Multigene sequence analysis reveals occurrence of genetic variability in Candidatus Liberibacter asiaticus associated with Huanglongbing of Western and North-Eastern India

Diksha, Damini; Sidharthan, V. Kavi; Singhal, Pankhuri; Choudhary, Nandlal; Sharma, Susheel Kumar; Nabi, Sajad Un

doi:10.1007/s42360-022-00592-8

Cited by 3 publications

(1 citation statement)

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“…Results envisaged that the potential geographical distribution in Eastern Africa encompasses significant citrus-producing areas within Ethiopia, Kenya, and Tanzania, encompassing regions where the disease has yet to be documented. Diksha, et al (2023), took a study to investigate the association between CLas and various citrus cultivars from diverse locations in Western and North-Eastern India by examining the genetic diversity and interrelationships among isolates through the characterization of different genetic markers. The results of the phylogenetic analysis conducted on CLas isolates utilizing rplA-rplJ gene sequences revealed the presence of two distinct clusters.…”

Section: Figure 1hlb-infected Tree With Symptoms Of Blotchy Mottling ...mentioning

confidence: 99%

Decoding Huanglongbing: Understanding the Origins, Impacts, and Remedies for Citrus Greening

Usman,

Saleem,

Shafique

2023

Phytopathogenomics and Disease Control

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Citrus cultivation is a vital component of the agricultural sector, offering significant nutritional and economic benefits. However, citrus plants face numerous disorders, including Huanglongbing (HLB), also known as citrus greening disease. HLB is a devastating disease caused by the bacterial species Candidatus Liberibacter asiaticus (Las), primarily transmitted by the citrus psyllid and grafting. Investigating the distribution and molecular characterization of HLB in various citrus varieties and psyllid populations is crucial. Molecular techniques and iodine starch staining have been used to detect HLB symptoms. Several studies have been designed to determine the incidence and severity of HLB symptoms in different citrus varieties and locations and estimate the psyllid population density in different citrus varieties. Furthermore, these studies established a correlation between the population of psyllids and the incidence and severity of HLB in different locations and these findings suggest a potential link between psyllid infestation and the prevalence of HLB. This study highlights the prevalence of HLB in various locations and varieties, providing insights into the distribution, severity, and correlation between psyllid populations and HLB incidence. The reliability of PCR testing for Las infection confirms its effectiveness. Such findings suggest implementing integrated pest management strategies, including monitoring and controlling psyllid populations, removing infected plants, and using certified disease-free planting materials to prevent HLB spread.

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Section: Figure 1hlb-infected Tree With Symptoms Of Blotchy Mottling ...mentioning

confidence: 99%

Decoding Huanglongbing: Understanding the Origins, Impacts, and Remedies for Citrus Greening

Usman,

Saleem,

Shafique

2023

Phytopathogenomics and Disease Control

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Identification of two putative novel deltapartitiviruses and an enamovirus in coriander transcriptomes

Kavi Sidharthan,

Diksha,

Singh

et al. 2023

Arch Microbiol

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Coriander is a herbaceous spice and condiment crop also known for its medicinal properties. The present study identi ed two putative novel deltapartitiviruses and an enamovirus tentatively named as Coriandrum sativum deltapartitivirus 1, 2 (CsDPV1, 2) and Coriandrum sativum enamovirus (CsEV) in the publicly available transcriptome-assembled contigs derived from coriander grown in India. CsDPV1 and 2 contained tripartite and bipartite genomes, respectively with each genome segment encoding for a single ORF. CsEV contained ve ORFs encoding for proteins P0, P1, P1-2, P3 and P3-5. Phylogenetic analysis revealed three distinct subgroups of deltapartitiviruses wherein CsDPV1 and 2 grouped in subgroup 3 and 1, respectively while CsEV formed a distinct sub-clade within enamoviruses. Further, presence of CsDPV2 in fruit samples of one of the cultivars from where the virus was identi ed was validated through RT-PCR assay and Sanger sequencing. The study highlights the need for further studies on understanding the importance and biological properties of identi ed novel viruses. Full TextCoriandrum sativum L., commonly known as coriander, is an economically important herbaceous spice crop belonging to the family Apiaceae. Though native to the Mediterranean and Middle East, the crop is widely cultivated in different regions of the world.

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Identification of two putative novel deltapartitiviruses and an enamovirus in coriander transcriptomes

Sidharthan

Diksha

Singh

et al. 2023

Preprint

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Coriander is a herbaceous spice and condiment crop also known for its medicinal properties. The present study identified two putative novel deltapartitiviruses and an enamovirus tentatively named as Coriandrum sativum deltapartitivirus 1, 2 (CsDPV1, 2) and Coriandrum sativum enamovirus (CsEV) in the publicly available transcriptome-assembled contigs derived from coriander grown in India. CsDPV1 and 2 contained tripartite and bipartite genomes, respectively with each genome segment encoding for a single ORF. CsEV contained five ORFs encoding for proteins P0, P1, P1–2, P3 and P3–5. Phylogenetic analysis revealed three distinct subgroups of deltapartitiviruses wherein CsDPV1 and 2 grouped in subgroup 3 and 1, respectively while CsEV formed a distinct sub-clade within enamoviruses. Further, presence of CsDPV2 in fruit samples of one of the cultivars from where the virus was identified was validated through RT-PCR assay and Sanger sequencing. The study highlights the need for further studies on understanding the importance and biological properties of identified novel viruses.

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Multigene sequence analysis reveals occurrence of genetic variability in Candidatus Liberibacter asiaticus associated with Huanglongbing of Western and North-Eastern India

Cited by 3 publications

References 46 publications

Decoding Huanglongbing: Understanding the Origins, Impacts, and Remedies for Citrus Greening

Decoding Huanglongbing: Understanding the Origins, Impacts, and Remedies for Citrus Greening

Identification of two putative novel deltapartitiviruses and an enamovirus in coriander transcriptomes

Identification of two putative novel deltapartitiviruses and an enamovirus in coriander transcriptomes

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