Multi-residue methodology for the determination of 16 coccidiostats in animal tissues and eggs by hydrophilic interaction liquid chromatography – Tandem mass spectrometry

Dasenaki, Marilena E.; Thomaidis, Nikolaos S.

doi:10.1016/j.foodchem.2018.09.138

Cited by 34 publications

(27 citation statements)

References 25 publications

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“…Particularly, the presence of coccidiostat residues in edible tissues and eggs has been reported in several countries since the late 1990s [39,40]. This has encouraged research and application of surveillance programs in many countries to monitor and prevent unacceptable contamination of food of animal origin [24]. Two hundred and two samples of meat of different species (5 bovine, 29 swine, 166 poultry, 1 ovine and 1 rabbit), and 151 of hen eggs were analysed for coccidiostat residues during 2012–2017 in the present study, in compliance with the NRCP.…”

Section: Resultsmentioning

confidence: 99%

“…The surveillance of coccidiostat residues in food has been focused mainly on ionophores and NIC in eggs and poultry meat [2] for a long time, probably related to limitations attributable to analytical methods. The availability of multi-residue analytical methods, suitable for the simultaneous determination of a wider range of coccidiostat residues in a wider range of species tissue, represents an undisputable advantage in the frame of official control [2,22,23,24].…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and Residue Concentration of Coccidiostats in Feed and Food of Animal Origin; Human Exposure Assessment

Roila

Branciari

Pecorelli

et al. 2019

Foods

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Occurring central Italy, 262 unmedicated feed samples and 353 samples of animal tissues and eggs are tested for coccidiostats between 2012 and 2017. A validated multi-residue HPLC-MS/MS method is applied for the simultaneous determination of the 11 coccidiostats licensed in the EU. The dietary exposure to coccidiostats through poultry meat and eggs is calculated for high consumers, and the contribution to acceptable daily intake of coccidiostats is evaluated. The occurrence of positive feed samples ranges from 17.2% in 2012 to 28.3% in 2017, with an average percentage of positive samples of 25%, while 3.8% of feed samples are non-compliant with a concentration ranging from 0.015 mg/kg for diclazuril to 56 mg/kg for narasin. Positive samples of animal tissues, on average, are 34.7%, fully compliant, while 16% of eggs are positive and violative residues are found in 2%. These noncompliant samples show a concentration varying from 2.4 µg/kg to 1002 µg/kg. The contribution of poultry meat and egg consumption to the acceptable daily intake of each coccidiostat is below 1%, highlighting a low direct risk to public health.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Occurrence and Residue Concentration of Coccidiostats in Feed and Food of Animal Origin; Human Exposure Assessment

Roila

Branciari

Pecorelli

et al. 2019

Foods

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show abstract

“…The analysis of the critical points in the internal processes has highlighted, as the main problem to be addressed, the outsourcing of some services such as the multiresidual analysis of products intended for food [13][14].…”

Section: Discussionmentioning

confidence: 99%

Innovative process methodologies for the agro-food sector: reorganization of technical processes to acquire new skills

Maurizi¹,

Evangelista²,

Sorrentino³

et al. 2019

asb

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“…However, due to the low UV and FLD absorbance of polyether antibiotics, it is often necessary for them to be derivatized for detection, which is not only time-consuming but the sample pretreatment becomes more complex. In addition, various analytical methods have been used to measure anticoccidial drugs in eggs, chicken tissue, milk, duck muscle, pork, beef, and other animal foods, including competitive enzyme-linked immunosorbent assays (cELISA) [13], micellar electrokinetic chromatography (MEKC) [14], flow cytometry-based immunoassays (FCIA) [15], hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) [16], and liquid chromatography-tandem mass spectrometry (LC-MS/MS) . Compared with other detection methods, the LC-MS/MS method has the advantages of qualitative and quantitative accuracy, it is fast and does not require analyte derivatization, and its sensitivity could allow for the detection of low-concentration substances.…”

Section: Introductionmentioning

confidence: 99%

Determination of Eight Coccidiostats in Eggs by Liquid–Liquid Extraction–Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

et al. 2020

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A method for the simultaneous determination of robenidine, halofuginone, lasalocid, monensin, nigericin, salinomycin, narasin, and maduramicin residues in eggs by liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed. The sample preparation method used a combination of liquid–liquid extraction (LLE) and solid-phase extraction (SPE) technology to extract and purify these target compounds from eggs. The target compounds were separated by gradient elution using high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC). Tandem mass spectrometry was used to quantitatively and qualitatively analyze the target compounds via electrospray ionization (ESI+) and multiple reaction monitoring mode. The HPLC–MS/MS and UPLC–MS/MS methods were validated according to the requirements defined by the European Union and the Food and Drug Administration. The limits of detection and limits of quantification of the eight coccidiostats in eggs were 0.23–0.52 µg/kg and 0.82–1.73 µg/kg for HPLC–MS/MS, and 0.16-0.42 µg/kg and 0.81-1.25 µg/kg for UPLC–MS/MS, respectively. The eggs were spiked with four concentrations of the eight coccidiostats, and the HPLC–MS/MS and UPLC–MS/MS average recoveries were all higher than 71.69% and 72.26%, respectively. Compared with the HPLC–MS/MS method, utilizing UPLC–MS/MS had the advantages of low reagent consumption, a short detection time, and high recovery and precision. Finally, the HPLC–MS/MS and UPLC–MS/MS methods were successfully applied to detect eight coccidiostats in 40 eggs.

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Multi-residue methodology for the determination of 16 coccidiostats in animal tissues and eggs by hydrophilic interaction liquid chromatography – Tandem mass spectrometry

Cited by 34 publications

References 25 publications

Occurrence and Residue Concentration of Coccidiostats in Feed and Food of Animal Origin; Human Exposure Assessment

Occurrence and Residue Concentration of Coccidiostats in Feed and Food of Animal Origin; Human Exposure Assessment

Innovative process methodologies for the agro-food sector: reorganization of technical processes to acquire new skills

Determination of Eight Coccidiostats in Eggs by Liquid–Liquid Extraction–Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

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