Multiple-and Cross-Resistance of Amaranthus retroflexus to Acetolactate Synthase (ALS) and Photosystem II (PSII) Inhibiting Herbicides in Preemergence

Francischini, Alessandra Constantin; Constantin, Jamil; Oliveira, Rubem Silvério de; Takano, Hudson Kagueyama; Mendes, Rafael Romero

doi:10.1590/s0100-83582019370100026

Cited by 6 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resistance level of the RRPW-R population to pyrithiobac, 1476-fold, is higher compared to resistance factors of 3 to 71 [19] and 7 to 38 [20] reported earlier for redroot pigweed from Brazil. The resistance index of 900 to trifloxysulfuron in the RRPW-R population was higher compared to redroot pigweed populations from Brazil that exhibited R/S values of 23 to 58 in 2014 [19] and 339-fold levels reported in 2019 [20]. The 2019 ALS-inhibiting herbicide resistant redroot pigweed populations from Brazil were also multiple resistant to Photosystem II (PSII) inhibitors [20].…”

Section: Discussioncontrasting

confidence: 60%

“…The resistance index of 900 to trifloxysulfuron in the RRPW-R population was higher compared to redroot pigweed populations from Brazil that exhibited R/S values of 23 to 58 in 2014 [19] and 339-fold levels reported in 2019 [20]. The 2019 ALS-inhibiting herbicide resistant redroot pigweed populations from Brazil were also multiple resistant to Photosystem II (PSII) inhibitors [20]. Resistance levels to pyrithiobac in redroot pigweed from other parts of the world have not been clearly documented.…”

Section: Discussionmentioning

confidence: 91%

“…Previously, redroot pigweed accessions/biotypes/populations resistant to one or more ALSinhibiting herbicides, including those evaluated in this research, have been reported from Brazil, Canada, China, Germany, Israel, Italy, Serbia, and the USA [5,[19][20][21]. The resistance level of the RRPW-R population to pyrithiobac, 1476-fold, is higher compared to resistance factors of 3 to 71 [19] and 7 to 38 [20] reported earlier for redroot pigweed from Brazil. The resistance index of 900 to trifloxysulfuron in the RRPW-R population was higher compared to redroot pigweed populations from Brazil that exhibited R/S values of 23 to 58 in 2014 [19] and 339-fold levels reported in 2019 [20].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Resistance to acetolactate synthase inhibitors is due to a W 574 to L amino acid substitution in the ALS gene of redroot pigweed and tall waterhemp

Nandula

Giacomini

Ray³

2020

PLoS ONE

View full text Add to dashboard Cite

Several Amaranthus spp. around the world have evolved resistance (and cross resistance) to various herbicide mechanisms of action. Populations of redroot pigweed (RRPW-R) and tall waterhemp (TW-R) in Mississippi, USA have been suspected to be resistant to one or more acetolactate synthase (ALS) inhibiting herbicides. Whole plant dose-response experiments with multiple ALS inhibitors, ALS enzyme assays with pyrithiobac, and molecular sequence analysis of ALS gene constructs were conducted to confirm and characterize the resistance profile and nature of the mechanism in the RRPW-R and TW-R populations. Two susceptible populations, RRPW-S and TW-S were included for comparison with RRPW-R and TW-R, correspondingly. The resistance index (R/S; the herbicide dose required to reduce plant growth by 50% of resistant population compared to the respective susceptible population) values of the RRPW-R population were 1476, 3500, and 900 for pyrithiobac, imazaquin, and trifloxysulfuron, respectively. The R/S values of the TW-R population for pyrithiobac, imazaquin, and trifloxysulfuron were 51, 950, and 2600, respectively. I 50 values of RRPW-S and RRPW-R populations for pyrithiobac were 0.062 and 208.33 μM, indicating that the ALS enzyme of the RRPW-R population is 3360-fold more resistant to pyrithiobac than the RRPW-S population under our experimental conditions. The ALS enzyme of the TW-R population was 1214-fold resistant to pyrithiobac compared to the TW-S population, with the I 50 values for pyrithiobac of ALS from TW-R and TW-S populations being 87.4 and 0.072 μM, correspondingly. Sequencing of the ALS gene identified a point mutation at position 574 of the ALS gene leading to substitution of tryptophan (W) residue with a leucine (L) residue in both RRPW-R and TW-R populations. Thus, the RRPW-R and TW-R populations are resistant to several ALS-inhibiting herbicides belonging to different chemical classes due to an altered target site, i.e., ALS. Resistance in Amaranthus spp. to commonly used ALS-inhibiting herbicides warrants an

show abstract

Section: Discussioncontrasting

confidence: 60%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Resistance to acetolactate synthase inhibitors is due to a W 574 to L amino acid substitution in the ALS gene of redroot pigweed and tall waterhemp

