Mucus glycoprotein of human saliva: differences in the associated and covalently bound lipids with caries

Slomiany, Bronislaw L.; Murty, V.L.N.; Slomiany, Amalia; Zielénski, J.; Mandel, Irwin D.

doi:10.1016/0304-4165(86)90050-4

Cited by 43 publications

(20 citation statements)

References 28 publications

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“…In this study, we have been able to establish that the membrane FBP contains covalently bound fatty acids (i.e., (18). That the micellar aggregation of the membrane FBP with Triton X-100 (which was demonstrated by gel filtration) was still observed following mild treatment with hydroxylamine (pH 9.8 at 220C) is consistent with the greater fraction of amide-linked fatty acid since these bonds are not cleaved by hydoxylamine under these conditions (29).…”

Section: Discussionsupporting

confidence: 58%

“…The procedure was repeated two more times, and the delipidated protein material contained in the aqueous phase was dialyzed, Iyophilized, and subjected to analysis of covalently bound fatty acids. For the analysis of ester-bound fatty acids, the delipidated sample was incubated for 30 min at 37°C with 0.3 M methanolic KOH (18). Acid methanolysis in 1.0 M methanolic HCI at 80°C for 24 hr was used to release the amide-and ester-bound fatty acids (18).…”

mentioning

confidence: 99%

“…For the analysis of ester-bound fatty acids, the delipidated sample was incubated for 30 min at 37°C with 0.3 M methanolic KOH (18). Acid methanolysis in 1.0 M methanolic HCI at 80°C for 24 hr was used to release the amide-and ester-bound fatty acids (18). The released fatty acid methyl esters were recovered from the reaction mixtures by three consecutive extractions with hexane and analyzed by gas/liquid chromatography using nonadecanoic acid as the internal standard.…”

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confidence: 99%

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Purified membrane and soluble folate binding proteins from cultured KB cells have similar amino acid compositions and molecular weights but differ in fatty acid acylation.

Luhrs¹,

Pitiranggon²,

Costa³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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A membrane-associated folate binding protein (FBP) and a soluble FBP, which is released into the culture medium, have been purified from human KB cells using affinity chromatography. By NaDodSO4/PAGE, both proteins have an apparent Mr of -42,000. However, in the presence of Triton X-100, the soluble FBP eluted from a Sephadex G-150 column with an apparent Mr of =40,000 (similar to NaDodSO4/PAGE) but the membrane-associated FBP eluted with an apparent Mr of -160,000, indicating that this species contains a hydrophobic domain that interacts with the detergent micelles. The amino acid compositions of both forms of FBP were similar, especially with respect to the apolar amino acids. In addition, the 18 amino acids at the amino termini of both proteins were identical. The membrane FBP, following delipidation with

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Section: Discussionsupporting

confidence: 58%

mentioning

confidence: 99%

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confidence: 99%

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Purified membrane and soluble folate binding proteins from cultured KB cells have similar amino acid compositions and molecular weights but differ in fatty acid acylation.

Luhrs¹,

Pitiranggon²,

Costa³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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“…It was put forward that fatty acids had been demonstrated in salivary mucous glycoprotein and covalently bound. These fatty acids were ester linked to the nonglycosylated regions of mucous glycoprotein were comprised primary of hexadecanoic, octadecanoic and docosanoic acids 23,24) . But the interaction and variations between lipid and protein components in the saliva, and caries experience have been scarce.…”

Section: Y Tomita N Miyake and S Yamanakamentioning

confidence: 99%

Lipids in Human Parotid Saliva with Regard to Caries Experience

Tomita¹,

Miyake²,

Yamanaka

2008

J. Oleo Sci.

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It has been reported that saliva may play an important role in the prevention and development of enamel caries and that both lipids and protein contents in saliva may be relevant to this role. This study examined the lipid and protein levels in saliva from individuals differing in caries experience. Female subjects (20 to 21 years old) were used divided equally into two groups, caries-susceptible group (CSG) and caries-resistant group (CRG). Stimulated parotid saliva and stimulated whole saliva were collected from the subjects. After centrifugation, each saliva sample was analyzed for the concentrations of lipids and proteins and for the compositions of lipids and fatty acids. The lipid and protein contents in parotid saliva increased in proportion to increase of the flow rate. The lipid content was slightly correlated with the protein one (r = 0.33). Total lipid and protein concentrations were higher in the samples from CSG than those from CRG. The lipid composition was similar in the samples from the two groups; more than half in total lipids was neutral lipids, followed by glycolipids and phospholipids. Neutral lipids and free fatty acid and triacylglyceride concentrations were significantly higher in the samples from CSG than those from CRG (p<0.01 for each). Also stearic, linoleic and docosahexaenoic acids were significantly higher in the former group than the latter one (p<0.05 and p<0.01). In summary, the lipid concentrations in parotid saliva from caries susceptible subjects were higher than those from caries resistant ones, and the difference in fatty acid composition was detected between them. The variations in the lipid levels and fatty acid composition may be associated with those in caries development.

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“…32 In this respect, it is interesting to note that associated lipids appear to play a role in determining the susceptibility of salivary mucins to proteolysis. 18 Alternatively, it is conceivable that MG2 is displaced by other pellicular components such as the cy statins. Previously, we have shown that the adsorption of MG2 to hydroxy apatite, in vitro, is markedly inhibited by the salivary cystatins.…”

Section: A Tissue Coatingmentioning

confidence: 99%