Multi-target electrochemical malaria aptasensor on flexible multielectrode arrays for detection in malaria parasite blood samples

DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection

“…The electrochemical detection of the S protein was made utilizing well-established flexible multi-electrode arrays (flex-MEAs) [ 52 ]. Briefly, the flex-MEA chip was incubated for 2 h in a 0.5 μM C7 aptamer solution.…”

Voltammetric Responses of a CYP2D6‐Based Biosensor to 3,4‐methylenedioxymethamphetamine (MDMA) and the Synthetic Cathinone α‐pyrrolidinopentiophenone (α‐PVP).

“…Moreover, considering that the cathodic peak generated for both species occur at different E p (−298 mV for α‐PVP and −321 mV for MDMA), it is observed that the electrochemical response in the α‐PVP + MDMA matrix shifts to E p values closer to that characteristic of the electrochemical response of MDMA than to those of α‐PVP, which is why this matrix does not favor the detection of α‐PVP. In this regard, one of the strategies that could eliminate this overlapping of cathode peaks and thus reduce relative error would be the implementation of a sample pretreatment step based on chromatographic separation, or a novel alternative, which is the use of a multi‐electrode system, in this case a multisensor system, where a simultaneous detection of the different components can be performed after a separation phase of matrix components [38,39] …”

Electrochemical Aptamer-Based Sensors with Tunable Detection Range

“…Additionally, for aptamers with cross-selectivity for more than one target, the different signals from the individual electrodes with other aptamers could help to distinguish the targets by logic gate operation . For instance, Mayer et al employed a multielectrode array with four kinds of aptamers to realize the detection of two common malaria parasites (Plasmodium falciparum and Plasmodium vivax) . For both Plasmodium falciparum and Plasmodium vivax , plasmodium lactate dehydrogenase is expressed and named as Plasmodium falciparum lactate dehydrogenase (PfLDH) and Plasmodium vivax lactate dehydrogenase (PvLDH), respectively.…”

“…125 For instance, Mayer et al employed a multielectrode array with four kinds of aptamers to realize the detection of two common malaria parasites (Plasmodium falciparum and Plasmodium vivax). 126 For both Plasmodium falciparum and Plasmodium vivax, plasmodium lactate dehydrogenase is expressed and named as Plasmodium falciparum lactate dehydrogenase (PfLDH) and Plasmodium vivax lactate dehydrogenase (PvLDH), respectively. However, histidine-rich protein 2 (HRP-2) is exclusively expressed by Plasmodium falciparum.…”