Multiple amplified enzyme-free electrochemical immunosensor based on G-quadruplex/hemin functionalized mesoporous silica with redox-active intercalators for microcystin-LR detection

Gan, Cuifen; Wang, Bingfeng; Huang, James; Qileng, Aori; He, Zhiyuan; Lei, Hongtao; Liu, Weipeng; Liu, Yingju

doi:10.1016/j.bios.2017.06.038

Cited by 49 publications

(28 citation statements)

References 38 publications

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“…The TIP3PBOX model was chosen for the solvent model along with the Oct solvent method with a minimum distance of 10 Å to the octa edge. According to our previous similar studies [ 42 ], the energy of the structure was minimized with 10,000 runs and 1 fs for 10 ns simulation time with a simulation temperature of 310 K.…”

Section: Methodsmentioning

confidence: 99%

Production of Antigen-Binding Fragment against O,O-Diethyl Organophosphorus Pesticides and Molecular Dynamics Simulations of Antibody Recognition

Chen

Zhang

Wang

et al. 2018

IJMS

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Immunoassay for pesticides is an emerging analytical method since it is rapid, efficient, sensitive, and inexpensive. In this study, a recombinant antigen-binding fragment (Fab) against a broad set of O,O-diethyl organophosphorus pesticides (DOPs) was produced and characterized. The κ chain and Fd fragment were amplified via PCR and inserted into the vector pComb3XSS and the soluble Fab on phagemid pComb3XSS was induced by isopropyl β-d-thiogalactoside in E. coli TOP 10F’. SDS-PAGE, Western blotting, and indirect competitive ELISA results indicated that Fab maintained the good characteristics of the parental mAb. To better understand antibody recognition, the three-dimensional (3D) model of Fab was built via homologous modeling and the interaction between Fab and DOPs was studied via molecular docking and dynamics simulations. The model clearly explained the interaction manner of Fab and DOPs, and showed that the Arg-L96 and Arg-H52 were mainly responsible for antibody binding. This work provided a foundation for further mutagenesis of Fab to improve its characteristics.

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Section: Methodsmentioning

confidence: 99%

Production of Antigen-Binding Fragment against O,O-Diethyl Organophosphorus Pesticides and Molecular Dynamics Simulations of Antibody Recognition

Chen

Zhang

Wang

et al. 2018

IJMS

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“…Because of their sensitivity and relative simplicity, aptasensing strategies have received great attention for the detection of MC-LR [91]. Gan et al reported a multiple amplified enzyme-free biosensor for MC-LR detection using G-quadruplex/hemin functionalized mesoporous silica with redox-active intercalators [92]. Figure 8 shows the synthesis of monodisperse core-shell mesoporous silica (SiO 2 @MSN)-functionalized DNAzyme concatemers to load hemin and MB as the mimic enzyme.…”

Section: Toxinsmentioning

confidence: 99%

“…Schematic illustration of the preparation of SiO 2 @MSN-NH 2 -MB-Ab 2 -S 0 nano-biomaterial conjugation (A) and the construction of the electrochemical immunosensor for MC-LR (B). Reproduced from[92], with permission.…”

mentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

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“…Thin-layer chromatography, high-performance liquid chromatography, enzyme-linked immunosorbent assay, liquid chromatography/mass spectrometry and protein phosphatase inhibition assays are the techniques generally applied to detect low concentrations of MC-LR [12,13]. Recently, electrochemical immunosensors and label-free fluorescent probes for MC-LR detection have been developed as an alternative to traditional techniques, offering low detection limits, no need for sample pretreatment and easy manipulation [14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Label‐free Immunosensor for the Determination of Microcystin‐LR in Water

2020

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This paper describes the development of a label-free electrochemical immunosensor based on polyethyleneimine-stabilized magnetite nanoparticles (MGNnP-PEI) for the determination of microcystin-LR (MC-LR). MGNnP-PEI was immobilized on the glassy carbon electrode (GCE) surface and employed as a platform for anchorage of the anti-MC-LR. Hydroqui-none in solution was used as a redox probe. After optimization, the immunosensor exhibited a linear response to MC-LR concentrations in the range of 0.1 to 500 ng mL À 1 with a calculated detection limit of 0.053 ng mL À 1. The immunosensor was satisfactorily applied in the quantification of MC-LR in a water sample collected from a freshwater lagoon.

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Multiple amplified enzyme-free electrochemical immunosensor based on G-quadruplex/hemin functionalized mesoporous silica with redox-active intercalators for microcystin-LR detection

Cited by 49 publications

References 38 publications

Production of Antigen-Binding Fragment against O,O-Diethyl Organophosphorus Pesticides and Molecular Dynamics Simulations of Antibody Recognition

Production of Antigen-Binding Fragment against O,O-Diethyl Organophosphorus Pesticides and Molecular Dynamics Simulations of Antibody Recognition

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

Label‐free Immunosensor for the Determination of Microcystin‐LR in Water

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