Multifunctional polyplex micelles for efficient microRNA delivery and accelerated osteogenesis

Abstract

MicroRNAs (miRNAs) are emerging as a novel class of molecular targets and therapeutics to control gene expression for tissue repair and regeneration. However, a safe and effective transfection of miRNAs...

Delivery process and effective design of vectors for cancer therapy

“…3,42 Therefore, the combined therapy of gene and drug control to inhibit tumor cells and avoid drug immunity caused by multiple-dose administration has become a new research direction. 42,43 This method places high requirements on the carrier material. It needs to load anticancer drugs and target genes at the same time without interfering with each other so that the drugs and genes can be effectively delivered to target cells and released and expressed.…”

MicroRNA-loaded biomaterials for osteogenesis

“…Long-term and stable nucleic acid carrying capacity is difficult to obtain Frontiers in Bioengineering and Biotechnology frontiersin.org by surface coating. Therefore, it is necessary to combine other materials to develop more efficient biological coatings or make metal scaffolds with electric charges to bind to miRNA through electrostatic adsorption (Yu et al, 2017;Li et al, 2021a). Ti metallic materials have been widely studied in the field of bone regeneration medicine, owing to their excellent loadbearing stability and biocompatibility (Sun et al, 2020b;Liu et al, 2021b).…”

“…Novel organic nanobiomaterials developed in recent years are regarded as reliable carriers, owing to their high transfection efficiency and cell innocuity. For example, Li et al (2021a) reported a study using low molecular weight protamine (LMWP) rich in arginine as the carrier of the nucleic acid molecule miR-29b. However, due to the unstable structure of LMWP and low lysosomal escape that affects delivery efficiency, new stable organic carriers must be constructed.…”

“…The defect area showed strong nerve and its innervated bone regeneration. Qian et al ( Li et al, 2021a ) designed amphiphilic carriers by combining a hydrophobic PLGA segment with a hydrophilic PEG segment and then inserting PEI with the function of escape lysosome degradation and R9-G4-IKVAVW (RGI) peptide with the ability to recognize BMSCs and penetrate the cell membrane to form a new nucleic acid carrier for carrying miR-218 targeting the negative regulator of the Wnt/β-catenin signaling pathway. Li et al (2020) constructed 3D nanofiber aerogels using PLGA-collagen-gelatin (PCG) and bioactive glass (BG) nanofibers, carrying a miR-26a delivery vector consisting of HA-disulfide-ethanolamine-functionalized poly (glycerol methacrylate).…”

“…Long-term and stable nucleic acid carrying capacity is difficult to obtain by surface coating. Therefore, it is necessary to combine other materials to develop more efficient biological coatings or make metal scaffolds with electric charges to bind to miRNA through electrostatic adsorption ( Yu et al, 2017 ; Li et al, 2021a ).…”