A microfluidic fully paper-based analytical device integrated with loop-mediated isothermal amplification and nano-biosensors for rapid, sensitive, and specific quantitative detection of infectious diseases

Abstract

Bacterial meningitis, an infection of the membranes (meninges) and cerebrospinal fluid (CSF) surrounding the brain and spinal cord, is one of the major causes of death and disability worldwide. Higher...

A three-in-one microfluidic droplet digital PCR platform for absolute quantitative analysis of DNA

“…† Overall, our platform offers superior performance in terms of high-level integration, extended detection range, reduced detection time and lower cost. Furthermore, compared to existing integrated ddPCR platforms 7,9,13 and digital isothermal amplification platforms, [25][26][27] our three-in-one ddPCR platform shows high potential in laboratory-to-product translation due to the easily mass-produced PC material and the matching control instrument. With an increasing demand for sensitive and accurate nucleic acid quantification in tumor diagnosis, pathogen detection, and prenatal diagnosis, our platform shows promising market application prospects.…”

Hydraulic–electric analogy for design and operation of microfluidic systems

“…With these improvements, the microfluidic circuits can accelerate the development of new diagnostic and therapeutic tools and find numerous applications in point-of-care diagnosis, drug delivery, and other biochemical applications. 77,126,127 Author contributions…”

Advances in miniaturized nanosensing platforms for analysis of pathogenic bacteria and viruses

“…The smartphone detection of the emitted light showed the high sensitivity of the developed sensor that enabled analysis of E. coli O157:H7 with a detection limit of 4.0 CFU mL −1 . Such sensors and other developed smart sensors [50][51][52][53][54][55][56] proved the ability to miniaturize analytical biodevices to be employed for POC testing of various pathogens for early diagnosis of infectious diseases. Different medical companies have already started commercialization of such nanobiodevices and developed them to be used all over the world, especially in case of a pandemic.…”