A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil

Silva, Vanessa Francieli V; Mendes, Rafael Romero; Suzukawa, Andréia Kazumi; Adegas, F. S.; Marcelino-Guimarães, Francismar Corrêa; Oliveira, Rubem Silvério de

doi:10.3390/plants11040467

Cited by 6 publications

(3 citation statements)

References 25 publications

(39 reference statements)

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“…Furthermore, a population of C. sumatrensis has already been confirmed to exhibit multiple resistance to five herbicide action sites: PSII inhibitors (paraquat), PS I Electron Diversion (diuron), protoporphyrinogen oxidase inhibition (saflufenacil), enolpyruvyl shikimate phosphate synthase inhibition (glyphosate), and auxin mimics (2,4-D) [27]. Additionally, there is a reported case of cross-resistance in C. sumatrensis to the ALS inhibitors chlorimuron-ethyl and cloransulam-methyl [28]. However, no cases of resistance to diclosulam have been reported in Conyza spp.…”

Section: Discussionmentioning

confidence: 99%

Conyza bonariensis’ Resistance to Glyphosate, Diclosulam, and Chlorimuron: Confirmation and Alternative Control for the First Case of Multiple and Cross-Resistance in Uruguay

Kaspary,

Waller Barcena,

García

et al. 2023

Agronomy

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Conyza bonariensis L. (hairy fleabane) is a significant weed in production systems, especially due to its evolving resistance to various herbicides. In Uruguay, control failures of C. bonariensis have been reported following the use of glyphosate and ALS inhibitors. The objective of this study was to investigate the occurrence of the multiple and cross-resistance of C. bonariensis to glyphosate, chlorimuron, and diclosulam, and to assess the efficacy of alternative herbicides against these resistant biotypes. Seeds were collected from sites where plants had survived herbicide applications during the 2019/2020 and 2020/2021 seasons. Following initial screenings, biotypes were selected to establish independent dose–response curves for glyphosate, diclosulam, and chlorimuron. For each herbicide, four biotypes of C. bonariensis were tested: one susceptible (S), two putatively moderately resistant (MR) biotypes, and one putatively highly resistant (R) biotype. In each assay, eight herbicide doses were used (1/32; 1/16; 1/8; 1/2; 1; 2; and 4X for S and MR biotypes, and 1/8; 1/2; 1; 2; 4; 8; and 16X for R biotypes) based on the recommended dose (1x) for each herbicide, with four repetitions per treatment. Each assay was completely replicated twice. Resistance was confirmed through testing in two plant generations (G1 and G2). The findings reveal high levels of multiple and cross-resistance in C. bonariensis to glyphosate, diclosulam, and chlorimuron. In general, herbicides with alternative action mechanisms effectively controlled C. bonariensis exhibiting multiple and cross-resistance. This study confirms the first case of C. bonariensis cross-resistance to diclosulam and chlorimuron, and the first occurrence of multiple and cross-resistance to glyphosate, diclosulam, and chlorimuron in this species.

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Section: Discussionmentioning

confidence: 99%

Conyza bonariensis’ Resistance to Glyphosate, Diclosulam, and Chlorimuron: Confirmation and Alternative Control for the First Case of Multiple and Cross-Resistance in Uruguay

Kaspary,

Waller Barcena,

García

et al. 2023

Agronomy

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“…IMIs, including imazameth, imazamox, and imazethapyr, are among the most widely utilized groups of inhibitors of ALS [ 13 ]. Moreover, mutations at specific sites within the ALS coding region have been verified as increasing crop and weed tolerance to IMIs [ 12 , 15 , 16 , 17 , 18 ]. The amino acid substitution resulting from the base mutation generates a novel ALS isoform that is less sensitive to IMIs but does not affect its enzyme activity compared with that of the wild type [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Imidazolinone Herbicide Resistance Gene with KASP Markers for Japonica/Geng Rice Varieties in the Huanghuaihai Region of China

