Identification of two putative novel deltapartitiviruses and an enamovirus in coriander transcriptomes

Abstract

Coriander is a herbaceous spice and condiment crop also known for its medicinal properties. The present study identified two putative novel deltapartitiviruses and an enamovirus tentatively named as Coriandrum sativum deltapartitivirus 1, 2 (CsDPV1, 2) and Coriandrum sativum enamovirus (CsEV) in the publicly available transcriptome-assembled contigs derived from coriander grown in India. CsDPV1 and 2 contained tripartite and bipartite genomes, respectively with each genome segment encoding for a single ORF. CsEV contained five ORFs encoding for proteins P0, P1, P1–2, P3 and P3–5. Phylogenetic analysis revealed three distinct subgroups of deltapartitiviruses wherein CsDPV1 and 2 grouped in subgroup 3 and 1, respectively while CsEV formed a distinct sub-clade within enamoviruses. Further, presence of CsDPV2 in fruit samples of one of the cultivars from where the virus was identified was validated through RT-PCR assay and Sanger sequencing. The study highlights the need for further studies on understanding the importance and biological properties of identified novel viruses.