In the present study, we introduce
a new approach for rapid bonding
of poly(methyl methacrylate) (PMMA)-based microdevices using an acetic
acid solvent with the assistance of UV irradiation. For the anticipated
mechanism, acetic acid and UV irradiation induced free radicals on
the PMMA surfaces, and acrylate monomers subsequently formed cross-links
to create a permanent bonding between the PMMA substrates. PMMA devices
effectively bonded within 30 s at a low pressure using clamps, and
a clogging-free microchannel was achieved with the optimized 50% acetic
acid. For surface characterizations, contact angle measurements and
bonding performance analyses were conducted using predetermined acetic
acid concentrations to optimize bonding conditions. In addition, the
highest bond strength of bonded PMMA was approximately 11.75 MPa,
which has not been reported before in the bonding of PMMA. A leak
test was performed over 180 h to assess the robustness of the proposed
method. Moreover, to promote the applicability of this bonding method,
we tested two kinds of microfluidic device applications, including
a cell culture-based device and a metal microelectrode-integrated
device. The results showed that the cell culture-based application
was highly biocompatible with the PMMA microdevices fabricated using
an acetic acid solvent. Moreover, the low pressure required during
the bonding process supported the integration of metal microelectrodes
with the PMMA microdevice without any damage to the metal films. This
novel bonding method holds great potential in the ecofriendly and
rapid fabrication of microfluidic devices using PMMA.
We introduce a new strategy for fabricating a seamless three-dimensional (3D) helical microreactor utilizing a silicone tube and a paraffin mold. With this method, various shapes and sizes of 3D helical microreactors were fabricated, and a complicated and laborious photolithographic process, or 3D printing, was eliminated. With dramatically enhanced portability at a significantly reduced fabrication cost, such a device can be considered to be the simplest microreactor, developed to date, for performing the flow-through polymerase chain reaction (PCR).
The development of improved methods for the synthesis of monodisperse gold nanoparticles (Au NPs) is of high priority because they can be used as substrates for surface-enhanced Raman scattering (SERS) applications relating to biological lipids.
Pathogen detection by nucleic acid amplification proved its significance during the current coronavirus disease 2019 (COVID-19) pandemic. The emergence of recombinase polymerase amplification (RPA) has enabled nucleic acid amplification in limited-resource conditions owing to the low operating temperatures around the human body. In this study, we fabricated a wearable RPA microdevice using poly(dimethylsiloxane) (PDMS), which can form soft—but tight—contact with human skin without external support during the body-heat-based reaction process. In particular, the curing agent ratio of PDMS was tuned to improve the flexibility and adhesion of the device for better contact with human skin, as well as to temporally bond the microdevice without requiring further surface modification steps. For PDMS characterization, water contact angle measurements and tests for flexibility, stretchability, bond strength, comfortability, and bendability were conducted to confirm the surface properties of the different mixing ratios of PDMS. By using human body heat, the wearable RPA microdevices were successfully applied to amplify 210 bp from Escherichia coli O157:H7 (E. coli O157:H7) and 203 bp from the DNA plasmid SARS-CoV-2 within 23 min. The limit of detection (LOD) was approximately 500 pg/reaction for genomic DNA template (E. coli O157:H7), and 600 fg/reaction for plasmid DNA template (SARS-CoV-2), based on gel electrophoresis. The wearable RPA microdevice could have a high impact on DNA amplification in instrument-free and resource-limited settings.
We demonstrate the enhancement of fluorescence emission from a dye, 5-carboxyfluorescein (FAM), which couples with surface plasmons at the spectral channels of excitation and emission. Experiments and calculations revealed that bimetallic (gold-silver) plasmon, as compared to the monometallic ones, allowed such coupling to be enhanced, at both the spectral channels. We achieved the maximum fluorescence enhancement level of 46.5-fold, with markedly high reproducibility (coefficient of variation ~ 0.5%) at a FAM concentration of 10 nM. We also found that higher fluorescence enhancement was more likely to be reproducible. This encourages the use of this technology for practical applications in fluorescence-based biochemical assays. Moreover, we investigated a FAM concentration-dependent enhancement of fluorescence. It was found that fluorescence enhancement decreased and saturated at above 10 nM concentration possibly due to partial photo-bleaching of FAM molecules.
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