Mucosal pathology of the eustachian tube after exposure to sulfur dioxide

We have used a tissue culture technique and a photoelectric method to examine the direct effect of lipopolysaccharide (LPS) on the ciliary activity present in the eustachian tube. Since LPS possesses the major part of the biological activity of endotoxin, our results show clearly that LPS deteriorates the ciliary activity in a dose-response fashion: LPS does not deteriorate the ciliary activity up to 168 h if its concentration is 1 ng/ml or less; 10 ng/ml LPS can cause deterioration of the ciliary activity with extended exposure (more than 96 h); LPS can cause dysfunction of the cilia rather quickly if the concentration is 100 ng/ml or more. Our results show that the ciliary activity in the eustachian tube under clinical conditions can be affected by endotoxin.

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Effects of bacterial endotoxin on the ciliary activity in the in vitro eustachian tube

Ōhashi

Nakai

Ikeoka

et al. 1987

Arch Otorhinolaryngol

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Ciliary activity in the in vitro tubotympanum

Ōhashi

Nakai

Koshimo

et al. 1986

Arch Otorhinolaryngol

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We have introduced a tissue culture technique in which the ciliary activity of the cultured tubotympanum can be studied. We have found that ciliated cells of the eustachian tube and middle ear lining continued a beating activity during 168 h of observation. The mean loss of activity of the ciliated cells from the eustachian tube or mucosal sites proximal to the tube was 20% or less, while that of mucosal sites distal to the tube was about 40% even after 168 h of cultivation in our culture medium of RPMI 1640. Our technique of tissue culture is available for studying the long-term effects of various therapeutic agents or adverse factors on the ciliary activity in the tubotympanum.

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Pharyngeal ciliary activity in guinea pigs with otitis media

Ōhashi

Nakai

Koshimo

et al. 1988

Arch Otorhinolaryngol

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The function of cilia present in the tubotympanum plays an important role in the pathogenesis and chronicity of otitis media with effusion (OME). We used a photoelectric method to examine the ciliary activity in the eustachian tube, in the central pharynx, as well as the nasal cavity of normal animals and animals with otitis media. In normal animals, the ciliary activity in the nasal cavity was not different from that in the eustachian tube. In some animals with otitis media, however, there was a significant difference between the ciliary activity in the nasal cavity and that in the eustachian tube. On the other hand, no significant differences were recognized in any given group of animals examined between the level of the ciliary activity in the central pharynx and that in the eustachian tube. Our findings show that the function of the cilia present in the pharynx is a valid index of that in the eustachian tube. Our study further indicates that it may be possible to clinically assess the ciliary activity in the pharyngeal mucosa biopsied from patients with OME to determine objectively the effects of medical treatments of the disease.

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Mucosal pathology of the eustachian tube after exposure to sulfur dioxide

Cited by 14 publications

References 16 publications

Effects of bacterial endotoxin on the ciliary activity in the in vitro eustachian tube

Effects of bacterial endotoxin on the ciliary activity in the in vitro eustachian tube

Ciliary activity in the in vitro tubotympanum

Pharyngeal ciliary activity in guinea pigs with otitis media

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