Multiple bovine papillomavirus infections associated with cutaneous papillomatosis in brazilian cattle herds

Claus, Marlise Pompeo; Lunardi, Michele; Alfieri, Amauri Alcindo; Otonel, Rodrigo Alejandro Arellano; Sartori, Daniele; Fungaro, Maria Helena Pelegrinelli

doi:10.1590/s1516-89132009000700013

Cited by 25 publications

(19 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In conclusion, most reports of BPV-1 cases describe the virological aspects, despite the presence of pathomorphological and immunohistochemical studies (2,4,6,8,11,23). However, some studies have demonstrated relationships between other E genes and apoptosis (16).…”

Section: Tab 4 Percentage (%) Of All Reactions Belonging To Immunohmentioning

confidence: 91%

Investigation of E2, E5, and E6 gene expression and DNA in situ fragmentation findings associated with progressive and regressive changes in benign neoplastic cutaneous lesions arising naturally from bovine papillomavirus-1 infection

Alçığır¹,

Vural²,

Timurkan³

2016

Medycyna Weterynaryjna

View full text Add to dashboard Cite

This study investigated the progressive and regressive phases of bovine papillomavirus (BPV)-1-induced papilloma and fibropapilloma in terms of the distribution of viral antigenicity; E2, E5, and E6 gene activity and expression; and apoptosis (evaluated using DNA in situ fragmentation). All samples from 74 bovine cases of cutaneous lesions with suspected neoplastic changes collected from different skin areas were evaluated. The lesions were diagnosed as papilloma or fibropapilloma using routine hematoxylin–eosin and Mallory’s trichrome staining protocols. Feulgen reactions suggested a possible viral entity, and BPV-1 antigenicity was immunohistochemically observed in 22 cases. E gene expression in neoplastic tissues was evaluated using chromogenic in situ hybridization and reverse-transcription polymerase chain reaction, and the TUNEL method was used to evaluate DNA in situ fragmentation. Differences between progressive and regressive phases in these lesions were analyzed. Ultimately, the number of cases in the progressive phase (18) and regressive phase (4) were determined. Although E2 and E5 gene expression was observed in both phases, E6 gene distribution differed from the other E genes distribution in the progressive phase. E5 gene expression appeared stronger in oncogenic fibrocytes and fibroblasts, whereas E2 and E6 gene expression was weaker in epidermal cells and cells in the regressive phase. Apoptosis, considered the best indicator of neoplastic regression, was found to be associated with the regressive phase but was observed insufficiently in epidermal and dermal samples of the progressive-phase cases. In conclusion, this work provides significant insights on the molecular and immunopathological features of BPV-1.

show abstract

Section: Tab 4 Percentage (%) Of All Reactions Belonging To Immunohmentioning

confidence: 91%

Investigation of E2, E5, and E6 gene expression and DNA in situ fragmentation findings associated with progressive and regressive changes in benign neoplastic cutaneous lesions arising naturally from bovine papillomavirus-1 infection

Alçığır¹,

Vural²,

Timurkan³

2016

Medycyna Weterynaryjna

View full text Add to dashboard Cite

show abstract

“…Moreover, the results obtained through diagnostic techniques allow drawing a molecular epidemiology of the infections, which has prophylactic importance, since immunity is species-specific (Claus et al, 2009). The limited number of BPV types described compared to human papillomavirus (HPV) reflects a small sample size analyzed and not a lack of antigenic diversity for BPVs (Claus et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Reports available in different regions of the country indicate a significant diversity of viral types, implying evident disease burden (Yaguiu et al, 2008;Claus et al, 2009;Carvalho et al, 2012;Lunardi et al, 2013). Thus, it is critical to identify the BPV types and their prevalence, distribution and clinical consequences for the development of prophylactic and/or therapeutic procedures.…”

Section: Introductionmentioning

confidence: 99%

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

et al. 2014

View full text Add to dashboard Cite

ABSTRACT. Bovine papillomavirus (BPV) is an oncogenic virus associated with benign and malignant lesions, which result in notable economic losses. Peripheral blood samples and cutaneous papillomas were obtained from four adult beef cattle. Viral molecular identification was performed using specific primers for BPV-1, -2 and -4 in blood diagnosis and FAP59/FAP64 for skin papillomas. Histopathologic examination was done as a complementary and differential diagnosis. The fragments were purified, sequenced, and compared using BLASTn. The blood diagnosis showed the presence of BPV-2 and the analysis of cutaneous papillomas showed the presence of BPV-4, a new First description of BPV-12 and putative type BAPV8 in Brazil putative virus type BAPV8, and BPV-12, revealing for the first time the presence of BPV-12 and the putative type BAPV8 in beef cattle in Brazil. The sequences were deposited in the GenBank. Histopathology revealed acanthosis, hyperkeratosis, and koilocytosis in all samples analyzed. The presence of BAPV8 and BPV-12 in Brazil emphasizes the ubiquitous dissemination of BPVs in the herds of Brazil.

show abstract

“…Unfortunately, BPV epidemiological surveys are still limited by the availability of high-throughput diagnostic techniques that could discriminate different BPV sequences at the same time in co infected samples [16, 26]. In this context, the present work represents an effort to identify BPV types employing an alternative screening method based on the PCR-RFLP technique and correlating the histological data of the analyzed lesions with the diagnosed viral type.…”

Section: Introductionmentioning

confidence: 99%

Bovine Papillomavirus in Brazil: Detection of Coinfection of Unusual Types by a PCR-RFLP Method

Carvalho

Sakata

Giovanni

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

Bovine papillomavirus (BPV) is recognized as a causal agent of benign and malignant tumors in cattle. Thirteen types of BPV are currently characterized and classified into three distinct genera, associated with different pathological outcomes. The described BPV types as well as other putative ones have been demonstrated by molecular biology methods, mainly by the employment of degenerated PCR primers. Specifically, divergences in the nucleotide sequence of the L1 gene are useful for the identification and classification of new papillomavirus types. On the present work, a method based on the PCR-RFLP technique and DNA sequencing was evaluated as a screening tool, allowing for the detection of two relatively rare types of BPV in lesions samples from a six-year-old Holstein dairy cow, chronically affected with cutaneous papillomatosis. These findings point to the dissemination of BPVs with unclear pathogenic potential, since two relatively rare, new described BPV types, which were first characterized in Japan, were also detected in Brazil.

show abstract

Multiple bovine papillomavirus infections associated with cutaneous papillomatosis in brazilian cattle herds

Cited by 25 publications

References 22 publications

Investigation of E2, E5, and E6 gene expression and DNA in situ fragmentation findings associated with progressive and regressive changes in benign neoplastic cutaneous lesions arising naturally from bovine papillomavirus-1 infection

Investigation of E2, E5, and E6 gene expression and DNA in situ fragmentation findings associated with progressive and regressive changes in benign neoplastic cutaneous lesions arising naturally from bovine papillomavirus-1 infection

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

Bovine Papillomavirus in Brazil: Detection of Coinfection of Unusual Types by a PCR-RFLP Method

Contact Info

Product

Resources

About