Multicenter evaluation of the Abbott IMx CA 15-3 assay

Kamp, Gerard J. van; Bon, G.G.; Verstraeten, Rob A.; Lynch, Don M.; Krikau, M; Fluckiger, Jacob U.; Ruibal, A; Kenemans, P.

doi:10.1093/clinchem/42.1.28

Cited by 14 publications

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“…Thus, CA15-3 is one of the important biomarkers commonly used in monitoring therapy outcomes and disease progression in metastatic breast cancer patients . CA15-3 has been detected with commercialized enzyme-linked immunosorbent assay (ELISA), radioimmunometric assay (RIA), and microparticle enzyme immunoassay (MEIA) . These sandwich-based immunoassays indirectly detect CA15-3 with enzyme or radioisotopically labeled secondary antibodies.…”

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confidence: 99%

Rapid and Label-Free Detection of Breast Cancer Biomarker CA15-3 in Clinical Human Serum Samples with Optofluidic Ring Resonator Sensors

et al. 2009

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Sensitive and specific detection of breast cancer biomarker CA15-3 in human serum is an important step toward successful evaluation of clinical treatment and prediction of breast cancer recurrence. In this work, we developed an optofluidic ring resonator (OFRR) sensor and the corresponding sensing protocols for label-free CA15-3 detection without any additional signal amplification steps. Nonspecific serum protein adsorption was minimized with effective surface blocking methods. The sensor performance for CA15-3 detection was first characterized in phosphate-buffered saline (PBS) buffer and in fetal calf serum. Then the potential use of the OFRR as a simple clinical laboratory testing device for breast cancer diagnostics was tested by measuring the CA15-3 level in clinical human serum samples, and the results were compared with those of standard clinical lab tests. It was found that the OFRR was capable of detecting approximately 1 unit/mL CA15-3 in both PBS buffer and diluted serum within approximately 30 min. Our work marks the first demonstration of the optical ring resonator biosensor in real clinical applications that features low cost, simple detection procedures, rapid response time, low sample consumption, and high specificity.

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confidence: 99%

Rapid and Label-Free Detection of Breast Cancer Biomarker CA15-3 in Clinical Human Serum Samples with Optofluidic Ring Resonator Sensors

et al. 2009

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Sensitive detection of tumor marker CA15‐3 in human serum by capillary electrophoretic immunoassay with chemiluminescence detection

Liu¹,

Zheng

Cao

et al. 2008

J of Separation Science

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A new and sensitive non-competitive immunoassay (IA) for tumor marker carbohydrate antigen 15-3 (CA15-3) by CE coupling with ECL detection has been developed. This method is based on luminol-H(2)O(2 )reaction catalyzed by horseradish peroxidase (HRP). The optimum CE separation and CL detection conditions were investigated. After the non-competitive immunoreaction, the free HRP-labeled CA15-3 antibody (Ab*) and the bound Ab*-antigen (Ab*-Ag) complex were separated in a separation capillary and then catalyzed the CL reaction of luminol and H(2)O(2 )in a reaction capillary following the separation capillary. The calibration curve based on the peak areas of Ab*-Ag complex plotted against the concentrations of CA15-3 is in the range of 0-250 U/mL with a correlation coefficient of 0.9983 and the detection limit is 0.035 U/mL (S/N = 3). The response for five consecutive injections of 125 U/mL CA15-3 resulted in RSDs of 0.83% and 3.1% for the migration time and the peak area, respectively. The method was successfully used for the quantification of CA15-3 in human sera obtained from healthy persons and from patients with breast cancer.

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Estimation of Probability of Malignancy Using a Logistic Model Combining Physical Examination, Ultrasound, Serum CA 125, and Serum CA 72-4 in Postmenopausal Women with a Pelvic Mass: An International Multicenter Study

Schutter¹,

Sohn

Kristen

et al. 1998

Gynecologic Oncology

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Multicenter evaluation of the Abbott IMx CA 15-3 assay

Cited by 14 publications

References 0 publications

Rapid and Label-Free Detection of Breast Cancer Biomarker CA15-3 in Clinical Human Serum Samples with Optofluidic Ring Resonator Sensors

Rapid and Label-Free Detection of Breast Cancer Biomarker CA15-3 in Clinical Human Serum Samples with Optofluidic Ring Resonator Sensors

Sensitive detection of tumor marker CA15‐3 in human serum by capillary electrophoretic immunoassay with chemiluminescence detection

Estimation of Probability of Malignancy Using a Logistic Model Combining Physical Examination, Ultrasound, Serum CA 125, and Serum CA 72-4 in Postmenopausal Women with a Pelvic Mass: An International Multicenter Study

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