Multigene differences between<i>Microdochium nivale</i>and<i>Microdochium majus</i>

Jewell, Linda Elizabeth; HsiangTom,

doi:10.1139/cjb-2012-0178

Cited by 34 publications

(36 citation statements)

References 30 publications

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“…Intriguingly, the mating type (MAT) genes and reproductive strategy in Microdochium fungi have not yet been completely elucidated; hence, the structure of the mating type locus and its surrounding regions has been under intensive focus [53,55]. To identify MAT genes within Microdochium spp.…”

Section: Discussionmentioning

confidence: 99%

“…The primers V9G [25] and ITS4 [26] were used to amplify part (ITS) of the nuclear rDNA operon spanning the 3 end of the 18S rRNA gene, the first internal transcribed spacer (ITS1), the 5.8S rRNA gene, and the second ITS region (ITS2). Part of the beta-tubulin gene region (BTUB) was amplified using primers Btub526F and Btub1332R [27], and for the RNA polymerase II second largest subunit gene (RPB2) primers RPB150F [27] and fRPB2-7cR [28] were used. Amplification of LSU and ITS regions was performed as described previously [29].…”

Section: Microdochium Strainsmentioning

confidence: 99%

“…Amplification of LSU and ITS regions was performed as described previously [29]. The conditions for BTUB and RPB2 followed Jewell and Hsiang [27]. The sequencing of the fragments was carried out on an ABI Prism 3500 sequencer (Applied Biosystems, Hitachi, Japan) using the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).…”

Section: Microdochium Strainsmentioning

confidence: 99%

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Evidence of Microdochium Fungi Associated with Cereal Grains in Russia

et al. 2020

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In total, 46 Microdochium strains from five different geographic regions of Russia were explored with respect to genetic diversity, morphology, and secondary metabolites. Based on the results of PCR, 59% and 28% of the strains were identified as M. nivale and M. majus, respectively. As a result of sequencing four genome regions, namely ITS, LSU, BTUB, and RPB2 (2778 bp), five genetically and phenotypically similar strains from Western Siberia were identified as M. seminicola, which, according to our findings, is the prevalent Microdochium species in this territory. This is the first record of M. seminicola in Russia. Attempts were made to distinguish between Microdochium species and to identify species-specific morphological characteristics in the anamorph and teleomorph stages and physiological properties. We examined the occurrence frequency of conidia with different numbers of septa in the strains of Microdochium. The predominance of three-septate macroconidia in M. majus was higher than that in M. nivale and typically exceeded 60% occurrence. Most M. majus and M. nivale strains formed walled protoperithecia on wheat stems. Only three strains of M. majus and one strain each of M. nivale and M. seminicola produced mature perithecia. The growth rate of M. seminicola strains was significantly lower on agar media at 5–25 °C than those of M. majus and M. nivale strains. Multimycotoxin analysis by HPLC-MS/MS revealed that the strains of three Microdochium species did not produce any toxic metabolites.

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Section: Discussionmentioning

confidence: 99%

Section: Microdochium Strainsmentioning

confidence: 99%

Section: Microdochium Strainsmentioning

confidence: 99%

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Evidence of Microdochium Fungi Associated with Cereal Grains in Russia

et al. 2020

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“…Based on the ISSR data, there is a distinct resolution between M. majus and M. nivale as in the previous reports (Glynn et al 2005;Jewell and Hsiang 2013), and each of all 172 isolates from the five populations was identified as one of eight ISSR haplotypes of M. majus or six ISSR haplotypes of M. nivale. The Tokachi population showed the highest haplotype diversity, followed by Sorachi and Abashiri (Table 3).…”

Section: Discussionmentioning

confidence: 71%

“…majus exhibits a high level of genetic uniformity, indicating that this variety generally reproduces homothallically in nature (Lees et al 1995). Analysis of the DNA sequences of RNA polymerase II (RPB2), β-tubulin, and elongation factor from these varieties supported the recent elevation of M. nivale and M. majus to sister species (Jewell and Hsiang 2013). The RPB2 sequences also resolved both the North American and European M. majus isolates and M. nivale isolates from either turfgrasses or wheat into separate clades.…”

Section: Introductionmentioning

confidence: 89%

Population genetic structure of Microdochium majus and Microdochium nivale associated with Fusarium head blight of wheat in Hokkaido, Japan

et al. 2014

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Microdochium majus and Microdochium nivale are two of fungal pathogens that cause Fusarium head blight (FHB) in wheat, and have also caused pink snow mold in eastern Hokkaido, Japan. With the aim of assessing levels of genetic variation and population structure, 172 isolates of these Microdochium species obtained from five populations of infected wheat seeds were first classified into each species using polymerase chain reaction (PCR) amplification with specific primers. In total 165 (95.9 % of all isolates) and seven isolates (six of Tokachi populations and one of Abashiri populations) were identified as M. majus and M. nivale, respectively, indicating that M. majus was predominant and the main causal pathogen of FHB in this area. Inter-simple sequence repeat (ISSR) analysis showed that the total genetic diversity was 0.023 when estimated by Nei's gene diversity index within the five populations dominated by M. majus. An AMOVA analysis also showed that 86.74 % of the total genetic variation was within populations and 13.26 % among populations. These results indicated that little genetic differentiation occurred among the five populations of M. majus. Based on the unweighted pair group method of cluster analysis using the ISSR data, all isolates were identified as one of eight haplotypes in M. majus or six haplotypes in M. nivale, allowing the construction of a dendrogram with two clades corresponding to each species. There was no correlation between the clustering of isolates and their geographic distribution on the tree. These findings show that migration is likely playing an important role in the population biology of M. majus, providing some support for the prediction of epidemics of fungicide resistant strains within populations of the FHB pathogen

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Diversity of Physiological and Biochemical Characters of Microdochium Fungi

Gavrilova

Орина

Kessenikh

et al. 2020

Chemistry & Biodiversity

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The biological characterization of Microdochium majus, M. nivale, and M. seminicola strains with wide geographical origins showed the diversity of their pathogenic properties and metabolite compounds, allowing them to exist in their habitats. Significant differences in the ability of Microdochium fungi to cause lesions on wheat and oat leaves were found. The intensity of symptoms depended on the species and substrate origin of the strains. On average M. seminicola strains were able to cause less leaf necrosis than M. majus and M. nivale. The volatile organic compound (VOC) profile of Microdochium fungi included 29 putative fungal metabolites. The spectrum of the identified VOCs in M. seminicola strains was much richer than that in M. majus and M. nivale strains. In addition, the strains of M. seminicola emitted at least six sesquiterpenes. Mycotoxin analysis by HPLC/MS/MS revealed that the analyzed Microdochium strains did not produce any toxic metabolites typically produced by filamentous fungi.

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Multigene differences betweenMicrodochium nivaleandMicrodochium majus

Cited by 34 publications

References 30 publications

Evidence of Microdochium Fungi Associated with Cereal Grains in Russia

Evidence of Microdochium Fungi Associated with Cereal Grains in Russia

Population genetic structure of Microdochium majus and Microdochium nivale associated with Fusarium head blight of wheat in Hokkaido, Japan

Diversity of Physiological and Biochemical Characters of Microdochium Fungi

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