DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection

Martínez-Roque, Mateo Alejandro; Franco-Urquijo, Pablo Alberto; García-Velásquez, Victor Miguel; Choukeife, Moujab; Mayer, Guenter; Molina-Ramírez, Sergio Roberto; Figueroa-Miranda, Gabriela; Mayer, Dirk; Álvarez-Salas, Luis Marat

doi:10.1016/j.ab.2022.114633

Cited by 15 publications

(11 citation statements)

References 74 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the onset of the COVID‐19 pandemic, many aptamers that recognize protein biomarkers from SARS‐CoV‐2 have been reported, [19–40] most of which target the spike protein (S‐protein) of the virus for binding largely because the S‐protein is on the surface of viral particles (and thus is easily accessible) and plays essential roles in virus fusion into host cells [41,42] . These aptamers were isolated using different selection methods (beads‐, filtration‐ or electrophoresis‐based SELEX) and various forms of S‐proteins as binding targets (receptor‐binding domain (RBD), subunit S1, trimeric S‐protein, pseudovirus with S‐protein expressed) [43] .…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Diverse DNA Aptamers for Recognition of Spike Proteins of Multiple SARS‐CoV‐2 Variants

Zhang

Amini

et al. 2023

Analysis & Sensing

View full text Add to dashboard Cite

Many DNA aptamers have been reported to target the spike protein (S‐protein) of SARS‐CoV‐2. These aptamers display different affinities or recognize different epitopes of the S‐protein. We conducted a comparative study of 9 DNA aptamers for binding to several variants of the S‐protein and pseudoviruses using the same testing methods, including dot‐blot assays and enzyme‐linked aptamer binding assays, to evaluate their affinity ranking and analytical utility. Moreover, the binding sites of these aptamers on the S‐protein were examined using aptamer competition assays to understand the effect of S‐protein mutations on aptamer affinity and the degree of overlapping of the binding sites by these aptamers.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Diverse DNA Aptamers for Recognition of Spike Proteins of Multiple SARS‐CoV‐2 Variants

Zhang

Amini

et al. 2023

Analysis & Sensing

View full text Add to dashboard Cite

show abstract

“…Today, research groups have developed different electrode set ups and detection schemes, as well as expanded the menu of analytes, which now includes small molecule drugs (cocaine, 104 ampicilin 105 ), proteins (CRP, 106 insulin 107 ), and viruses (zika virus, 108 avian flu, 109 SARS-CoV-2 (ref. 59 and 110–116)).…”

Section: In Vitro Applicationsmentioning

confidence: 97%

Aptamers 101: aptamer discovery andin vitroapplications in biosensors and separations

et al. 2023

View full text Add to dashboard Cite

show abstract

“…efficiency 85%-89%). In order to develop a highthroughput detection method for SARS-CoV-2, two noncompeting aptamers (C7 and C9) against the S protein were isolated by ideal-filter capillary electrophoresis SELEX by Alvarez-Salas et al [32] (Table 1). As the S protein recognition elements, C7 and C9 were used to develop electrochemical sensors and a fluorophore-linked aptamer assay test for SARS-CoV-2.…”

Section: Cov-2 S Proteinmentioning

confidence: 99%

“…Moreover, A1C1 inhibited the interaction between S protein and ACE2 (blocking efficiency 85%–89%). In order to develop a high‐throughput detection method for SARS‐CoV‐2, two noncompeting aptamers (C7 and C9) against the S protein were isolated by ideal‐filter capillary electrophoresis SELEX by Alvarez‐Salas et al [32] . (Table 1).…”

Section: Aptamers Developed For Sars‐cov‐2mentioning

confidence: 99%

Aptamer‐based strategies against SARS‐CoV‐2 viruses

Huang

Chen

Zhang

et al. 2023

BMEMat

View full text Add to dashboard Cite

The COVID‐19 pandemic, caused by SARS‐CoV‐2, has seriously threatened human life and security in recent years and has had a serious impact on economic development. A range of strategies, including antibodies, small‐molecule agents and vaccines, have been developed and widely used to combat SARS‐CoV‐2. However, the uncertain development and susceptibility to mutation of SARS‐CoV‐2 pose challenges to these approaches, making it necessary to develop a broader range of strategies to complement and expand the diversity of attacks against SARS‐CoV‐2. As promising tools against SARS‐CoV‐2, aptamers have attracted wide attention due to their unique molecular properties. In this review, we survey SARS‐CoV‐2 aptamers and aptamer‐based detection tools as well as aptamer‐based therapeutics. In addition, we analyze the potential value of aptamers in the investigation of SARS‐CoV‐2 infection mechanism and vaccine design. Finally, we look forward to the future development direction of aptamers against SARS‐CoV‐2 or other viruses.

show abstract

DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection

Cited by 15 publications

References 74 publications

Comparative Characterization of Diverse DNA Aptamers for Recognition of Spike Proteins of Multiple SARS‐CoV‐2 Variants

Comparative Characterization of Diverse DNA Aptamers for Recognition of Spike Proteins of Multiple SARS‐CoV‐2 Variants

Aptamers 101: aptamer discovery andin vitroapplications in biosensors and separations

Aptamer‐based strategies against SARS‐CoV‐2 viruses

Contact Info

Product

Resources

About