Liu,

Feng,

Wang

et al. 2024

Plants

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Rice is a staple food for more than half of the global population due to its food security and sustainable development. Weeds compete with crops for sunlight and indispensable nutrients, affecting the yield and quality of crops. Breeding herbicide-tolerant rice varieties paired with herbicide application is expected to help with weed control. In this study, 194 Japonica/Geng rice varieties or lines collected from the Huanghuaihai region of China were screened by Kompetitive Allele-Specific PCR (KASP) markers based on four mutation sites within OsALS1 (LOC_Os02g30630), which is the target of imidazolinone (IMI) herbicides. Only the OsALS1627N haplotype was identified in 18 varieties, including the previously reported Jingeng818 (JG818), and its herbicide resistance was validated by treatment with three IMIs. To investigate the origin of the OsALS1627N haplotype in the identified varieties, six codominant PCR-based markers tightly linked with OsALS1 were developed. PCR analysis revealed that the other 17 IMI-tolerant varieties were derived from JG818. We randomly selected three IMI-tolerant varieties for comparative whole-genome resequencing with known receptor parent varieties. Sequence alignment revealed that more loci from JG818 have been introduced into IMI-tolerant varieties. However, all three IMI-tolerant varieties carried clustered third type single nucleotide polymorphism (SNP) sites from unknown parents, indicating that these varieties were not directly derived from JG818, whereas those from different intermediate improved lines were crossed with JG818. Overall, we found that only OsALS1627N from JG818 has been broadly introduced into the Huanghuaihai region of China. Additionally, the 17 identified IMI-tolerant varieties provide alternative opportunities for improving such varieties along with other good traits.

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“…Mutations in the ALS gene confer resistance to many weeds, such as Solanum ptychanthum Dunal (Solanales: Solanaceae) [ 7 ], Amaranthus palmeri S.Watson (Caryophyllales: Amaranthaceae) [ 8 ], Erigeron sumatrensis Retz. (Asterales: Asteraceae) [ 9 ], Glebionis coronaria (Linnaeus) Cassini ex Spach (Asterales: Asteraceae) [ 10 ], etc.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Resistance to Nicosulfuron in Setaria viridis

Cao

Lan

Huang

et al. 2023

IJMS

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The green foxtail, Setaria viridis (L.) P. Beauv. (Poales: Poaceae), is a troublesome and widespread grass weed in China. The acetolactate synthase (ALS)-inhibiting herbicide nicosulfuron has been intensively used to manage S. viridis, and this has substantially increased the selection pressure. Here we confirmed a 35.8-fold resistance to nicosulfuron in an S. viridis population (R376 population) from China and characterized the resistance mechanism. Molecular analyses revealed an Asp-376-Glu mutation of the ALS gene in the R376 population. The participation of metabolic resistance in the R376 population was proved by cytochrome P450 monooxygenases (P450) inhibitor pre-treatment and metabolism experiments. To further elucidate the mechanism of metabolic resistance, eighteen genes that could be related to the metabolism of nicosulfuron were obtained bythe RNA sequencing. The results of quantitative real-time PCR validation indicated that three ATP-binding cassette (ABC) transporters (ABE2, ABC15, and ABC15-2), four P450 (C76C2, CYOS, C78A5, and C81Q32), and two UDP-glucosyltransferase (UGT) (UGT13248 and UGT73C3), and one glutathione S-transferases (GST) (GST3) were the major candidates that contributed to metabolic nicosulfuron resistance in S. viridis. However, the specific role of these ten genes in metabolic resistance requires more research. Collectively, ALS gene mutations and enhanced metabolism may be responsible for the resistance of R376 to nicosulfuron.

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A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil

Cited by 6 publications

References 25 publications

Conyza bonariensis’ Resistance to Glyphosate, Diclosulam, and Chlorimuron: Confirmation and Alternative Control for the First Case of Multiple and Cross-Resistance in Uruguay

Conyza bonariensis’ Resistance to Glyphosate, Diclosulam, and Chlorimuron: Confirmation and Alternative Control for the First Case of Multiple and Cross-Resistance in Uruguay

Investigation of Imidazolinone Herbicide Resistance Gene with KASP Markers for Japonica/Geng Rice Varieties in the Huanghuaihai Region of China

Molecular Characterization of Resistance to Nicosulfuron in Setaria viridis

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