Multicomponent pattern and biological activities of sevenAsphodelinetaxa: potential sources of natural-functional ingredients for bioactive formulations

Abstract

The current study was carried out to evaluate multicomponent pattern, biological and enzymatic activities of seven Asphodeline taxa root extracts as useful ingredients, due to the fact that these plants are commonly used as traditional food supplements in Turkish regions. The extracts were characterized for free anthraquinones and phenolics to obtain a specific chemical fingerprint useful for quality control. These analyzes were coupled to biological and enzymatic activities in order to obtain comprehensive information of the natural product. Free anthraquinones and phenolics were determined using validated HPLC-PDA methods. Antioxidant properties were determined by different procedures including free radical scavenging, reducing power, phosphomolybdenum and metal chelating assays. Ames assay was performed to evaluate mutagenic/antimutagenic properties. Enzyme inhibitory activities were tested against cholinesterase, tyrosinase, α-amylase and α-glucosidase. From the herein reported results, Asphodeline could be valuable for the production of bioactive products or food supplements for cosmetic and pharmaceutical industries.

Protective Effects Induced by Microwave-Assisted Aqueous Harpagophytum Extract on Rat Cortex Synaptosomes Challenged with Amyloid β-Peptide

“…The gradient elution was achieved by a solution of water‐acetonitrile (93:7 ratio, with 3% of acetic acid) as initial conditions. The complete separation was achieved in 60 min by means of a validated method (Locatelli et al , ) and herein applied after evaluation of analytical performances and the absence of matrix interferences specifically for the harpagoside quantification.…”

Identification of phenolic components via LC–MS analysis and biological activities of two Centaurea species: C. drabifolia subsp. drabifolia and C. lycopifolia

“…This fact has been growing day by day and the last century has witnessed a growing number of herbal consumers and could be considered as "herbal products era" against synthetics. From this point, global awareness on natural products have resulted in a plethora of investigations focused towards the discovery of novel and effective compounds from plants [1,2]. One famous example is the discovery of the antimalarial drugs, Artemisinin isolated from the traditionally used medicinal plant, Artemisia annua, which was awarded the 2015 Nobel Prize in Physiology or Medicine [3].…”

HPLC–PDA Polyphenolic Quantification, UHPLC–MS Secondary Metabolite Composition, and In Vitro Enzyme Inhibition Potential of Bougainvillea glabra

“…After solubilization, the samples were centrifuged at 12,000× g and filtered through a micro-filter before HPLC injection. Standard phenolic compounds were applied for identifying and quantifying individual phenolic components in the samples as µg/g dry extract [21,22].…”