Whitening Agents from Reseda luteola L. and Their Chemical Characterization Using Combination of CPC, UPLC-HRMS and NMR

Abstract

Skin whitening agents occupy an important part of the dermo-cosmetic market nowadays. They are used to treat various skin pigmentation disorders, or simply to obtain a lighter skin tone. The use of traditional skin bleachers (e.g., hydroquinone, corticoids) is now strictly regulated due to their side effects. When considering this and the growing consumers' interest for more natural ingredients, plant extracts can be seen as safe and natural alternatives. In this perspective, in vitro bioassays were undertaken to assess cosmetic potential of Reseda luteola, and particularly its promising whitening activities. A bioguided purification procedure employing centrifugal partition chromatography, Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-HRMS) and NMR was developed to isolate and identify the whitening agents (i.e., luteolin and apigenin) from aerial parts of R. luteola. UPLC-HRMS also enabled the characterization of acetylated luteolin-and apigenin-O-glycosides, which occurrence is reported for the first time in R. luteola.

Microextraction of Reseda luteola-Dyed Wool and Qualitative Analysis of Its Flavones by UHPLC-UV, NMR and MS

“…The most detailed studies so far are those by Moiteiro et al, Marques et al,Mantzouris et al and Otłowska et al [24,26,28,34]. In contrast, the study by Burger is confusing with the major peaks reported as unknown [35]. Further, it is unclear where exactly luteolin-4 -O-glucoside elutes.…”

EVALUACIÓN DE EXTRACTOS VEGETALES COMO UNA ALTERNATIVA DE CONTROL PARA DOS PLAGAS DE MAÍZ ALMACENADO, Sitophilus zeamais (Motschulsky) y Prostephanus truncatus (Horn)

“…Para S. zeamais, también presentaron buenos resultados los extractos en alcohol de N. glauca, P. clandestinum y R. luteola, al ocasionar más del 42 % de mortalidad en este insecto. Este efecto de la Gualda (R. luteola) está relacionado con sus metabolitos secundarios que contienen azufre y nitrógeno (Burger et al 2017). En P. truncatus, dos de los extractos en alcohol, presentaron resultados sobresalientes al ocasionar una mortalidad de 62.5 % (R. luteola) y 50 % (S. ballotiflora); así como los extractos alcohólicos de N. glauca y P. clandestinum, que causaron más del 40 % de mortalidad (Tabla 2).…”

“…For S. zeamais, alcohol extracts of N. glauca, P. clandestinum, and R. luteola also presented worthy performance, causing more than 42 % mortality in this insect. This effect of Gualda (R. luteola) is related to its secondary metabolites containing sulfur and nitrogen (Burger et al 2017). In P. truncatus, two of the extracts in alcohol presented outstanding results by causing mortality of 62.5 % (R. luteola) and 50 % (S. ballotiflora); as well as the alcoholic extracts of N. glauca and P. clandestinum, which caused more than 40 % mortality (Table 2).…”