Identification of two putative novel deltapartitiviruses and an enamovirus in coriander transcriptomes

Abstract

Coriander is a herbaceous spice and condiment crop also known for its medicinal properties. The present study identi ed two putative novel deltapartitiviruses and an enamovirus tentatively named as Coriandrum sativum deltapartitivirus 1, 2 (CsDPV1, 2) and Coriandrum sativum enamovirus (CsEV) in the publicly available transcriptome-assembled contigs derived from coriander grown in India. CsDPV1 and 2 contained tripartite and bipartite genomes, respectively with each genome segment encoding for a single ORF. CsEV contained ve ORFs encoding for proteins P0, P1, P1-2, P3 and P3-5. Phylogenetic analysis revealed three distinct subgroups of deltapartitiviruses wherein CsDPV1 and 2 grouped in subgroup 3 and 1, respectively while CsEV formed a distinct sub-clade within enamoviruses. Further, presence of CsDPV2 in fruit samples of one of the cultivars from where the virus was identi ed was validated through RT-PCR assay and Sanger sequencing. The study highlights the need for further studies on understanding the importance and biological properties of identi ed novel viruses. Full TextCoriandrum sativum L., commonly known as coriander, is an economically important herbaceous spice crop belonging to the family Apiaceae. Though native to the Mediterranean and Middle East, the crop is widely cultivated in different regions of the world.