Multicolorimetric ELISA biosensors on a paper/polymer hybrid analytical device for visual point-of-care detection of infection diseases

A simple and rapid strategy for monitoring vancomycin concentration in serum using a multicolor immunosensor based on the ratio of gold nanobipyramids and a product of cetyltrimethylammonium bromide-blue oxide of 3,3′,5,5′-tetramethylbenzidine interaction

“…Initially, we investigated the effect of HCl concentration on the multicolor system according to the traditional strategies (HCl acts as a secondary proton donor to induce the oxidation of TMB + to TMB 2+ , and TMB 2+ further efficiently etches AuNBPs). 17,31 As shown in Fig. S2, † the longitudinal LSPR peaks of AuNBPs blue-shied when the HCl concentration was higher 0.25 M. However, the reaction systems have more vivid colors in 0-0.1 M HCl (Fig.…”

Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review

“…In this study, a synthesized platinum-decorated gold nanoparticle (Au@Pt NP) that displayed high catalytic activity was utilized for naked-eye detection less than 40 min, and the LOD was 0.1 pg/mL of N protein in a throat swab sample. Ma et al reported an indirect multicolorimetric ELISA integrated with a microfluidic device for rapid visual detection of the hepatitis C virus, with an LOD of 9.1 ng/µL within 50 min [ 52 ]. Kasetsirikul et al reported ELISA-based colorimetric detection of SARS-CoV-2 antibody, and the LOD was 9 ng/mL [ 53 ].…”

A microfluidic fully paper-based analytical device integrated with loop-mediated isothermal amplification and nano-biosensors for rapid, sensitive, and specific quantitative detection of infectious diseases

“…Microfluidic lab-on-a-chip (LOC) offers a unique opportunity for highly efficient human health diagnostics 22–29 because of various advantages, including miniaturization, integration, automation, and low sample consumption. The LOC technology provides high potential for the point of care (POC) diagnosis of a wide range of diseases in low-resource settings.…”

“…[18][19][20] However, like qPCR, specialized equipment and laborious procedure are required for conventional LAMP, which limits its broad application in the field or the low-resource settings. 21 Microfluidic lab-on-a-chip (LOC) offers a unique opportunity for highly efficient human health diagnostics [22][23][24][25][26][27][28][29] because of various advantages, including miniaturization, integration, automation, and low sample consumption. The LOC technology provides high potential for the point of care (POC) diagnosis of a wide range of diseases in low-resource settings.…”