Label‐free Immunosensor for the Determination of Microcystin‐LR in Water

Abstract

This paper describes the development of a label-free electrochemical immunosensor based on polyethyleneimine-stabilized magnetite nanoparticles (MGNnP-PEI) for the determination of microcystin-LR (MC-LR). MGNnP-PEI was immobilized on the glassy carbon electrode (GCE) surface and employed as a platform for anchorage of the anti-MC-LR. Hydroqui-none in solution was used as a redox probe. After optimization, the immunosensor exhibited a linear response to MC-LR concentrations in the range of 0.1 to 500 ng mL À 1 with a calculated detection limit of 0.053 ng mL À 1. The immunosensor was satisfactorily applied in the quantification of MC-LR in a water sample collected from a freshwater lagoon.

CRISPR-Cas12a-Based Aptasensor for On-Site and Highly Sensitive Detection of Microcystin-LR in Freshwater

“…Enzyme-linked immunosorbent assays (ELISA) are also widely used for MC-LR detection. − However, ELISA need multistep protocols and is lack of sensitivity. Colorimetric and optical sensors for MC-LR detection are ease of operation, which are also limited by the sensitivity or specificity. − Electrochemical-based assays improve the sensitivity and utilize simple equipment, becoming one of the most attractive alternatives for MC-LR monitoring. − Nevertheless, these assays usually demand well-modified electrodes, which suffer from low reproducibility. Thus, detection methods that are easy to operate for on-site testing with high sensitivity and selectivity are still extremely desired.…”