Protective Effects Induced by Microwave-Assisted Aqueous Harpagophytum Extract on Rat Cortex Synaptosomes Challenged with Amyloid β-Peptide

Abstract

Harpagophytum procumbens is a plant species that displays anti-inflammatory properties in multiple tissues. The iridoid glycosides arpagoside, harpagide, and procumbide appear to be the most therapeutically important constituents. In addition, harpagoside treatment exerted neuroprotective effects both in vitro and in vivo. Considering these findings, the aim of the present work is to explore the possible protective role of the previously described microwave-assisted aqueous extract of H. procumbens on rat hypothalamic (Hypo-E22) cells, and in rat cortex challenged with amyloid β-peptide (1-40). In this context, we assayed the protective effects induced by H. procumbens by measuring the levels of malondialdehyde, 3-hydroxykynurenine (3-HK), brain-derived neurotrophic factor, and tumor necrosis factor-α, 3-HK. Finally, we evaluated the effects of H. procumbens treatment on cortex levels of dopamine, norepinephrine, and serotonin. H. procumbens extract was well tolerated by Hypo-E22 cells and upregulated brain-derived neurotrophic factor gene expression but down-regulated tumor necrosis factor-α gene expression. In addition, the extract reduced amyloid β-peptide stimulation of malondialdehyde and 3-HK and blunted the decrease of dopamine, norepinephrine, and serotonin, in the cortex. In this context, our work supports further studies for the evaluation and confirmation of Harpagophytum in the management of the clinical symptoms related to Alzheimer's disease. Copyright © 2017 John Wiley & Sons, Ltd.

Protective effects induced by alcoholic Phlomis fruticosa and Phlomis herba‐venti extracts in isolated rat colon: Focus on antioxidant, anti‐inflammatory, and antimicrobial activities in vitro

“…Consistent with these antioxidant effects, we observed that alcoholic P. fruticosa and P. herba‐venti extracts displayed a significant reduction of LPS‐induced increase in MDA levels (Figure ), a recognized biomarker of lipid peroxidation and tissue damage (Ferrante et al, ; Mancuso et al, ). Alcoholic P. fruticosa and P. herba‐venti extracts were as effective as sulfasalazine in restoring basal MDA levels in isolated rat colon challenged with LPS.…”

Devil's claw (Harpagophytum procumbens) and chronic inflammatory diseases: A concise overview on preclinical and clinical data

“…Consistently, it has been recently observed that harpagoside could The extract was able to decrease the burden of oxidative stress and to protect from the decline in cortical dopamine (DA), norepinephrine (NE) and serotonin (5-hydroxytriptamine, 5-HT) levels induced by amyloid β-peptide Ferrante et al, 2017 inhibit interleukin (IL)-6 production from primary human osteoarthritis chondrocytes challenged with IL-1β (Haseeb, Ansari, & Haqqi, 2017).…”

“…More recently, we have evaluated the protective effects of an aqueous extract of devil's claw roots (1.5% harpagoside) in an experimental model of Alzheimer Disease, in vitro . This extract was able to decrease the burden of oxidative stress and protect from the decline in cortical dopamine (DA), norepinephrine (NE), and serotonin (5‐hydroxytriptamine, 5‐HT) levels induced by amyloid β‐peptide, in rat cortex synaptosomes (Ferrante et al, ). Intriguingly, the multitarget mechanisms showed by both devil's claw extracts and harpagoside corroborate recent in vitro studies regarding the neuroprotective effects induced by iridoid compounds such as loganin and genipin (Liu, Chen, Lu, Fang, & Yu, ; Tseng, Lin, Chang, & Lo, ), thus supporting future in vivo investigations in order to confirm the efficacy and safety in the management of neurodegenerative diseases.…”

Biopotential of Bersama abyssinica Fresen Stem Bark Extracts: UHPLC Profiles, Antioxidant, Enzyme Inhibitory, and Antiproliferative Propensities

“…Kynurenic acid was reportedly produced in multiple tissues, including brain and peripheral organs [26], although pharmacokinetic studies excluded any possibility of the peripheral pool crossing the blood-brain barrier [27]. In the brain, the kynurenine-derived kynurenic acid was described as a reliable marker of neuroprotection [28,29], whereas it seemed to be involved in an inflammatory response at the peripheral level [30]. Kynurenic acid was also described as an antiproliferative factor toward colon, renal, and glioma cells [31].…”