Multifunctional Mn-containing titania coatings with enhanced corrosion resistance, osteogenesis and antibacterial activity

Yu, Le; Shi, Qian; Qiao, Yan; Liu, Xuanyong

doi:10.1039/c4tb00594e

Cited by 52 publications

(38 citation statements)

References 66 publications

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“…To solve these problems, multiple strategies have been developed to obtain surfaces that are able to reduce the risk of bacterial contamination and promote cell proliferation [2,13]. Introduction of inorganic antibacterial agents into HAP 3 coating has achieved growing attention [2,14,15]. In recent years, incorporation of metallic antibacterial agents (such as Cu 2+ , Ag + , Ce 4+ and Zn 2+ ) in bioceramics is mainly implemented because of their antibacterial property, which aids in inhibiting microbial growth at the implant site, and their lack of cytotoxicity at low concentrations [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Osteoblastic cell responses and antibacterial efficacy of Cu/Zn co-substituted hydroxyapatite coatings on pure titanium using electrodeposition method

et al. 2015

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Effective physiological bone integration and absence of bacterial infection are essential for a successful orthopaedic or dental implant. This work elucidated the antibacterial efficacy and cytocompatibility of electroplated Cu(II) and Zn(II) co-substituted hydroxyapatite (HAP) (i.e., ZnCuHAP) coating on commercially pure titanium (Ti-cp). To improve the antibacterial property of pure HAP, Cu 2+ was substituted into its structure.Simultaneously, Zn 2+ is co-substituted as secondary material into CuHAP to offset the potential cytotoxicity of Cu, because elevated Cu concentration is toxic. The as-deposited coatings were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy and energy-dispersive X-ray spectroscopy. Co-doping of Zn 2+ and Cu 2+ into HAP reduced the porosity, resulting in a denser coating. The Zn 2+ and Cu 2+ ions were homogenously co-deposited into HAP films. Potentiodynamic polarisation test revealed 2 that the ZnCuHAP covered coating provided good barrier characteristics and achieved superior corrosion protection for Ti substrates. The as-prepared ZnCuHAP coating was found to be highly effective against Escherichia coli in vitro. In vitro biocompatibility tests and MTT were employed to assess the cytotoxicity of ZnCuHAP coating with osteoblast-like MC3T3-E1 cells. No adverse effect or cytotoxicity on osteoblasts by Zn/Cu addition was observed, revealing that the co-substitution of Zn in CuHAP efficiently offsets the adverse effects of Cu and improves the performance compared with that of pure HAP. 15 comparison with the HAP coating and the control. Antimicrobial results revealed that Cu ions released from ZnCuHAP present a strong bactericidal effect against these organisms (antimicrobial ratio > 95%). This enhancement may be due to the high release of copper in ZnCuHAP in the PBS medium after 24 h incubation (based on the ICP-AES analysis).The antibacterial tests results revealed that pure HAP coating has no antibacterial property.The pure HAP plate showed more colonies than the control sample (Fig. 6). This finding showed that proteins, amino acids, and other organic matter are readily adsorbed on the HAP surface, which could favour adsorption and replication of bacteria on the coating, usually causing implant-related infections [37]. Therefore, endowing antimicrobial properties to HAP is necessary. Several recent studies have introduced antimicrobial activity of materials that involve Cu(II) ions. Du et al. [38] have suggested that chitosan nanoparticles loaded with Cu(II) ions interact with bacterial cell membranes of E. coli, causing structural changes and, eventually, cell death. Nan et al. [39] also suggested that structure of the outer cell membrane responsible for cell permeability is significantly altered for E. coli after contact with antimicrobial stainless steel with Cu(II) ions. This finding indicates that cell walls were badly undermined, and numerous cellular contents are released. Notably, Zn ions released fromZnCuHAP may also exhi...

