Aptamer‐based strategies against SARS‐CoV‐2 viruses

Huang, Yihao; Chen, Ximing; Zhang, Jialü; Tian, Wenhai; Liu, Siwen; Tam, Rachel; Yang, Chaoyong; Song, Yanling

doi:10.1002/bmm2.12024

Cited by 6 publications

(4 citation statements)

References 87 publications

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“…An electrochemical sensor detects chemical changes around the device and converts the signal into electric current or electrical resistance on the electrode surface. Electrochemical sensors can be used repeatedly with high sensitivity [ 112 , 119 , 120 ]. For example, Idili et al developed an electrochemical aptamer-based (EAB) sensor [ 101 ] for the SARS-CoV-2 S protein to detect changes in the current occurring under aptamer-target binding.…”

Section: Aptamersmentioning

confidence: 99%

Aptamers and Nanobodies as New Bioprobes for SARS-CoV-2 Diagnostic and Therapeutic System Applications

Park,

Lee

et al. 2024

Biosensors

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The global challenges posed by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic have underscored the critical importance of innovative and efficient control systems for addressing future pandemics. The most effective way to control the pandemic is to rapidly suppress the spread of the virus through early detection using a rapid, accurate, and easy-to-use diagnostic platform. In biosensors that use bioprobes, the binding affinity of molecular recognition elements (MREs) is the primary factor determining the dynamic range of the sensing platform. Furthermore, the sensitivity relies mainly on bioprobe quality with sufficient functionality. This comprehensive review investigates aptamers and nanobodies recently developed as advanced MREs for SARS-CoV-2 diagnostic and therapeutic applications. These bioprobes might be integrated into organic bioelectronic materials and devices, with promising enhanced sensitivity and specificity. This review offers valuable insights into advancing biosensing technologies for infectious disease diagnosis and treatment using aptamers and nanobodies as new bioprobes.

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Section: Aptamersmentioning

confidence: 99%

Aptamers and Nanobodies as New Bioprobes for SARS-CoV-2 Diagnostic and Therapeutic System Applications

Park,

Lee

et al. 2024

Biosensors

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“…Researchers are working on developing biosensors that can detect and analyze subtle conditions in individuals using advanced biosensing materials. − Similarly, DNA has become a fascinating material for biosensor engineering due to its essential role in organisms. , A biosensor typically involves a target detection component and a transducer that produces enhanced signals when interacting with target analytes. Developing biosensors that can detect target analytes with a low concentration is a major concern .…”

Section: Introductionmentioning

confidence: 99%

DNA and Nanomaterials: A Functional Combination for DNA Sensing

Quazi,

Choi,

Kim

et al. 2024

ACS Appl. Bio Mater.

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Recent decades have experienced tough situations due to the lack of reliable diagnostic facilities. The most recent cases occurred during the pandemic, where researchers observed the lack of diagnostic facilities with precision. Microorganisms and viral disease's ability to escape diagnosis has been a global challenge. DNA always has been a unique moiety with a strong and precise base-paired structure. DNA in human and foreign particles makes identification possible through base pairing. Since then, researchers have focused heavily on designing diagnostic assays targeting DNA in particular. Moreover, DNA nanotechnology has contributed vastly to designing composite nanomaterials by combining DNA/nucleic acids with functional nanomaterials and inorganic nanoparticles exploiting their physicochemical properties. These nanomaterials often exhibit unique or enhanced properties due to the synergistic activity of the many components. The capabilities of DNA and additional nanomaterials have shown the combination of robust and advanced tailoring of biosensors. Preceding findings state that the conventional strategies have exhibited certain limitations such as a low range of target detection, less biodegradability, subordinate half-life, and high susceptibility to microenvironments; however, a DNA−nanomaterial-based biosensor has overcome these limitations meaningfully. Additionally, the unique properties of nucleic acids have been studied extensively due to their high signal conduction abilities. Here, we review recent studies on DNA−nanomaterial-based biosensors, their mechanism of action, and improved/updated strategies in vivo and in situ. Furthermore, this review highlights the recent methodologies on DNA utilization to exploit the interfacial properties of nanomaterials in DNA sensing. Lastly, the review concludes with the limitations/challenges and future directions.

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“…Nanotechnology has transformed the biomedical diagnosis industry and detection and sensing of biomolecules using enzyme-based sensors have been used in the detection of various biomolecules, including Covid-19, recently. , Hydrogen peroxide (H 2 O 2 ), a crucial reactive oxygen species (ROS), has been linked with several disorders, including cancer, neurological disorders, and Alzheimer’s disease . Since H 2 O 2 acts as an oxidizing agent, its higher levels in cells trigger the onset of cellular damage through the oxidation of DNA, proteins, and lipids .…”

Section: Introductionmentioning

confidence: 99%

PBA-Derived Heteroatom-Doped Mesoporous Graphitic Spheroids as Peroxidase Nanozyme for In Vitro Tumor Cells Detection

Sahar,

Zeb,

Mao

et al. 2024

ACS Appl. Bio Mater.

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Inspired by the two kinds of naturally occurring peroxidases (POD) with vanadium or heme (iron)-based active catalytic centers, we have developed a dual metal-based nanozyme with dual V and Fe-based active catalytic centers. Co-doping of graphene with heteroatoms has a synergistic effect on the catalytic properties of the nanomaterial as the distances of migration of the substrates drastically reduce. However, a few studies have reported the codoping of heterometallic elements in the graphene structure due to the complexity of the synthesis procedures. Herein, we report the synthesis of in situ doped bimetallic VNFe@C mesoporous graphitic spheroids nanozyme via pyrolysis without the assistance of any template assisted method. The Prussian-blue analog-based precursor material was synthesized by a facile one-step low-temperature synthesis procedure. The bimetallic spheroids showed an excellent affinity toward H 2 O 2 , with a K m value of 0.26 mM when compared to 0.436 for the natural POD, which is much better than the natural POD, which was utilized to detect tumor cells in vitro through the intracellular H 2 O 2 produced by these cells under high oxidative stress. The VNFe@C mesoporous spheroids generate dual reactive oxygen species, including the • OH and • O 2 H − radicals, in the presence of H 2 O 2 , which are responsible for the POD-like activity of these nanozymes, while the bimetallic V/Fe doping plays a synergistic role in the enhancement of the activity of codoped graphitic spheroids.

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Aptamer‐based strategies against SARS‐CoV‐2 viruses

Cited by 6 publications

References 87 publications

Aptamers and Nanobodies as New Bioprobes for SARS-CoV-2 Diagnostic and Therapeutic System Applications

Aptamers and Nanobodies as New Bioprobes for SARS-CoV-2 Diagnostic and Therapeutic System Applications

DNA and Nanomaterials: A Functional Combination for DNA Sensing

PBA-Derived Heteroatom-Doped Mesoporous Graphitic Spheroids as Peroxidase Nanozyme for In Vitro Tumor Cells Detection

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