A new capillary zone electrophoresis method for the analysis of Ginkgo biloba extracts and Ginkgo biloba pharmaceutical products

Abstract

Ginkgo biloba, traditional Chinese medicine is now generally accepted. Separation and determination of active components in G. biloba is important for the product quality control. Therefore, the development of an effective and reliable separation method is important. In this work, a new capillary electrophoretic (CZE) method for separation of the G. biloba leaf extracts components was developed and optimized by the use of experimental design and artificial neural network (ANN). Under best separation conditions, in gamma-CD-modified buffer, the separation was reached within 10 min (36 mM borate BGE, pH 9.2, 1 mM gamma-CD), while the hydrodynamic mode for sample injection (2 s) and UV detection at 270 nm were applied. The method developed was validated and applied for analysis of various extracts and G. biloba products.

Development and validation of an ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry method for the simultaneous determination of selected flavonoids in Ginkgo biloba

“…Various analytical methods including HPLC with photodiode array detection (PDA), HPLC with evaporative light scattering detection (ELSD), LC–UV–MS, HPLC–MS/MS, UHPLC–PDA, RP high‐performance TLC, 1 H NMR, GC with flame ionization detection, mixed micellar EKC, CZE, HILIC‐UHPLC–ESI‐MS/MS, and UHPLC–ESI‐MS/MS have been reported for the quantitative determination of flavonoids, terpene lactones, and underivatized amino acids in Ginkgo leaf extract and marketed herbal formulations . Although HPLC–ELSD and HPLC–PDA methods have been reported to study variations of the flavonol glycosides at different ages, different cultivation sources, and genders of G. biloba leaves but variations of flavans, flavonol glycosides, flavonols, flavones, isoflavone, and biflavones in the leaf, stem, and fruit extracts of male and female trees of G. biloba using UHPLC–ESI‐MS/MS in multiple reaction monitoring (MRM) mode have still not been reported.…”

Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule

“…Thus, the selection of a suitable method to optimize the separation conditions is important. There are many approaches available for the optimization of separation conditions, such as experimental design coupled with response surface method, multivariate analysis, or artificial neural network method (Ben Hameda et al, 2006;Lin, 2006;Shaban et al, 2006;Hanrahan et al, 2008;Liu et al, 2009). Central composite design (CCD), one of experimental design methods, has been widely used to optimize the separation conditions of CE, owing to its simple procedure, fewer experimental runs, and its advantage in the full evaluation on interactions of factors (Lin, 2006;Liu et al, 2006;Yu et al, 2006a;2006b;2006c;2007;Liu et al, 2008).…”

Recent Applications of Capillary Electrophoresis in the Analysis of Traditional Chinese Medicines

“…Wu et T.L. Ming leaves [20], Ginkgo biloba leaves [21,22], Achillea millefolium [23], Sang-Xing Decoction (a TCM formulation) [24] and Stamen nelumbinis [25]. Herba Epimedii (family Berberidaceae) is commonly used in the treatment of cardiovascular diseases and other chronic illness such as infertility, amnesia and asthenia, impotence and senile functional diseases in China.…”