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Section: Introductionmentioning

confidence: 99%

Osteoblastic cell responses and antibacterial efficacy of Cu/Zn co-substituted hydroxyapatite coatings on pure titanium using electrodeposition method

et al. 2015

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“…While after Mn-PIII&D, the nano-grains disappeared and the surfaces of Mn-containing samples ( Fig. 1e and 1f within first 24 h, which were 0.0073 mg/L (ppm) and 0.01 mg/L respectively, are much lower than those from the Mn-doped Ti synthesized by MAO technique in our previous study that caused adverse ALP activity and from the nanostructured Mn-doping Ti that caused obvious in vitro disadvantages on cell, which were 0.48 mg/L and 1.65 mg/L, respectively [4,13]. as-etched Ti and Mn8 that contained relatively higher Mn content for 7 and 14 days showed no statistic differences either ( Fig.…”

Section: Surface Characterization and Mn Releasementioning

confidence: 66%

“…Increasing research has focused on surface modification of the most wildly used Ti-based orthopedic materials [2,3]. Among which, our group has paid considerable attention on the surface loading of inorganic additives such as Mn [4], Fe [5], Cu [6], Zn [7,8], Ag [9], or combined dual elements [10,11] on Ti to promote the osteogenic ability and better compromise the cytotoxicity and antibacterial activities of the additives using various techniques. One suggestion in common from these researches is that the biological response to material depends more or less on the concentration, existence form, and stability of the additives.…”

Section: Introductionmentioning

confidence: 99%

“…Particularly, the coatings with reasonable incorporation of Mn that synthesized through Micro Arc Oxidation (MAO) technique on Ti showed good biological performance overall. Whereas adverse rat bone marrow mesenchymal stem cells (rBMMSC) proliferation rate and ALP expression were still observed from the surface containing relatively high dosage of Mn [4]. In addition, there are plenty researches verified that biomaterials overdosed by Mn could be detrimental to cells.…”

Section: Introductionmentioning

confidence: 99%

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Mn-containing titanium surface with favorable osteogenic and antimicrobial functions synthesized by PIII&D

Tian

Qiao

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Reasonable incorporation of manganese into titanium is believed to be able to enhance the osteogenic and antibacterial activities of orthopedic implants. However, it is still a challenge to compromise Mn-induced cytotoxicity and better develop its biocompatibility and antimicrobial ability. To pinpoint this issue, a stable Mn ion release platform was created on Ti using plasma immersion ion implantation and deposition (PIII&D) technique. Compared with as-etched titanium, as a result, promoted antibacterial abilities against gram-negative bacteria species and enhanced osteogenic-related gene expressions on rBMMSC were observed on Mn-containing sample. Meanwhile, the Mn-containing samples showed no obvious cytotoxicity. Our results here provide insight to be better understanding the relationships between additives-induced biological performance and the dose, state, and stability of the doped element.

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“…The Mn was incorporated through the addition of KMnO 4 in PEO electrolyte. The slow release rate of Mn due to high compactness of PEO coating is capable of maintaining antibacterial behavior of coating up to 6 months . Higher Mn concentrations are also found to be cytotoxic, several researchers have confirmed this effect .…”

Section: Antibacterial Coatings Through Peomentioning

confidence: 95%

Surface modification of valve metals using plasma electrolytic oxidation for antibacterial applications: A review

Rizwan

Alias

Zaidi

et al. 2017

J Biomedical Materials Res

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Plasma electrolytic oxidation (PEO) is an advance technique to develop porous oxidation layer on light metals, primarily to enhance corrosion and wear resistance. The oxidation layer can also offer a wide variety of mechanical, biomedical, tribological, and antibacterial properties through the incorporation of several ions and particles. Due to the increasing need of antimicrobial surfaces for biomedical implants, antibacterial PEO coatings have been developed through the incorporation of antibacterial agents. Metallic nanoparticles that have been employed most widely as antibacterial agents are reported to demonstrate serious health and environmental threats. To overcome the current limitations of these coatings, there is a significant need to develop antibacterial surfaces that are not harmful for patient's health and environment. Attention of the readers has been directed to utilize bioactive glasses as antibacterial agents for PEO coatings. Bioactive glasses are well known for their excellent bioactivity, biocompatibility, and antibacterial character. PEO coatings incorporated with bioactive glasses can provide environment-friendly antimicrobial surfaces with exceptional bioactivity, biocompatibility, and osseointegration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 590-605, 2018.

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Multifunctional Mn-containing titania coatings with enhanced corrosion resistance, osteogenesis and antibacterial activity

Cited by 52 publications

References 66 publications

Osteoblastic cell responses and antibacterial efficacy of Cu/Zn co-substituted hydroxyapatite coatings on pure titanium using electrodeposition method

Osteoblastic cell responses and antibacterial efficacy of Cu/Zn co-substituted hydroxyapatite coatings on pure titanium using electrodeposition method

Mn-containing titanium surface with favorable osteogenic and antimicrobial functions synthesized by PIII&D

Surface modification of valve metals using plasma electrolytic oxidation for antibacterial applications: A review

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