Nandula

Giacomini

Ray³

2020

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…In samples of Amaranthus retroflexus resistant to trifloxysulfuron-sodium and pyrithiobacsodium from cotton producing regions in Brazil, high resistance factors were observed both in POST and PRE [18]. With inhibitors of carotenoid synthesis, the resistance to herbicide mesotrione was confirmed in PRE in Amaranthus.…”

Section: Discussionmentioning

confidence: 91%

A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil

Silva

Mendes

Suzukawa

et al. 2022

Plants

View full text Add to dashboard Cite

Cases of weed resistant to herbicides have changed the dynamics of agricultural areas in Brazil, and in recent years, Erigeron species have caused major problems to farmers in the country, mainly in relation to the ineffectiveness of herbicide treatments used. The objective of this study was to confirm the cross-resistance to ALS inhibitors in populations of Erigeron sumatrensis as well as to investigate the existence of mutations in the site of action of ALS-inhibiting herbicides. To do this, 30 populations collected in the 2016/2017 crop season were grown in a greenhouse. Dose–response (chlorimuron-ethyl and cloransulam-methyl), inhibition of cytochrome P-450 with malathion, and ALS gene sequencing experiments were carried out in the F1 generations of two fleabane populations. The results proved the cross-resistance to chlorimuron-ethyl and cloransulam-methyl herbicides applied in the post-emergence of the resistant population of E. sumatrensis. The higher activity of P450 enzymes is unlikely responsible for the resistance of the population studied. The resistance mechanism found in R was the target site mutation Pro197Ser at the ALS gene. This is the first study in Brazil to identify a target-site change as a survival mechanism in E. sumatrensis for the resistance to ALS-inhibiting herbicides.

show abstract

“…In Brazil, there is a predominance of the species A. viridis, A. spinosus, A. deflexus, A. hybridus, A. retroflexus and A. lividus scattered all across the national territory (Francischini et al, 2019). However, in order to define, properly, the nature and/or the amount of compounds beneficial to the health in any vegetable species, it is necessary to examine the influence of the extraction process in the samples with different types of solvent and extraction methods (Azwanida, 2015) since the efficiency in the extraction of a specific compound can be related to the polarity of the solvent.…”

Section: Introductionmentioning

confidence: 99%

Nutritional and bioactive compounds of Amaranthus spp. in Brazil

Souza

Salimena²,

Xavier³

et al. 2022

RSD

View full text Add to dashboard Cite

The objective of this research was to evaluate the physicochemical characteristics of Amaranthus spp. commonly happening in Brazil, besides defining the efficiency of different extraction processes of the phenolic compounds and of the antioxidant activity. Five species of the genus Amaranthus (A. spinosus, A. viridis, A. retroflexus, A. hybridus var. paniculatus e A. deflexus) were evaluated in regard to their physicochemical characteristics such as vitamin C, soluble solids, pH, moisture, lipids and protein levels as well as the coloration of the leaves. Also, the phenolic compounds and the antioxidants were defined through four extraction processes (reflux; hydroalcoholic turbo-extraction; aqueous; and methanolic). The species A. spinosus has stood out for its vitamin C levels, pH and moisture, indicating the potential of the species when compared to the others. The leaf extracts of the five Amaranthus species present differences in the total phenol levels, flavonoids and hydro flavonoids when subject to different extraction methods. The antioxidant activities were favored by the reflux and hydroalcoholic turbo-extraction, with A. viridis standing out in the reflux methods and A. spinosus in the hydroalcoholic.

show abstract

Multiple-and Cross-Resistance of Amaranthus retroflexus to Acetolactate Synthase (ALS) and Photosystem II (PSII) Inhibiting Herbicides in Preemergence

Cited by 6 publications

References 22 publications

Resistance to acetolactate synthase inhibitors is due to a W 574 to L amino acid substitution in the ALS gene of redroot pigweed and tall waterhemp

Resistance to acetolactate synthase inhibitors is due to a W 574 to L amino acid substitution in the ALS gene of redroot pigweed and tall waterhemp

A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil

Nutritional and bioactive compounds of Amaranthus spp. in Brazil

Contact Info

Product

Resources

About