ex. scharank): uma planta medicinal do Cerrado

Inácio¹

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III "Apesar dos nossos defeitos, precisamos enxergar que somos pérolas únicas no teatro da vida e entender que não existem pessoas de sucesso e pessoas fracassadas. O que existem são pessoas que lutam pelos seus sonhos ou desistem deles." Augusto Cury IV AGRADECIMENTOSA Deus, o sábio dos sábios, por sempre me mostrar o melhor caminho a seguir, por ser o alicerce da minha vida e por nunca me abandonar.Ao Tiago, meu parceiro, aquele que permaneceu comigo em todos os momentos difíceis desta caminhada e por compartilharmos juntos nossos sonhos.Ao meu pai, João, pelo amor incondicional e aceitação das minhas escolhas.Aos meus irmãos, Johnny e Débora, por fazerem parte da minha vida.A todos os familiares, inclusive os adquiridos durante a minha vida, os de coração, e amigos que acreditaram em mim, que vibraram e sentiram-se orgulhosos com minhas conquistas.A minha querida orientadora e mãe de coração, Ana Maria Soares Pereira, que me dá suporte não só profissional, mas um carinho especial que me conforta sempre.A profª Drª Bianca Waléria Bertoni, pela atenção nunca negada e sempre oferecida com sorriso, pelo suporte neste aprendizado, e pela sua valiosa contribuição como membro da Banca Examinadora.A querida profª Drª Giuseppina Pace Pereira Lima por fazer parte da Banca Examinadora e contribuir tão grandiosamente para minha formação e aperfeiçoamento.Aos meus amigos de laboratório: pelo auxílio e pelas boas horas de alegria. A Universidade de Ribeirão Preto (UNAERP), em especial a Profª Suselei de CastroFrança, por me receberem de portas abertas para desenvolver meu projeto de mestrado.A todos os funcionários e professores da UNAERP pelo auxílio e por me receberem tão calorosamente.A UNESP, por me darem a chance de crescer profissionalmente.A FAPESP, pela bolsa concedida e pelo financiamento do projeto. ....................................................................................................

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Multiplicação in vitro e aclimatação de Vernonia condensata Baker

Cited by 22 publications

References 11 publications

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Vernonia condensata Baker: an alternative for large-scale seedling production

Estudo agronômico, químico e biológico de cochlospermum regium (mart. ex. scharank): uma planta medicinal do Cerrado

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