Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Werner, Elias Terra; Soares, Taís Cristina Bastos; Gontijo, Andréia Barcelos Passos Lima; Neto, J. D. Souza; Amaral, José Augusto Teixeira do

doi:10.4238/2015.december.9.16

Cited by 9 publications

(5 citation statements)

References 32 publications

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“…For the commercial utilization of tissue culture technique, it is necessary to evaluate the genetic fidelity of in vitro regenerants. Among molecular markers, ISSR markers being polymorphic, reproducible and informative is widely used for estimating genetic stability of tissue culture-derived plants (Xing et al 2010;Werner et al 2015;Saha et al 2016;Tripathi et al 2018). Molecular markers along with flow cytometry technique have been successfully employed for the assessment of genetic uniformity of in vitro regenerants (Liu et al 2011;Alatar et al 2017;Konar et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Rapid plant regeneration in industrially important Curcuma zedoaria revealing genetic and biochemical fidelity of the regenerants

et al. 2019

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The present investigation was carried out to establish an efficient and reproducible micropropagation protocol for the production of morphologically, genetically and chemically uniform plants of Curcuma zedoaria. Axillary bud explants of C. zedoaria were inoculated into MS basal medium supplemented with various combinations and concentrations of 6-benzyladenine (2.2-22.2 µM, BA), kinetin (2.3-23.2 µM, Kin), indole-3-acetic acid (2.9-11.4 µM, IAA), α-naphthalene acetic acid (2.7-10.2 µM, NAA) and adenine sulphate (33.9-203.6 µM, Ads). Almost 95% of rhizome buds sprouted on MS medium supplemented with 13.3 μM BA, 5.7 μM IAA and 63.9 μM Ads giving rise to an average of 12.89 ± 0.02 shoots within 6 weeks. However, the maximum number of roots (25.8 ± 0.07 roots per explant) was obtained on half strength MS medium supplemented with 7.4 µM of IBA after 4 weeks of inoculation. Morphological characteristics were similar in both conventionally propagated and micropropagated plants. Additionally, genetic homogeneity of in vitro plants was further confirmed through ISSR and flow cytometry analysis. A total of 27 ISSR primers were screened, out of which 13 ISSR primers generated 58 monomorphic and reproducible bands thereby confirming the genetic uniformity of obtained plants. The mean 2C DNA content of the mother plant (2.96 pg) was similar to that of in vitro derived plants (3.07 pg). Gas chromatographymass spectrometry (GC-MS) analysis showed similarity in the qualitative profile of chemical constituents of essential oil and high-performance liquid chromatography analysis revealed no significant differences in curcumin content in the tissue culture regenerants and mother plants of C. zedoaria. Therefore, the present micropropagation protocol could be effectively employed to generate true to type plantlets of C. zedoaria.

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Section: Introductionmentioning

confidence: 99%

Rapid plant regeneration in industrially important Curcuma zedoaria revealing genetic and biochemical fidelity of the regenerants

et al. 2019

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“…1). Cultivation on the medium with kinetin in combination with 0.1-0.5 mg/L NAA (№13, 14,18,19,21,22) showed the regeneration activity of leaves up to 40 %. Further increase in the NAA content suppressed the plantlets formation.…”

Section: Resultsmentioning

confidence: 99%

“…Total DNA was isolated with the modified CTAB method [18]. The primer sequences for SSR loci gHGL218091, gHGL110933, gH032602 [19], Na10-F08 [20] and ISSR primers UBC827, UBC864, UBC890 [21], A17898, B17899, IS-05 [22] were used in the study.…”

Section: Methodsmentioning

confidence: 99%

Crambe aspera plants in vitro propagation and its effect on fatty acids and phenolic compounds content and genome stability

et al. 2019

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In vitro culture can be used for endangered species preservation but its effect on biochemical properties and genetic stability of plants requires further research. Aim. The study on fatty acids content, phenolic compounds and phylogenetic relationships of Crambe aspera plants upon aseptic cultivation in vitro. Methods. Morphogenic potential of Crambe aspera leaf, root and petiole explants was studied in the Murashige and Skoog (MS) medium with different concentrations of growth factors. Fatty acids (FA) content was determined by gas chromatography-mass spectrophotometry of FA ethers, phenolic compounds were determined by spectrophotometry. Polymerase chain reactions were used to study phylogenetic relationships. Results. The efficient protocols of seed surface sterilization as well as methods of fast microclonal multiplication and obtention of C. aspera callus tissue for were developed. Conclusions. Leaves of both in vitro and in vivo cultured plants had a high content of α-linolenic acid whereas erucic acid was absent. At the same time, the difference in biochemical composition between the plants grown in aseptic and non-aseptic conditions was shown. In vivo populations of C. aspera showed a high level of polymorphism but its genome did not undergo changes after the in vitro establishment.

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“…These variations could be due to factors such as explant type, culture medium composition, culture duration, phytoregulators, genotype, and number of subcultures or transfers. All these conditions are considered capable of inducing variability on in vitro cultures [40][41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Vital Parameters Assessments of Starvation Tolerance of in vitro Populus alba Culture

Ahmed¹,

Ahmed²,

Aloufi³

et al. 2022

Phyton

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Populus alba is a large woody deciduous plant. The plant has been introduced to shooting, then multiplication of rooting on Murashige and Skoog (MS) medium. This work was designed to estimate the effect of two factors (low levels of 1-Naphthaleneacetic acid NAA and sucrose) on P. alba response resulting in 6 treatments compared to the control, with twelve measured responses. There was a significant difference in some measurements in morphology, like plantlets fresh-weight, shoot-, root-length, and leaf number. In the physiological measurements, there were significant differences in all the measured parameters. The low concentrations of sucrose and media composition/power (MS grams/L) led to starvation in plants; however, these conditions led to enhancement in some morphological and physiological parameters to overcome the starvation effect, compared to the control. The RAPD-PCR molecular marker (four decamers) was used to evaluate the new individuals' genetic variation (instability), resulting in a total polymorphism percentage of 50.83%. It was formerly known that the plantlets were identical to each other and to the mother plant. In this study, however, the use of distinct media power, hormonal and sucrose levels resulted in molecular variation reflected in P. alba's morphological and physiological responses.

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Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Cited by 9 publications

References 32 publications

Rapid plant regeneration in industrially important Curcuma zedoaria revealing genetic and biochemical fidelity of the regenerants

Rapid plant regeneration in industrially important Curcuma zedoaria revealing genetic and biochemical fidelity of the regenerants

Crambe aspera plants in vitro propagation and its effect on fatty acids and phenolic compounds content and genome stability

Vital Parameters Assessments of Starvation Tolerance of in vitro Populus alba Culture

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