Multicolor diagnosis of salivary alkaline phosphatase triggered by silver-coated gold nanobipyramids

A multicolor biosensor for alkaline phosphatase activity detection based on the peroxidase activity of copper nanoclusters and etching of gold nanorods

“…32 Hafez et al reported a multicolor biosensor for ALP activity detection based on the simultaneous modification of both the composition and morphology of AuNBPs. 33 The detection ranges of these methods are larger, but the variety of colors is less. On the contrary, our proposed method is rich in color and has higher color resolution, which is more suitable for naked-eye detection (Table 1).…”

“…Some multicolor biosensors for ALP detection have been reported. [31][32][33] For example, Zhang et al developed a multicolor biosensor for ALP detection based on iodine-mediated etching of AuNRs since different aspect ratios of AuNRs display different colors. 31 The detection limit of this method is low, but the color response is not sensitive in the detection range, so the naked-eye detection sensitivity is low.…”

Detection of Periodontal Disease Marker with Geometrical Transformation of Ag Nanoplates

“…Saliva samples were directly collected from the mouth of different volunteers aged 30–35 years, via a plastic catheter after 3 min of mouth cleansing before breakfast. Then, the saliva was collected in sterile test tubes, before centrifugation at 1000 rpm/15 min and storing at −80 °C till both ALP and IL-1β analysis. , For ALP and IL-1β analysis, the collected saliva was diluted 10 times in 20 mM Tris base solution, then they were assayed following the same procedures described for both ALP and IL-1β analysis considering the standard addition method for determining the unknown ALP and IL-1β in saliva using standard ALP and IL-1β solutions to get the concentration of unknown ALP and IL-1β in saliva. The recovery rate for our developed plasmonic sensor was calculated according to the following equation: normalr normale normalc normalo normalv normale normalr normaly .25em normalr normala normalt normale ( % ) = normalm normale normala normals normalu normalr normale normald .25em normalc normalo normaln normalc normale normaln normalt normalr normala normalt normali normalo normaln normala normald normald normale normald .25em normalc normalo normaln normalc normale normaln normalt normalr normala normalt normali normalo normaln × 100 …”

“…Saliva samples were directly collected from the mouth of different volunteers aged 30–35 years, via a plastic catheter after 3 min of mouth cleansing before breakfast. Then, the saliva was collected in sterile test tubes, before centrifugation at 1000 rpm/15 min and storing at −80 °C till both ALP and IL-1β analysis. , For ALP and IL-1β analysis, the collected saliva was diluted 10 times in 20 mM Tris base solution, then they were assayed following the same procedures described for both ALP and IL-1β analysis considering the standard addition method for determining the unknown ALP and IL-1β in saliva using standard ALP and IL-1β solutions to get the concentration of unknown ALP and IL-1β in saliva. The recovery rate for our developed plasmonic sensor was calculated according to the following equation: …”

Rapid and sensitive determination of ascorbic acid based on label-free silver triangular nanoplates

“…As a matter of fact, there are two necessary conditions for establishing a multi-color colorimetric sensor that can be distinguished by the naked eye: First, the sensor should display rich color changes so that they can be easily distinguished visually; Second, there should be a one-to-one relationship between the concentration of the analyte and the corresponding color( Ma et al, 2016 ). In recent years, polychromatic sensors based on gold nanorods, gold nano bipyramids, and gold nanospheres have been widely praised by researchers, while the importance of precious metal silver nanomaterials in polychromatic detection has been ignored( Hafez et al, 2021 ; Y. Liu et al, 2018 ; Y.…”