Multi-spectroscopic analysis of cholesterol gallstone using TOF-SIMS, FTIR and UV–Vis spectroscopy

Jaswal, Brij Bir S.; Kumar, Vinay; Swart, H.C.; Sharma, J. M.; Pradeep, Konda Gokuldoss; Singh, Vivek K.

doi:10.1007/s00340-015-6200-3

Cited by 14 publications

(5 citation statements)

References 20 publications

(17 reference statements)

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“…Various techniques have been employed for the characterization of gallstone diseases in the past. X-ray diffraction [13], Fourier-transform infrared spectroscopy (FTIR) [11,14,15], particleinduced X-ray emission [16], scanning electron microscopy (SEM) [17,18], magnetic resonance imaging (MRI) [19] and nuclear magnetic resonance (NMR) spectroscopy [11,17,[20][21][22][23], have been used extensively to study the composition of gallstones and provide significant information to the scientific community. But still, we are nowhere close to the prevention of gallstones.…”

Section: Plos Onementioning

confidence: 99%

NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent

et al. 2023

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Analysis of the chemical composition of gallstones is vital for the etiopathogenesis of gallstone diseases that can ultimately help in the prevention of its formation. In the present study, gallstones from seven different regions of India were analyzed to highlight the major difference in their composition. Also, gallstones of different pathological conditions i.e., benign (chronic cholecystitis, CC) and malignant gallbladder disease (gallbladder cancer GBC) were characterized. The type of polymorphs of cholesterol molecules was also studied to provide insight into the structure of gallstones. 1H solution state NMR spectroscopy 1D experiments were performed on a total of 94 gallstone (GS) samples collected from seven different geographical regions of India. Solid-State NMR spectroscopy 13C cross-polarization magic angle spinning (CPMAS) experiments were done on the 20 CC GS samples and 20 GBC GS samples of two regions. 1H NMR spectra from the solution state NMR of all the stones reveal that cholesterol was a major component of the maximum stones of the north India region while in south Indian regions, GS had very less cholesterol. 13C CPMAS experiments reveal that the quantity of cholesterol was significantly more in the GS of CC in the Lucknow region compared with GBC stones of Lucknow and Chandigarh. Our study also revealed that GS of the Lucknow region of both malignant and benign gallbladder diseases belong to the monohydrate crystalline form of cholesterol while GS of Chandigarh region of both malignant and benign gallbladder diseases exists in both monohydrate crystalline form with the amorphous type and anhydrous form. Gallstones have a complicated and poorly understood etiology. Therefore, it is important to understand the composition of gallstones, which can be found in various forms and clinical conditions. Variations in dietary practices, environmental conditions, and genetic factors may influence and contribute to the formation of GS. Prevention of gallstone formation may help in decreasing the cases of gallbladder cancer.

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Section: Plos Onementioning

confidence: 99%

NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent

et al. 2023

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“…In a series of studies, Jaswal et al (2015aJaswal et al ( , 2015bJaswal et al ( , 2016bJaswal et al ( , and 2019 performed spectroscopic studies to investigate elemental compositions of different kinds of gallstones. Before performing elemental studies, the authors used the FTIR technique to classify gallstone samples into different categories (cholesterol, mixed and pigment type).…”

Section: Studies On Stones Using Ftir Spectroscopymentioning

confidence: 99%

“…It is pertinent to mention that relatively few studies (Ghumman et al 2010(Ghumman et al , 2011(Ghumman et al , 2012(Ghumman et al , 2013 have been reported on the use and application of TOF-SIMS on human stones. Jaswal et al (2015b) analyzed the different parts of cholesterol type gallstones employing TOF-SIMS, employing 2D (two-dimensional) elemental images for the assessment of mineral distribution. The authors were able to analyze and obtain the data from 200 × 200 μm 2 of the area of stones.…”

Section: Studies On Stones Using Tof-sims Spectrometrymentioning

confidence: 99%

Analysis of stones formed in the human gall bladder and kidney using advanced spectroscopic techniques

Singh¹,

Jaswal²,

Sharma³

et al. 2020

Biophys Rev

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Stone diseases (gallstones and kidney stones) are extremely painful and often cause death. The prime aim of biomedical research in this area has been determination of factors resulting in stone formation inside the gallbladder and urinary tract. Many theories have been put forward to explain the mechanism of stone formation and their growth; however, their complete cycle of pathogenesis is still under debate. Several factors are responsible for stone formation; however, much emphasis is placed on the determination of elemental and molecular composition of the stones. In the present review article, we describe different kinds of spectroscopic techniques such as Fourier transform infrared spectroscopy (FTIR), X-ray fluorescence (XRF) spectroscopy, time-of-flight secondary ion mass spectrometry (TOF-SIMS), and laser-induced breakdown spectroscopy (LIBS) and highlight their use in the analysis of stone diseases. We have summarized work done on gallstones and kidney stones using these advanced techniques particularly over the last 10 years. We have also briefly elaborated the basics of stone formations inside the human body and their complications for a better understanding of the subject.

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“…FTIR spectroscopy was carried out to confirm the classification of GS which we have made with the help of visual aid [10]. The type of stone, the main chemical constituent of each type and its corresponding absorbing IR bands obtained along with literature values [5,[11][12][13][14][15][16][17] are given in (Table 6). Representative infrared spectra of cholesterol, pigmented and mixed gallstones are shown in Figs.…”

Section: Ftir Analysismentioning

confidence: 99%

Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study

Khan

Kazi

Afridi

et al. 2017

Clinica Chimica Acta

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Multi-spectroscopic analysis of cholesterol gallstone using TOF-SIMS, FTIR and UV–Vis spectroscopy

Cited by 14 publications

References 20 publications

NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent

NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent

Analysis of stones formed in the human gall bladder and kidney using advanced spectroscopic techniques

Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study

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