Multidimensional counter-current chromatographic system and its application

Yang, Fuchao; Jiang, Quan; Zhang, Tian You; Ito, Yoichiro

doi:10.1016/s0021-9673(97)01273-9

Cited by 68 publications

(39 citation statements)

References 5 publications

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“…The solvent system, consisting in chromatography grade chloroform, methanol, and water (4/3/2 v/v/v), already used to separate Ginkgo biloba and Hippophae rhamnoides flavonoids using high-speed counter-current chromatography [36] was hence selected as the one that gave suitable Kd and α values for an optimized RL3-MeOH sub-extract's fractionation. A total of 90 tubes were collected and gathered together following the CPC chromatogram (Supplementary data 2) to obtain 18 fractions (named RL3-F1 to RL3-F18 respectively), the whitening activities of which were again tested (RL3-F1 actually corresponding to the CPC solvent dead space was not tested for bioactivity; Figure 3).…”

Section: Final Purification and Identification Of Active Compoundsmentioning

confidence: 99%

Whitening Agents from Reseda luteola L. and Their Chemical Characterization Using Combination of CPC, UPLC-HRMS and NMR

et al. 2017

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Skin whitening agents occupy an important part of the dermo-cosmetic market nowadays. They are used to treat various skin pigmentation disorders, or simply to obtain a lighter skin tone. The use of traditional skin bleachers (e.g., hydroquinone, corticoids) is now strictly regulated due to their side effects. When considering this and the growing consumers' interest for more natural ingredients, plant extracts can be seen as safe and natural alternatives. In this perspective, in vitro bioassays were undertaken to assess cosmetic potential of Reseda luteola, and particularly its promising whitening activities. A bioguided purification procedure employing centrifugal partition chromatography, Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-HRMS) and NMR was developed to isolate and identify the whitening agents (i.e., luteolin and apigenin) from aerial parts of R. luteola. UPLC-HRMS also enabled the characterization of acetylated luteolin-and apigenin-O-glycosides, which occurrence is reported for the first time in R. luteola.

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Section: Final Purification and Identification Of Active Compoundsmentioning

confidence: 99%

Whitening Agents from Reseda luteola L. and Their Chemical Characterization Using Combination of CPC, UPLC-HRMS and NMR

et al. 2017

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“…The total peak capacity of the comprehensive 2-D system could be as high as 2000. Yang et al [65] developed a multi-dimensional counter-current chromatographic system and used it for the preparative separation of isorhamnetin, kaempferol, and quercetin from crude flavone aglycones of Ginkgo biloba L. and Hippophae rhamnoides L. Sheng et al [66] developed a 2-D-LC/MS for analysis of the matrine alkaloids. Five alkaloids were detected and five compounds were detected from the gangliosides.…”

Section: Multidimensional Separation Modes For Traditional Chinese Mementioning

confidence: 99%

Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines

Gao

Deng

Lin

et al. 2007

J of Separation Science

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ReviewRecent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicinesThe most basic task in proteomics remains the detection and identification of proteins from a biological sample, and the most traditional way to achieve this goal consists in protein separations performed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Yet the 2-D PAGE-mass spectrometry (MS) approach has its drawbacks with regard to automation, sensitivity, and throughput. Consequently, considerable effort has been devoted to the development of non-gel-based proteome separation technologies in an effort to alleviate the shortcomings of 2-D PAGE. In addition, traditional Chinese medicines (TCMs), due to their long period of clinical testing and reliable therapeutic efficacy, are attracting increased global attention. However, hundreds or even thousands of components are usually present in TCMs, which results in great difficulties of separation. As a mainstream separation tool, multidimensional liquid separation systems have shown powerful separation ability, high peak capacity, and excellent detectability in the analysis of complex samples including biological samples and TCMs, etc. Therefore, this review emphasizes the most recent advances in multidimensional liquid chromatography and capillary electrophoresis-based separation techniques, and the corresponding applications in proteomics and TCMs. In view of the significant contributions from Chinese scientists, this review focuses mainly on the work of Chinese scientists in the above fields.

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“…Although HPLC is mostly used for analysing the constituents of G. biloba [12,13], the use of HPLC for the analysis of traditional Chinese medicines often has many shortcomings such as long analysis time, low resolution and short column lifetime due to easy contamination. TLC [14], GC [15,16] and CE [17,18] have also been used for this purpose.…”

Section: Introductionmentioning

confidence: 99%

A new capillary zone electrophoresis method for the analysis of Ginkgo biloba extracts and Ginkgo biloba pharmaceutical products

Shaban¹,

Gajdošová

Havel

2006

J of Separation Science

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Ginkgo biloba, traditional Chinese medicine is now generally accepted. Separation and determination of active components in G. biloba is important for the product quality control. Therefore, the development of an effective and reliable separation method is important. In this work, a new capillary electrophoretic (CZE) method for separation of the G. biloba leaf extracts components was developed and optimized by the use of experimental design and artificial neural network (ANN). Under best separation conditions, in gamma-CD-modified buffer, the separation was reached within 10 min (36 mM borate BGE, pH 9.2, 1 mM gamma-CD), while the hydrodynamic mode for sample injection (2 s) and UV detection at 270 nm were applied. The method developed was validated and applied for analysis of various extracts and G. biloba products.

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Multidimensional counter-current chromatographic system and its application

Cited by 68 publications

References 5 publications

Whitening Agents from Reseda luteola L. and Their Chemical Characterization Using Combination of CPC, UPLC-HRMS and NMR

Whitening Agents from Reseda luteola L. and Their Chemical Characterization Using Combination of CPC, UPLC-HRMS and NMR

Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines

A new capillary zone electrophoresis method for the analysis of Ginkgo biloba extracts and Ginkgo biloba pharmaceutical products

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