Electrochemical Aptamer-Based Sensors with Tunable Detection Range

Pan, Jing; Xu, Wenxia; Li, Wanlu; Chen, Shuwen; Dai, Yu; Yu, Shanwu; Zhou, Qitao; Xia, Fan

doi:10.1021/acs.analchem.2c04498

Cited by 23 publications

(11 citation statements)

References 161 publications

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“…Under the same experimental conditions, the histogram in Figure shows that the intensities of the ratiometric signals of the nonspecific targets were close to that of the blank control and were significantly lower than that of the target. The high selectivity can be ascribed to the aptamer-based specific discrimination. , …”

Section: Resultsmentioning

confidence: 99%

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Amplification-Free Ratiometric Electrochemical Aptasensor for Point-of-Care Detection of Therapeutic Monoclonal Antibodies

Wan,

Liang,

et al. 2023

Anal. Chem.

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The rapid quantification of therapeutic monoclonal antibodies (mAbs) is of great significance to their pharmacokinetics/pharmacodynamics (PK/PD) research and the personalized medication for disease treatment. Taking advantage of the direct decoration of tens of redox tags to the target of interest, we illustrate herein an amplification-free ratiometric electrochemical aptasensor for the point-of-care (POC) detection of trace amounts of therapeutic mAbs. The POC detection of therapeutic mAbs involved the use of the methylene blue (MB)-conjugated aptamer as the affinity element and the decoration of therapeutic mAbs with ferrocene (Fc) tags via the boronate crosslinking, in which the MB-derived peak current was used as the reference signal, and the peak current of the Fc tag was used as the output signal. As each therapeutic mAb carries tens of diol sites for the site-specific decoration of the Fc output tags, the boronate crosslinking enabled the amplification-free detection, which is cost-effective and quite simple in operation. In the presence of bevacizumab (BevMab) as the target, the resulting ratiometric signal (i.e., the I Fc/I MB value) exhibited a good linear response over the range of 0.025–2.5 μg/mL, and the limit of detection (LOD) of the electrochemical aptasensor was 6.5 ng/mL. Results indicated that the aptamer-based affinity recognition endowed the detection of therapeutic mAbs with high selectivity, while the ratiometric readout exhibited satisfactory reproducibility and robustness. Moreover, the ratiometric electrochemical aptasensor is applicable to the detection of therapeutic mAbs in serum samples. Taking together, the amplification-free ratiometric electrochemical aptasensor holds great promise in the POC detection of therapeutic mAbs.

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Section: Resultsmentioning

confidence: 99%

“…The high selectivity can be ascribed to the aptamer-based specific discrimination. 51,52 Detection of Therapeutic mAbs in Serum Samples. To investigate the reliability of the electrochemical aptasensor toward target detection in biological samples, human serum was selected as the matrix.…”

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confidence: 99%

Amplification-Free Ratiometric Electrochemical Aptasensor for Point-of-Care Detection of Therapeutic Monoclonal Antibodies

Wan,

Liang,

et al. 2023

Anal. Chem.

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“…[31][32][33][34] Particularly, aptamer-based biosensors usually generate signals via structural reconfigurations of the aptamers induced by target binding, which also enables "reagent-free" detection. [33][34][35] Recently, nanopores decorated with aptamers have also enabled direct detection of SARS-CoV-2 viral particles with concentrations down to 1 × 10 4 copies per ml. 28 However, there are some common challenges in the application of aptamer-based bioassays.…”

Section: Aptamer-based Antigen Sensorsmentioning

confidence: 99%

Recent development of nanotechnology-empowered antigen assay methods for the control of infectious diseases

Peng

Fan

et al. 2023

Analyst

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“…The precise identification of miRNA is crucial for the prevention, treatment, and prediction of cancer. Although numerous detection methods have been developed, most rely on biological recognition mechanisms and have a fixed sensitivity and dynamic range that limit their effectiveness. − It should be noted that the concentration of nucleic acid varies between different samples, making it difficult to detect different nucleic acids within a fixed dynamic range. − Thus, it is imperative to develop a highly adjustable and sensitive dynamic range for miRNA analysis. Various natural approaches have recently been utilized to finely adjust the dynamic range of biosensors. − For example, Porchetta and co-workers demonstrated two methods, namely, distal-site mutation and allosteric inhibition, that change the switching equilibrium constant to adjust the detection performance of aptasensors over several orders of magnitude .…”

Section: Introductionmentioning

confidence: 99%

DNA Tile and Invading Stacking Primer-Assisted CRISPR–Cas12a Multiple Amplification System for Entropy-Driven Electrochemical Detection of MicroRNA with Tunable Sensitivity

Wang,

Li,

Fan

et al. 2023

Anal. Chem.

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Conventional electrochemical detection of microRNA (miRNA) encounters issues of poor sensitivity and fixed dynamic range. Here, we report a DNA tile and invading stacking primer-assisted CRISPR− Cas12a multiple amplification strategy to construct an entropy-controlled electrochemical biosensor for the detection of miRNA with tunable sensitivity and dynamic range. To amplify the signal, a cascade amplification of the CRISPR−Cas12a system along with invading stacking primer signal amplification (ISPSA) was designed to detect trace amounts of miRNA-31 (miR-31). The target miR-31 could activate ISPSA and produce numerous DNAs, triggering the cleavage of the single-stranded linker probe (LP) that connects a methylene blue-labeled DNA tile with a DNA tetrahedron to form a Y-shaped DNA scaffold on the electrode. Based on the decrease of current, miR-31 can be accurately and efficiently detected. Impressively, by changing the loop length of the LP, it is possible to finely tune the entropic contribution while keeping the enthalpic contribution constant. This strategy has shown a tunable limit of detection for miRNA from 0.31 fM to 0.56 pM, as well as a dynamic range from ∼2200fold to ∼270,000-fold. Moreover, it demonstrated satisfactory results in identifying cancer cells with a high expression of miR-31. Our strategy broadens the application of conventional electrochemical biosensing and provides a tunable strategy for detecting miRNAs at varying concentrations.

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Electrochemical Aptamer-Based Sensors with Tunable Detection Range

Cited by 23 publications

References 161 publications

Amplification-Free Ratiometric Electrochemical Aptasensor for Point-of-Care Detection of Therapeutic Monoclonal Antibodies

Amplification-Free Ratiometric Electrochemical Aptasensor for Point-of-Care Detection of Therapeutic Monoclonal Antibodies

Recent development of nanotechnology-empowered antigen assay methods for the control of infectious diseases

DNA Tile and Invading Stacking Primer-Assisted CRISPR–Cas12a Multiple Amplification System for Entropy-Driven Electrochemical Detection of MicroRNA with Tunable Sensitivity

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